Defective DNAs of beet curly top virus from long-term survivor sugar beet plants

AbstractDiversity of the gut microbiome is associated with higher response rates for cancer patients receiving immunotherapy but has not been investigated in patients receiving radiation therapy. Additionally, current studies investigating the gut microbiome and outcomes in cancer patients may not have adjusted for established risk factors. Here, we sought to determine if diversity and composition of the gut microbiome was independently associated with survival in cervical cancer patients receiving chemoradiation. Our study demonstrates that the diversity of gut microbiota is associated with a favorable response to chemoradiation. Additionally, compositional variation among patients correlated with short term and long-term survival. Short term survivor fecal samples were significantly enriched in Porphyromonas, Porphyromonadaceae, and Dialister, whereas long term survivor samples were significantly enriched in Escherichia Shigella, Enterobacteriaceae, and Enterobacteriales. Moreover, analysis of immune cells from cervical tumor brush samples by flow cytometry revealed that patients with a high microbiome diversity had increased tumor infiltration of CD4+ lymphocytes as well as activated subsets of CD4 cells expressing ki67+ and CD69+ over the course of radiation therapy. Modulation of the gut microbiota before chemoradiation might provide an alternative way to enhance treatment efficacy and improve treatment outcomes in cervical cancer patients.

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Long-term survivor of human immunodeficiency virus-associated plasmablastic lymphoma

Indian Journal of Medical and Paediatric Oncology ◽

10.4103/0971-5851.116187 ◽

2013 ◽

Vol 34 (2) ◽

pp. 96 ◽

Cited By ~ 6

Author(s):

Atul Sharma ◽

T. V. S. V. G. K. Tilak ◽

Rakesh Lodha ◽

MC Sharma ◽

Deepak Dabkara

Keyword(s):

Human Immunodeficiency Virus ◽

Plasmablastic Lymphoma ◽

Immunodeficiency Virus ◽

Term Survivor

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Sanfilippo syndrome type C: A clinicopathological autopsy study of a long-term survivor

Pediatric Neurology ◽

10.1016/0887-8994(96)00083-5 ◽

1996 ◽

Vol 14 (4) ◽

pp. 317-321 ◽

Cited By ~ 17

Author(s):

Mana Kurihara ◽

Komei Kumagai ◽

Saburo Yagishita

Keyword(s):

Syndrome Type ◽

Sanfilippo Syndrome ◽

Autopsy Study ◽

Type C ◽

Term Survivor

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Primitive Neuroectodermal Tumor as the Second Malignant Neoplasm in a Long-term Survivor Child of Acute Lymphoblastic Leukemia: a Case Report

Chinese Medical Sciences Journal ◽

10.24920/003653 ◽

2020 ◽

Vol 0 (0) ◽

pp. 10

Author(s):

Keyword(s):

Case Report ◽

Acute Lymphoblastic Leukemia ◽

Primitive Neuroectodermal Tumor ◽

Lymphoblastic Leukemia ◽

Malignant Neoplasm ◽

Neuroectodermal Tumor ◽

Second Malignant Neoplasm ◽

Term Survivor

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Effect of EPSPS Gene Copy Number and Glyphosate Selection on Fitness of Glyphosate-Resistant Bassia Scoparia in The Field

10.21203/rs.3.rs-155310/v1 ◽

2021 ◽

Author(s):

Charlemagne Ajoc Lim ◽

Prashant Jha ◽

Vipan Kumar ◽

Alan T. Dyer

Keyword(s):

Sugar Beet ◽

Seed Production ◽

Great Plains ◽

Copy Number ◽

Gene Copy Number ◽

Reproductive Traits ◽

Gene Copy ◽

Epsps Gene ◽

Gene Copies

Abstract The widespread evolution of glyphosate-resistant (GR) Bassia scoparia in the U.S. Great Plains poses a serious threat to the long-term sustainability of GR sugar beet. Glyphosate resistance in B. scoparia is due to an increase in the EPSPS (5-enolpyruvyl-shikimate-3-phosphate) gene copy number. The variation in EPSPS gene copies among individuals from within a single GR B. scoparia population indicated a differential response to glyphosate selection. We tested the hypothesis of reduced GR B. scoparia fitness (reproductive traits) to increasing glyphosate rates (applied as single or sequential applications) potentially experienced within a GR sugar beet field. The variation in EPSPS gene copy number and total glyphosate rate (single or sequential applications) did not influence any of the reproductive traits of GR B. scoparia, except seed production. Sequential applications of glyphosate with a total rate of 2,214 g ae ha− 1 or higher prevented seed production in B. scoparia plants with 2–4 (low levels of resistance) and 5–6 (moderate levels of resistance) EPSPS gene copies. Timely sequential applications of glyphosate (full recommended rates) can potentially slow down the evolution of GR B. scoparia with low to moderate levels of resistance (2–6 EPSPS gene copies), but any survivors (highly-resistant individuals with ≥ 8 EPSPS gene copies) need to be mechanically removed before flowering from GR sugar beet fields. This research warrants the need to adopt ecologically based, multi-tactic strategies to reduce exposure of B. scoparia to glyphosate in GR sugar beet.

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Agro-economic substantiation of the feasibility of green manured fallow in sugar beet crop rotations of the Central Chernozem Region

BIO Web of Conferences ◽

10.1051/bioconf/20213202001 ◽

2021 ◽

Vol 32 ◽

pp. 02001

Author(s):

Alexander Akimenko ◽

Vyacheslav Sviridov ◽

Tatiana Dudkina

Keyword(s):

Sugar Beet ◽

Crop Rotation ◽

Crop Rotations ◽

Mineral Fertilizers ◽

Typical Chernozem ◽

Central Chernozem Region ◽

Sugar Beet Crop ◽

Absolute Decrease ◽

Central Chernozem

The aim of the study is to find out the feasibility of including green manured fallow in specialized sugar beet crop rotations by comparing necessary assessment indicators with their values in a traditional crop rotation with black fallow against the background of unequal fertilization levels. The study was based on the analysis of experimental data from a long-term stationary experiment, laid out simultaneously in all fields and variants on typical chernozem in triplicate. The results are as follows: within the same fertilization levels, no significant differences in the yield of winter wheat (sugar beet precursor) were revealed, and the yield of sugar beet in a crop rotation with green manured fallow was stably higher (no more than 5 to 7%), an increase in yield relative to the control fertilizer variant (6 tons of manure per hectare per year) against the background of a double rate of manure in combination with mineral fertilizers amounted to 21.7 to 23.4% for wheat and 14.3 to 15.6% for beets with an increase in the productivity of crop rotations (in natural and value terms) by 1.2 times, but an increase in costs by 1.3 times caused an absolute decrease in the level of profitability by 23 to 25%. Differences in the assessment indicators for specific fertilization variants increased over time due to an unequal degree of soil fertility reproduction, and therefore in the fifth cycle of the crop rotation with black fallow turned out to be 1.1 to 1.3 times greater than in the green manured rotation.

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Length of Efficacy for Control of Curly Top in Sugar Beet With Seed and Foliar Insecticides

Plant Disease ◽

10.1094/pdis-02-16-0142-re ◽

2016 ◽

Vol 100 (7) ◽

pp. 1364-1370 ◽

Cited By ~ 2

Author(s):

Carl A. Strausbaugh ◽

Erik J. Wenninger ◽

Imad A. Eujayl

Keyword(s):

Sugar Beet ◽

Host Resistance ◽

Seed Treatment ◽

Field Experiments ◽

Block Design ◽

Root Yield ◽

Pyrethroid Insecticides ◽

Beet Curly Top Virus ◽

Randomized Complete Block Design ◽

Untreated Check

Curly top in sugar beet caused by Beet curly top virus (BCTV) is an important yield-limiting disease that can be reduced via neonicotinoid and pyrethroid insecticides. The length of efficacy of these insecticides is poorly understood; therefore, field experiments were conducted with the seed treatment Poncho Beta (clothianidin at 60 g a.i. + beta-cyfluthrin at 8 g a.i. per 100,000 seed) and foliar treatment Asana (esfenvalerate at 55.48 g a.i./ha). A series of four experiments at different locations in the same field were conducted in 2014 and repeated in a neighboring field in 2015, with four treatments (untreated check, Poncho Beta, Asana, and Poncho Beta + Asana) which were arranged in a randomized complete block design with eight replications. To evaluate efficacy, viruliferous (contain BCTV strains) beet leafhoppers were released 8, 9, 10, or 11weeks after planting for each experiment, which corresponded to 1, 2, 3, and 4 weeks after Asana application. Over both years, in 30 of 32 observation dates for treatments with Poncho Beta and 14 of 16 observation dates for Asana, visual curly top ratings decreased an average of 41 and 24%, respectively, with insecticide treatments compared with the untreated check. Over both years, in eight of eight experiments for treatments with Poncho Beta and six of eight experiments for Asana, root yields increased an average of 39 and 32%, respectively, with treatment compared with the untreated check. Over both years, the Poncho Beta treatments increased estimated recoverable sucrose (ERS) yield by 75% compared with the untreated check for weeks 8 and 9. By week 10, only the Poncho Beta + Asana treatment led to increases in ERS in both years, while the influence of increasing host resistance may have made other treatments more difficult to separate. When considering curly top symptoms, root yield, and ERS among all weeks and years, there was a tendency for the insecticides in the Poncho Beta + Asana treatment to complement each other to improve efficacy.

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