Development of an indirect ELISA test using a purified tachyzoite surface antigen SAG1 for sero-diagnosis of canine Toxoplasma gondii infection

2009 ◽  
Vol 164 (2-4) ◽  
pp. 315-319 ◽  
Author(s):  
M. Hosseininejad ◽  
H.R. Azizi ◽  
F. Hosseini ◽  
G. Schares
Keyword(s):  
Toxoplasma Gondii ◽  
Surface Antigen ◽  
Indirect Elisa ◽  
Elisa Test ◽  
Toxoplasma Gondii Infection

scholarly journals An investigation of sheep toxoplasmosis in Basrah Province / Iraq

2010 ◽  
Vol 9 (3) ◽  
pp. 128
Author(s):  
K. M. Al-Saad ◽  
Saad Hashim Al-Husseiny
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Elisa Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Results ◽  
Significant Difference ◽  
The Difference

The objective of this study was to investigate Toxoplasma gondii antibodies among sheep in different regions of Basrah province (including Al-Mdayna, Shatt Al-Arab, Al-Basrah, Al-Zubayr, and Abu Al-Khasib). The study was started in Oct. 2008 and was finished in May 2009, using latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) IgG, 309 adult sheep were randomly selected from 15 herds among different ages and both sexes and used in this study, including 62 pregnant ewes, 185 non-pregnant ewes, 14 aborted ewes, and 48 rams. Results showed, that 60.84% were seropositive by LAT, whereas 51.11% were seropositive by ELISA IgG test, among animals used in this study, results detected that 79.03% pregnant ewes (highest value), 56.75% nonpregnant ewes,71.40% aborted ewes and 50% Rams (lowest value) were seropositive by LAT, whereas 56.52% pregnant ewes, 51.11% non-pregnant ewes, 83.33% aborted ewes (highest value), and 31.25% Rams (lowest value) were seropositive by indirect ELISA IgG. Moreover, among regions of Basrah province, the details of percentage of T.gondii antibodies were 54.54% in AL-Basrah , 71.43% in Abu Al-Khasib (highest value), 57.35% in Al- Mdayna, 47.83% in Shatt Al-Arab (lowest value), and 67.16% in Al-Zubayr by LAT, whereas 63.64% in AL-Basrah (highest value), 22.73% in Abu Al-Khasib (lowest value), 57.89% in Al-Mdayna, 50% in Shatt Al-Arab and 61.90% in Al-Zubayr by indirect ELISA test. Although the difference observed in the percentage of Toxoplasma gondii antibodies among different regions of Basrah, there was no significant difference P>0.05 detected LAT, whereas in the indirect ELISA IgG test there was significant difference P<0.05. Ewes showed high percentages 62.83%, 55.40% of toxoplasmosis than rams 50 %, 31.25% by LAT and ELISA test respectively. The highest titer was 1/4 28.57% were detected in pregnant ewes and lowest titers were 1/2, 1/8, and 1/256 0.0% were detected in aborted ewes and in ramsrespectively.

Development of an indirect ELISA test using an affinity purified surface antigen (P38) for sero-diagnosis of canine Neospora caninum infection

2010 ◽  
Vol 171 (3-4) ◽  
pp. 337-342 ◽  
Author(s):  
M. Hosseininejad ◽  
F. Hosseini ◽  
M. Mosharraf ◽  
S. Shahbaz ◽  
M. Mahzounieh ◽  
...  
Keyword(s):  
Surface Antigen ◽  
Indirect Elisa ◽  
Neospora Caninum ◽  
Elisa Test ◽  
Caninum Infection

scholarly journals Seroprevalence of Toxoplasma gondii infection in sheep in Inner Mongolia Province, China

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 11 ◽  
Author(s):  
Xinlei Yan ◽  
Wenying Han ◽  
Yang Wang ◽  
Hongbo Zhang ◽  
Zhihui Gao
Keyword(s):  
Toxoplasma Gondii ◽  
Inner Mongolia ◽  
Animal Husbandry ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Zoonotic Parasite ◽  
Health And Disease ◽  
Toxoplasma Gondii Infection ◽  
Almost All

Toxoplasma gondii is an important zoonotic parasite that can infect almost all warm-blooded animals, including humans, and infection may result in many adverse effects on animal husbandry production. Animal husbandry in Inner Mongolia is well developed, but data on T. gondii infection in sheep are lacking. In this study, we determined the seroprevalence and risk factors associated with the seroprevalence of T. gondii using an indirect enzyme-linked immunosorbent assay (ELISA) test. A total of 1853 serum samples were collected from 29 counties of Xilin Gol League (n = 624), Hohhot City (n = 225), Ordos City (n = 158), Wulanchabu City (n = 144), Bayan Nur City (n = 114) and Hulunbeir City (n = 588). The overall seroprevalence of T. gondii was 15.43%. Risk factor analysis showed that seroprevalence was higher in sheep ≥12 months of age (21.85%) than that in sheep <12 months of age (10.20%) (p < 0.01). Seroprevalence was higher in male sheep (18.76%) than females (12.80%) (p < 0.01). Barn-feeding sheep (23.13%) had higher prevalence than grazing sheep (10.94%) (p < 0.01). The seroprevalence was significantly different in different districts (p < 0.01). This study shows that sheep are exposed to T. gondii in Inner Mongolia, and provides a data reference for public health and disease control.

DNA vaccination with the immunodominant tachyzoite surface antigen (SAG-1) protects against adult acquired Toxoplasma gondii infection but does not prevent maternofoetal transmission

Vaccine ◽  
2003 ◽  
Vol 21 (21-22) ◽  
pp. 2813-2820 ◽  
Author(s):  
Kevin N. Couper ◽  
Henrik V. Nielsen ◽  
Eskild Petersen ◽  
Fiona Roberts ◽  
Craig W. Roberts ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Surface Antigen ◽  
Dna Vaccination ◽  
Toxoplasma Gondii Infection

scholarly journals Evaluation of the Effects of Toxoplasmosis in Small Ruminants on the Epidemiology of Human Toxoplasmosis by Indirect ELISA in Kaleybar Region, North West of Iran

2020 ◽  
Vol 1 (3) ◽  
pp. 65-69
Author(s):  
Yaghoub Firouzivand ◽  
Behrad Khan Ahmadi ◽  
Mustafa Afsari
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Elisa ◽  
Small Ruminants ◽  
Human Infection ◽  
Chi Square ◽  
Sheep And Goats ◽  
North West ◽  
Significant Difference ◽  
East Azerbaijan ◽  
Toxoplasma Gondii Infection

Introduction: Toxoplasma gondii is an intracellular protozoan that causes toxoplasmosis in humans and domestic animals. It causes abortion, especially in small ruminants. Sheep and goats have a significant role in the maintenance and survival of the parasite as well as its transmission to humans. Therefore, the aim of this study was to evaluate the seroprevalence of toxoplasmosis in small ruminants in Kaleybar region, East Azerbaijan province, using Indirect ELISA. Materials and Methods: In this study, blood samples of 270 sheep and goats were collected from October 2019 to December 2019 and anti-Toxoplasma IgG antibodies in sera were evaluated using indirect ELISA. Data analysis was performed using chi-square test in SPSS version 24.0. A P value of less than 0.05 was considered statistically significant. Results: Totally, 26 of 270 (9.62%) animals had anti-Toxoplasma gondii antibodies in their sera. Analysis showed that sheep were more susceptible to toxoplasmosis than goats and 21 (10.5%) sheep and 5 (7.14%) goats were seropositive for T. gondii; however, there was no statistically significant difference (P=0.413). The highest frequency (15.87%) of infection was observed in sheep and goats aged 3-4 years, respectively. Moreover, the rate of infection was higher in female (22/205) animals than in males (4/65); however, the relationship between gender and age and the frequency of positive samples was not significant (P=0.276 and P=0.121). Conclusions: The results of this study indicated that the seroprevalence of Toxoplasma gondii infection in small ruminants of the Kaleybar region (North West of Iran) is relatively low compared to other parts of the country. Further studies should be conducted on the rate of infection in definitive hosts of T. gondii in the region as a potential source of human infection.

scholarly journals Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1146 ◽  
Author(s):  
Lucyna Holec-Gąsior ◽  
Bartłomiej Ferra ◽  
Weronika Grąźlewska
Keyword(s):  
Toxoplasma Gondii ◽  
Small Ruminants ◽  
Chimeric Protein ◽  
High Specificity ◽  
Elisa Test ◽  
Dense Granule ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chimeric Proteins ◽  
Apical Membrane Antigen 1 ◽  
Toxoplasma Gondii Infection

The detection of Toxoplasma gondii infection in small ruminants has important significance for public health and veterinary medicine. This study, for the first time, describes the reactivity of four tetravalent chimeric proteins (AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, AMA1-SAG2-GRA1-ROP1, and SAG2-GRA1-ROP1-GRA2) containing immunodominant regions from the AMA1 (apical membrane antigen 1), SAG2 (surface antigen 2), GRA1 (dense granule antigen 1), GRA2 (dense granule antigen 2), and ROP1 (rhoptry antigen 1) with specific IgG antibodies from the sera of small ruminants with the use of an indirect enzyme-linked immunosorbent assay (ELISA). The reactivity of individual chimeric antigens was analyzed in relation to the results obtained in IgG ELISA based on a Toxoplasma lysate antigen (TLA). All chimeric proteins were characterized by high specificity (between 96.39% to 100%), whereas the sensitivity of the IgG ELISAs was variable (between 78.49% and 96.77%). The highest sensitivity was observed in the IgG ELISA test based on the AMA1-SAG2-GRA1-ROP1. These data demonstrate that this chimeric protein can be a promising serodiagnostic tool for T. gondii infection in small ruminants.

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