High concentration of human lactoferrin in milk of rhLf-transgenic cows relieves signs of bovine experimental Staphylococcus chromogenes intramammary infection

2010 ◽  
Vol 136 (3-4) ◽  
pp. 265-271 ◽  
Author(s):  
Heli Simojoki ◽  
Paula Hyvönen ◽  
Toomas Orro ◽  
Satu Pyörälä
Keyword(s):  
Human Lactoferrin ◽  
High Concentration ◽  
Intramammary Infection ◽  
Transgenic Cows

scholarly journals Transgenic Cows That Produce Recombinant Human Lactoferrin in Milk Are Not Protected from Experimental Escherichia coli Intramammary Infection

2006 ◽  
Vol 74 (11) ◽  
pp. 6206-6212 ◽  
Author(s):  
P. Hyvönen ◽  
L. Suojala ◽  
T. Orro ◽  
J. Haaranen ◽  
O. Simola ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Serum Cortisol ◽  
Clinical Mastitis ◽  
Human Lactoferrin ◽  
Protective Effects ◽  
Recombinant Human Lactoferrin ◽  
Intramammary Infection ◽  
Transgenic Cows

ABSTRACT This is the first study describing an experimental mastitis model using transgenic cows expressing recombinant human lactoferrin (rhLf) in their milk. The aim of the study was to investigate the concentrations in milk and protective effects of bovine and recombinant human lactoferrin in experimental Escherichia coli mastitis. Experimental intramammary infection was induced in one udder quarter of seven first-lactating rhLf-transgenic cows and six normal cows, using an E. coli strain isolated from cows with clinical mastitis and known to be susceptible to Lf in vitro. Clinical signs were recorded during the experimental period, concentrations of human and bovine Lf and indicators of inflammation and bacterial counts were determined for milk, and concentrations of acute-phase proteins and tumor necrosis factor alpha were determined for sera and milk. Serum cortisol and blood hematological and biochemical parameters were also determined. Expression levels of rhLf in the milk of transgenic cows remained constant throughout the experiment (mean, 2.9 mg/ml). The high Lf concentrations in the milk of transgenic cows did not protect them from intramammary infection. All cows became infected and developed clinical mastitis. The rhLf-transgenic cows showed milder systemic signs and lower serum cortisol and haptoglobin concentrations than did controls. This may be explained by lipopolysaccharide-neutralizing and immunomodulatory effects of the high Lf concentrations in their milk. However, Lf does not seem to be a very efficient protein for genetic engineering to enhance the mastitis resistance of dairy cows.

scholarly journals Characterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171477 ◽  
Author(s):  
Annabelle Le Parc ◽  
Sercan Karav ◽  
Camille Rouquié ◽  
Elizabeth A. Maga ◽  
Apichaya Bunyatratchata ◽  
...  
Keyword(s):  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin ◽  
Transgenic Cows

The effect of recombinant human lactoferrin from the milk of transgenic cows on Salmonella enterica serovar typhimurium infection in mice

2016 ◽  
Vol 7 (1) ◽  
pp. 308-314 ◽  
Author(s):  
Jinghuan Wu ◽  
Yujie Hu ◽  
Chunming Du ◽  
Jianhua Piao ◽  
Lichen Yang ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Antibacterial Effect ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin ◽  
Serovar Typhimurium ◽  
Transgenic Cows

Recombinant human lactoferrin from the milk of transgenic cows has an antibacterial effect of alleviating infection againstSalmonella entericaserovartyphimuriumin BALB/c mice.

Sub-chronic (13-week) oral toxicity study in rats with recombinant human lactoferrin produced in the milk of transgenic cows

2006 ◽  
Vol 44 (7) ◽  
pp. 964-973 ◽  
Author(s):  
M.J. Appel ◽  
H.A. van Veen ◽  
H. Vietsch ◽  
M. Salaheddine ◽  
J.H. Nuijens ◽  
...  
Keyword(s):  
Toxicity Study ◽  
Human Lactoferrin ◽  
Oral Toxicity ◽  
Recombinant Human Lactoferrin ◽  
Transgenic Cows

The protein structure of recombinant human lactoferrin produced in the milk of transgenic cows closely matches the structure of human milk-derived lactoferrin

2005 ◽  
Vol 14 (4) ◽  
pp. 397-405 ◽  
Author(s):  
Ellen A. J. Thomassen ◽  
Harrie A. van. Veen ◽  
Patrick H. C. van. Berkel ◽  
Jan H. Nuijens ◽  
Jan Pieter Abrahams
Keyword(s):  
Protein Structure ◽  
Human Milk ◽  
Human Lactoferrin ◽  
Recombinant Human Lactoferrin ◽  
Transgenic Cows

Large scale production of recombinant human lactoferrin in the milk of transgenic cows

2002 ◽  
Vol 20 (5) ◽  
pp. 484-487 ◽  
Author(s):  
Patrick H.C. van Berkel ◽  
Mick M. Welling ◽  
Marlieke Geerts ◽  
Harry A. van Veen ◽  
Bep Ravensbergen ◽  
...  
Keyword(s):  
Large Scale ◽  
Human Lactoferrin ◽  
Scale Production ◽  
Recombinant Human Lactoferrin ◽  
Large Scale Production ◽  
Transgenic Cows

Cytomembrane Preservation in Tissue Dehydrated Only With Glutaraldehyde and Epon 812 Resin

1973 ◽  
Vol 31 ◽  
pp. 320-321
Author(s):  
Daniel C. Pease
Keyword(s):  
Diffraction Pattern ◽  
X Ray Diffraction ◽  
High Concentration ◽  
Unpublished Study ◽  
X Ray ◽  
Sectioned Material

A previous study demonstrated that tissue could be successfully infiltrated with 50% glutaraldehyde, and then subsequently polymerized with urea to create an embedment which retained cytomembrane lipids in sectioned material. As a result, the 180-190 Å periodicity characteristic of fresh, mammalian myelin was preserved in sections, as was a brilliant birefringence, and the capacity to bind OsO4 vapor in the hydrophobic bilayers. An associated (unpublished) study, carried out in co-operation with Drs. C.K. Akers and D.F. Parsons, demonstrated that the high concentration of glutaraldehyde (and urea) did not significantly alter the X-ray diffraction pattern of aldehyde-fixed, myelin. Thus, by itself, 50% glutaraldehyde has little effect upon cytomembrane systems and can be used with confidence for the first stages of dehydration.

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