scholarly journals Epithelial and neural cadherin expression in the mammalian reproductive tract and gametes and their participation in fertilization-related events

2015 ◽  
Vol 401 (1) ◽  
pp. 2-16 ◽  
Author(s):  
Mónica Hebe Vazquez-Levin ◽  
Clara Isabel Marín-Briggiler ◽  
Julieta Natalia Caballero ◽  
María Florencia Veiga
Author(s):  
Mai M. Said ◽  
Ramesh K. Nayak ◽  
Randall E. McCoy

Burgos and Wislocki described changes in the mucosa of the guinea pig uterus, cervix and vagina during the estrous cycle investigated by transmission electron microscopy. More recently, Moghissi and Reame reported the effects of progestational agents on the human female reproductive tract. They found drooping and shortening of cilia in norgestrel and norethindrone- treated endometria. To the best of our knowledge, no studies concerning the effects of mestranol and norethindrone given concurrently on the three-dimensional surface features on the uterine mucosa of the guinea pig have been reported. The purpose of this study was to determine the effect of mestranol and norethindrone on surface ultrastructure of guinea pig uterus by SEM.Seventy eight animals were used in this study. They were allocated into two groups. Group 1 (20 animals) was injected intramuscularly 0.1 ml vegetable oil and served as controls.


Author(s):  
R.P. Apkarian ◽  
J.S. Sanfilippo

The synthetic androgen danazol, is an isoxazol derivative of ethisterone. It is utilized in the treatment of endometriosis, fibrocystic breast disease, and has a potential use as a contraceptive. A study was designed to evaluate the ultrastructural changes associated with danazol therapy in a rat model. The preliminary investigation of the distal segment of the rat uterine horn was undertaken as part of a larger study intended to elucidate the effects of danazol on the female reproductive tract.Cross-sections (2-3 mm in length) of the distal segment of the uterine horn from sixteen Sprague-Dawley rats were prepared for SEM. Ten rats in estrus served as controls and six danazol treated rats were noted to have alterations of the estrus cycle i.e. a lag in cycle phase or noncycling patterns. Specimens were fixed in 3% glutaraldehyde in 0.05M phosphate buffer containing CaCl2 at pH 7.0-7.4 and chilled to 4°C. After a brief wash in distilled water, specimens were passed through a graded series of ethanol, critical point dryed in CO2 from absolute ethanol, and coated with 6nm Au. Observations were made with an IS1-40 SEM operated at 15kV.


Author(s):  
Lawrence M. Roth

The female reproductive tract may be the site of a wide variety of benign and malignant tumors, as well as non-neoplastic tumor-like conditions, most of which can be diagnosed by light microscopic examination including special stains and more recently immunoperoxidase techniques. Nevertheless there are situations where ultrastructural examination can contribute substantially to an accurate and specific diagnosis. It is my opinion that electron microscopy can be of greatest benefit and is most cost effective when applied in conjunction with other methodologies. Thus, I have developed an approach which has proved useful for me and may have benefit for others. In cases where it is deemed of potential value, glutaraldehyde-fixed material is obtained at the time of frozen section or otherwise at operation. Coordination with the gynecologic oncologist is required in the latter situation. This material is processed and blocked and is available if a future need arises.


Author(s):  
R. L. Reeder ◽  
S. H. Rogers ◽  
W. A. Shannon

Numerous morphological studies have dealt with the spermatheca of pulmonate gastropods. This globular organ, which is attached to the female portion of the reproductive tract by a long duct in these monoecious animals, has had various functions ascribed to it. Recent histochemical demonstrations of deoxyribonuclease, ribonuclease, protease, and acid phosphatase have provided, however, conclusive evidence that it is a digestive organ for the degradation of superfluous sperm and genital tract secretions. Only limited information concerning the spermatheca is available at the ultrastructural level, a fact providing the stimulus for the present study of this organ in Sonorella santaritana, a desert mountain snail from Arizona.


1970 ◽  
Vol 64 (3) ◽  
pp. 459-465 ◽  
Author(s):  
Ch. Owman ◽  
N.-O. Sjöberg ◽  
N. O. Sjöstrand ◽  
G. Swedin

ABSTRACT The effect of prolonged treatment with high doses of oestrogen and/or progesterone on the amount of adrenergic transmitter in the short adrenergic neurons of the male reproductive tract of castrated rats has been studied by chemical determinations and histochemical demonstration of noradrenaline. Oestrogen, progesterone, or a combination of both, had no overt effect on the total content or on the concentration of noradrenaline in the male genital organs. The results are discussed in the light of recent findings that the content of the noradrenaline transmitter in the short adrenergic neurons to the female genital tract is markedly influenced by these female sex hormones.


Reproduction ◽  
1973 ◽  
Vol 32 (2) ◽  
pp. 313-314
Author(s):  
P. Holst ◽  
P. Mattner ◽  
A. Braden
Keyword(s):  

Sign in / Sign up

Export Citation Format

Share Document