Somatostatin receptor subtype 1 and subtype 2 mRNA expression is regulated by nutritional state in rainbow trout (Oncorhynchus mykiss)

2004 ◽  
Vol 139 (3) ◽  
pp. 236-244 ◽  
Author(s):  
Barton J. Slagter ◽  
Jeffrey D. Kittilson ◽  
Mark A. Sheridan
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mrna Expression ◽  
Somatostatin Receptor ◽  
Nutritional State ◽  
Receptor Subtype ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Differential expression of two somatostatin receptor subtype 1 mRNAs in rainbow trout (Oncorhynchus mykiss)

2004 ◽  
Vol 32 (1) ◽  
pp. 165-177 ◽  
Author(s):  
BJ Slagter ◽  
MA Sheridan
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Somatostatin Receptor ◽  
The Other ◽  
Receptor Subtype ◽  
Signaling System ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Lower Intestine ◽  
Growth Development ◽  
Insight Into

Somatostatins (SSs) play important roles in the growth, development and metabolism of vertebrates. In this study, cDNAs for two unique somatostatin receptor variants were cloned and sequenced from rainbow trout. The two cDNAs, one consisting of 1755 bp and the other of 1743 bp, share 63.6% identity in nucleotide sequence and 94.1% identity in deduced amino acid sequence and presumably arose through gene duplication. Each cDNA encodes for a putative 371-amino acid somatostatin receptor (one designated sst1A and the other sst1B) containing seven transmembrane domains. Rainbow trout sst1A and sst1B have 64.4 and 65.5% similarity respectively with human sst1 and only 43-60% similarity with other subtypes. Trout sst1 mRNAs are differentially expressed, both in terms of distribution among tissues as well as in terms of abundance within selected tissues. Both sst1A and sst1B mRNAs were present in brain, stomach, liver, pancreas, upper and lower intestine, pyloric cecum, kidney and muscle, whereas only sst1B mRNA was present in the esophagus. sst1A mRNA was more abundant than sst1B in the optic tectum, whereas sst1B mRNA was more abundant than sst1A in liver. sst1A and sst1B mRNAs were equally abundant in pancreas. These findings contribute to the understanding of the evolution of the SS signaling system and provide insight into the mechanisms that regulate the expression of SS receptors.

scholarly journals 5-Hydroxytryptamine-induced calcium-channel gating in rainbow trout (Oncorhynchus mykiss) peripheral blood lymphocytes

1997 ◽  
Vol 323 (1) ◽  
pp. 251-258 ◽  
Author(s):  
François FERRIERE ◽  
Naïm A. KHAN ◽  
Jean P. MEYNIEL ◽  
Pierre DESCHAUX
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Peripheral Blood ◽  
Calcium Signalling ◽  
Peripheral Blood Lymphocytes ◽  
Free Calcium ◽  
Receptor Subtype ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Peripheral Blood Lympho

The present study was conducted on peripheral blood lympho-cytes of rainbow trout (Oncorhynchus mykiss) to assess the role of 5-hydroxytryptamine (5-HT; ‘serotonin’) in calcium signalling. 5-HT-induced increases in intracellular free calcium concentrations, [Ca2+]i, and its action was mediated by 5-HT receptor subtype 3 (5-HT3), but not by 5-HT receptor subtype 1A (5-HT1A) or subtype 2 (5-HT2) in these cells. In Ca2+-containing medium (1 mM CaCl2), 5-HT and 2-methyl-5-HT (5-HT3 receptor agonist) induced increases in [Ca2+]i, whereas in Ca2+-free medium (0 Ca2+, 1 mM EGTA), these two agents failed to evoke increases in [Ca2+]i in these cells, demonstrating that 5-HT mobilizes Ca2+ from the extracellular environment. Furthermore, 5-HT-induced increases in [Ca2+]i are not contributed to by the intracellular endoplasmic reticulum (ER) pool, as thapsigargin, an agent that recruits Ca2+ from ER stores, had additive effects on 5-HT-induced [Ca2+]i responses in fish peripheral lymphocytes. 5-HT-induced increases in [Ca2+]i were mediated by 5-HT3 receptors via gating the calcium through L-type, but not N-type, calcium channels in trout lymphocytes.

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