Proinflammatory cytokine mRNA expression in mammary tissue of sows following intramammary inoculation with Escherichia coli

2007 ◽  
Vol 116 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Yaohong Zhu ◽  
Mikael Berg ◽  
Caroline Fossum ◽  
Ulf Magnusson
Keyword(s):  
Escherichia Coli ◽  
Mrna Expression ◽  
Proinflammatory Cytokine ◽  
Mammary Tissue ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

Maternal glucocorticoids increase endotoxin-induced lung inflammation in preterm lambs

2003 ◽  
Vol 284 (4) ◽  
pp. L633-L642 ◽  
Author(s):  
Suhas G. Kallapur ◽  
Boris W. Kramer ◽  
Timothy J. M. Moss ◽  
John P. Newnham ◽  
Alan H. Jobe ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Proinflammatory Cytokine ◽  
Lung Inflammation ◽  
Inflammatory Cells ◽  
Bronchial Epithelium ◽  
Saline Control ◽  
Cytokine Mrna ◽  
Lung Volumes ◽  
Cytokine Mrna Expression ◽  
Beta 2

Antenatal betamethasone (Beta) is widely used in women with asymptomatic chorioamnionitis at risk for preterm delivery, but its effects on fetal inflammation are unstudied. Groups of ewes at 109 ± 1 days of gestation received the following treatments: intra-amniotic (IA) saline (control), 0.5 mg/kg intramuscular Beta, 10 mg IA endotoxin (Endo), and Beta + 2 h later Endo (Beta + Endo). Beta suppressed Endo-induced lung inflammation at 1 day. However, compared with Endo 5 days after treatment, Beta + Endo lambs had increased alveolar neutrophils, proinflammatory cytokine mRNA expression, and serum amyloid A3 (SAA3) mRNA expression. IL-1β mRNA expression was localized to the inflammatory cells, whereas SAA3 mRNA expression was induced in the bronchial epithelium and the inflammatory cells. Compared with Endo, Beta + Endo lambs had increased lung inflammation but equivalent lung volumes 15 days after treatment. The late increase in inflammation in the Beta + Endo animals suggests that glucocorticoids impair the ability of the preterm lung to downregulate Endo-induced inflammation after fetal clearance of the glucocorticoids. These results have implications for lung inflammation and bronchopulmonary dysplasia in preterm infants exposed to chorioamnionitis and maternal glucocorticoids.

Cytokine mRNA expression in porcine cell lines stimulated by enterotoxigenic Escherichia coli

2008 ◽  
Vol 132 (1-2) ◽  
pp. 105-110 ◽  
Author(s):  
Barbora Pavlova ◽  
Jiri Volf ◽  
Pavel Alexa ◽  
Ivan Rychlik ◽  
Jan Matiasovic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mrna Expression ◽  
Cell Lines ◽  
Enterotoxigenic Escherichia Coli ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Porcine Cell
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