Correlation of serpin–protease expression by comparative analysis of real-time PCR profiling data

Introduction. Most of the bacteria exist in natural ecosystems not in the form of free floating cells; but in the form of biofilms attached to the substrate. One of the most ecologically important substrates is chitin. Vibrio cholerae; like most members of the Vibrionaceae family; has a chitinolytic complex and can degrade chitin. The ability of V. cholerae to form a biofilm on chitinous substrates can explain the mechanism of the formation of an ecological niche for the preservation and transfer of the pathogen to new regions with the likelihood of the formation of new foci of cholera.Aim — to determine the ability of V. cholerae to form a biofilm on the chitinous shell of crayfish (Astacus astacus) by means of real-time PCR.Materials and methods. A comparative analysis of the timing of biofilm formation by V. cholerae of different serogroups and toxigenicity was carried out.Results. In the course of the study; it was found that cholera vibrios were shown to be capable of forming a biofilm regardless the serogroup and toxigenicity. However; toxigenic tcpA+ strains have a higher intensity of biofilm formation than nontoxigenic ones; in which the tcpA gene is absent.

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Correction: Comparative Analysis of Gene Expression Level by Quantitative Real-Time PCR Has Limited Application in Objects with Different Morphology

PLoS ONE ◽

10.1371/annotation/fe0ad7f2-2921-4b13-903a-6eba19e8294b ◽

2012 ◽

Vol 7 (8) ◽

Cited By ~ 1

Author(s):

Natalia V. Demidenko ◽

Aleksey A. Penin

Keyword(s):

Gene Expression ◽

Comparative Analysis ◽

Real Time ◽

Real Time Pcr ◽

Gene Expression Level ◽

Expression Level ◽

Quantitative Real Time Pcr ◽

Limited Application

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PSVI-41 The comparative analysis of vaginal flora of high-productive cows in the postpartum period by Real-time PCR

Journal of Animal Science ◽

10.1093/jas/skz258.411 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 199-200

Author(s):

Elena A Korochkina ◽

Andrey Nechaev ◽

Anatoly Stekolnikov ◽

Anatoliy Nezhdanov

Keyword(s):

Comparative Analysis ◽

Real Time ◽

Real Time Pcr ◽

Postpartum Period ◽

Vaginal Discharge ◽

Bacterial Count ◽

Vaginal Flora ◽

High Count ◽

Yeast Fungi ◽

Apparently Healthy

Abstract The aim of the study was the comparative analysis of species composition of vaginal bugs of high-productive cows (n = 7) in the postpartum period by Real-time PCR. The material were vaginal discharge of cows (four of them had acute pyogenic endometritis, another was apparently healthy). The vaginal discharge of sick cows contained the low count of bacteria Megasphaera spp., Veillonella spp., Dialister sрp., Lactobacillus spp. and streptococcus. The evaluation of bacterial count of Lachnobacterium spp., Clostridium spp., actinomycetes Atopobium spp., bacteria Staphylococcus spp., and yeast fungi Candida spр. did not demonstrate statistically different between groups. Probably this bacteria were not the etiological origin of postpartum endometritis of cows. However, vaginal discharge of cows with diagnosis acute pyogenic endometritis had high count of bacteria of Fusobacteriaceae (6,0±3,8 and 5,3±2,6 lg genomes/g), Enterobacteriaceae (6,4±4,1 and 5,1±3,0 lg genomes/g), bacteroides of Prevotella spp. and Porphyromonas spp. (7,4±5,2 and 6,4±4,4 lg genomes/g), actinomycetes Mobiluncus spp. and Corynebacterium spp. (5,1±2,5 and 4,7±1,9 lg genomes/g), Peptostreptococcus spp. and Eubacterium spp., in comparison with apparently healthy cows. The presence of this bacteria in the vaginal discharge of apparently healthy cows suggestive that this bacteria are the permanent vaginal flora. Thus, comparative analysis of the dysbiotic sort of vaginal flora of cows in postpartum period demonstrated that vaginal discharge of cows with diagnosis acute pyogenic endometritis consists the high count of bacteria of potentially pathogenic and pathogenic groups (Fusobacteriaceae, Enterobacteriaceae, Prevotella spp., Porphyromonas spp., Mobiluncus spp., Peptostreptococcus spp., Eubacterium spp.).

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