Feeding conditions control the expression of genes involved in sterol metabolism in peripheral blood mononuclear cells of normoweight and diet-induced (cafeteria) obese rats

2010 ◽  
Vol 21 (11) ◽  
pp. 1127-1133 ◽  
Author(s):  
Antoni Caimari ◽  
Paula Oliver ◽  
Wendy Rodenburg ◽  
Jaap Keijer ◽  
Andreu Palou
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Sterol Metabolism ◽  
Peripheral Blood Mononuclear ◽  
Expression Of Genes ◽  
Blood Mononuclear Cells ◽  
Obese Rats

scholarly journals Trypanosoma cruziSSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Yadira Morán-Utrera ◽  
Aracely López-Monteon ◽  
José Luis Rosales-Encina ◽  
Enrique Méndez-Bolaina ◽  
Angel Ramos-Ligonio
Keyword(s):  
Chagas Disease ◽  
Acute Phase ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
No Production ◽  
Peripheral Blood Mononuclear ◽  
Expression Of Genes ◽  
Blood Mononuclear Cells ◽  
Host Parasite

The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in whichTrypanosoma cruzireplicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the parasite to spread in the host. Parasite molecules that regulate the host immune response during Chagas’ disease have not been fully identified, particularly proteins of the amastigote stage. In this work, we evaluated the role of the GPI anchored SSP4 protein ofT. cruzias an immunomodulatory molecule in peripheral blood mononuclear cells (PBMCs). rMBP::SSP4 protein was able to stimulate nitric oxide (NO) production. Likewise, rMBP::SSP4 induced the expression of genes and production of molecules involved in the inflammatory process, such as, cytokines, chemokines, and adhesion molecules (CAMs) as determined by RT-PCR and ELISA. These results suggest that the amastigote SSP4 molecule could play a key role in the immunoregulatory and/or immunosuppressive process observed in the acute phase of infection withT. cruzi.

scholarly journals Fasciola gigantica–Derived Excretory-Secretory Products Alter the Expression of mRNAs, miRNAs, lncRNAs, and circRNAs Involved in the Immune Response and Metabolism in Goat Peripheral Blood Mononuclear Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Sha-Sha Wang ◽  
Dan Chen ◽  
Jun-Jun He ◽  
Wen-Bin Zheng ◽  
Ai-Ling Tian ◽  
...  
Keyword(s):  
Immune Response ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Fasciola Gigantica ◽  
Rna Seq ◽  
Peripheral Blood Mononuclear ◽  
Expression Of Genes ◽  
Blood Mononuclear Cells ◽  
Secretory Products

Fasciola gigantica produces excretory-secretory products (ESPs) with immune-modulating effects to promote its own survival. In this study, we performed RNA-seq to gain a comprehensive global understanding of changes in the expression of mRNAs, miRNAs, lncRNAs, and circRNAs in goat peripheral blood mononuclear cells (PBMCs) treated with F. gigantica ESPs. A total of 1,544 differently expressed mRNAs (790 upregulated and 754 downregulated genes), 30 differently expressed miRNAs (24 upregulated and 6 downregulated genes), 136 differently expressed circRNAs (83 upregulated and 53 downregulated genes), and 1,194 differently expressed lncRNAs (215 upregulated and 979 downregulated genes) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that F. gigantica ESPs altered the expression of genes associated with the host immune response, receptor signaling, disease and metabolism. Results from RNA-seq were validated by qRT-PCR. These findings provide an important resource for future investigation of the role of mRNAs and non-coding RNAs in mediating the immune-modulating effects of F. gigantica ESPs.

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