scholarly journals Evaluation of the ability of cholera vibrios to form a biofilm on the surface of the chitinous shell of a crayfish by real-time PCR

2021 ◽  
Vol 98 (4) ◽  
pp. 434-439
Author(s):  
E. A. Menshikova ◽  
E. M. Kurbatova ◽  
S. O. Vodopyanov ◽  
R. V. Pisanov ◽  
S. V. Titova
Keyword(s):  
Comparative Analysis ◽  
Vibrio Cholerae ◽  
Real Time ◽  
Real Time Pcr ◽  
Biofilm Formation ◽  
Ecological Niche ◽  
Natural Ecosystems ◽  
Astacus Astacus

Introduction. Most of the bacteria exist in natural ecosystems not in the form of free floating cells; but in the form of biofilms attached to the substrate. One of the most ecologically important substrates is chitin. Vibrio cholerae; like most members of the Vibrionaceae family; has a chitinolytic complex and can degrade chitin. The ability of V. cholerae to form a biofilm on chitinous substrates can explain the mechanism of the formation of an ecological niche for the preservation and transfer of the pathogen to new regions with the likelihood of the formation of new foci of cholera.Aim — to determine the ability of V. cholerae to form a biofilm on the chitinous shell of crayfish (Astacus astacus) by means of real-time PCR.Materials and methods. A comparative analysis of the timing of biofilm formation by V. cholerae of different serogroups and toxigenicity was carried out.Results. In the course of the study; it was found that cholera vibrios were shown to be capable of forming a biofilm regardless the serogroup and toxigenicity. However; toxigenic tcpA+ strains have a higher intensity of biofilm formation than nontoxigenic ones; in which the tcpA gene is absent.

Comparative analysis of real-time PCR assays in the detection of canine visceral leishmaniasis

2018 ◽  
Vol 117 (10) ◽  
pp. 3341-3346 ◽  
Author(s):  
Juliana Barbosa Nunes ◽  
Wendel Coura-Vital ◽  
Fabio Antônio Colombo ◽  
Frederico José Moreira Baêta ◽  
Aimara Costa Pinheiro ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Visceral Leishmaniasis ◽  
Real Time ◽  
Real Time Pcr ◽  
Canine Visceral Leishmaniasis ◽  
Pcr Assays

scholarly journals Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns

2012 ◽  
Vol 7 (5) ◽  
pp. 829-838 ◽  
Author(s):  
Veronica Sanchez-Freire ◽  
Antje D Ebert ◽  
Tomer Kalisky ◽  
Stephen R Quake ◽  
Joseph C Wu
Keyword(s):  
Gene Expression ◽  
Comparative Analysis ◽  
Single Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Expression Patterns ◽  
Gene Expression Patterns

scholarly journals Correlation of serpin–protease expression by comparative analysis of real-time PCR profiling data

Genomics ◽  
2006 ◽  
Vol 88 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Sunita Badola ◽  
Heidi Spurling ◽  
Keith Robison ◽  
Eric R. Fedyk ◽  
Gary A. Silverman ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Protease Expression

scholarly journals Detection of Vibrio cholerae O1 and O139 in Environmental Water Samples by an Immunofluorescent-Aggregation Assay

2010 ◽  
Vol 76 (16) ◽  
pp. 5520-5525 ◽  
Author(s):  
Duochun Wang ◽  
Xuebin Xu ◽  
Xiaoling Deng ◽  
Changyi Chen ◽  
Baisheng Li ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Real Time ◽  
Water Samples ◽  
Real Time Pcr ◽  
High Specificity ◽  
Culture Method ◽  
Environmental Water ◽  
Estuarine Water ◽  
Conventional Culture ◽  
Conventional Culture Method

ABSTRACT Environmental waters are an important reservoir for Vibrio cholerae, and effective surveillance of the pathogen can help to warn of and prevent infection with this potentially fatal pathogen. An immunofluorescent-aggregation (IFAG) assay to detect V. cholerae O1 and O139 was established and evaluated with estuarine water samples. The practical application of this assay was compared with the conventional culture method and real-time PCR. The IFAG method had a sensitivity of 103 CFU/ml for detection of V. cholerae O1 and O139 strains in a suspension containing 10 different species of enterobacterial strains (total, 105 CFU/ml). Ten fluorescent bacterial aggregate colonies were randomly picked and tested positive in serum agglutination tests for the V. cholerae O1 and O139 strains, showing a high specificity. The enrichment broths of 146 samples of estuarine water were tested, and the percentage positive by the IFAG assay was 19.9% (29/146), which was significantly higher than that of the conventional culture method (10.3%, 15/146; P < 0.01) but lower than that of real-time PCR (29.5%, 43/146; P < 0.01). The coincidence rates of real-time PCR and IFAG detection were decreased with the reduction of the V. cholerae concentration. The IFAG method, with a high specificity and a relatively high sensitivity, may be used for detection and isolation of V. cholerae in environmental water samples.

A new real-time PCR assay for detecting fungi in genus Ceratocystis

Plant Disease ◽  
2021 ◽  
Author(s):  
Karthikeyan Dharmaraj ◽  
Alice Merrall ◽  
Julie A. Pattemore ◽  
Joanne Mackie ◽  
Brett J.R Alexander ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Plant Pathogens ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Stem Tissue ◽  
Natural Ecosystems ◽  
Pcr Assay ◽  
Practical Applications ◽  
Wide Range

The genus Ceratocystis contains several significant plant pathogens, causing wilt and canker disease on a wide range of plants species. Currently, there are over 40 known species of Ceratocystis, some of which are becoming increasingly important in agricultural or natural ecosystems. The diagnostics for most Ceratocystis species currently relies on time consuming and labour-intensive culturing approaches. To provide more time efficient and sensitive molecular diagnostic tools for Ceratocystis, a generic Taq-Man real-time PCR assay was developed using the ITS gene. This novel two-probe Taq-man assay amplified DNA from all tested Ceratocystis species. Some non-specific amplification of a few species from closely related genera was observed under certain conditions; however, these false positive detections could be ruled out using the additional PCR primers developed for further sequence based identification of the detected species. The assay was highly sensitive as it detected 0.2 pg/µl of Ceratocystis DNA in water as well as in host DNA matrix. Further validation with artificially inoculated fig stem tissue demonstrated that the assay was also able to effectively detect the pathogen in infected asymptomatic stem tissue. This newly developed real-time PCR assay has practical applications in biosecurity, conservation, and agriculture, enabling to detect Ceratocystis species directly from plant material, to facilitate more sensitive screening of imported plant germplasm, and allow rapid tracking of pathogens in case of disease outbreaks.

scholarly journals PSVI-41 The comparative analysis of vaginal flora of high-productive cows in the postpartum period by Real-time PCR

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 199-200
Author(s):  
Elena A Korochkina ◽  
Andrey Nechaev ◽  
Anatoly Stekolnikov ◽  
Anatoliy Nezhdanov
Keyword(s):  
Comparative Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Postpartum Period ◽  
Vaginal Discharge ◽  
Bacterial Count ◽  
Vaginal Flora ◽  
High Count ◽  
Yeast Fungi ◽  
Apparently Healthy

Abstract The aim of the study was the comparative analysis of species composition of vaginal bugs of high-productive cows (n = 7) in the postpartum period by Real-time PCR. The material were vaginal discharge of cows (four of them had acute pyogenic endometritis, another was apparently healthy). The vaginal discharge of sick cows contained the low count of bacteria Megasphaera spp., Veillonella spp., Dialister sрp., Lactobacillus spp. and streptococcus. The evaluation of bacterial count of Lachnobacterium spp., Clostridium spp., actinomycetes Atopobium spp., bacteria Staphylococcus spp., and yeast fungi Candida spр. did not demonstrate statistically different between groups. Probably this bacteria were not the etiological origin of postpartum endometritis of cows. However, vaginal discharge of cows with diagnosis acute pyogenic endometritis had high count of bacteria of Fusobacteriaceae (6,0±3,8 and 5,3±2,6 lg genomes/g), Enterobacteriaceae (6,4±4,1 and 5,1±3,0 lg genomes/g), bacteroides of Prevotella spp. and Porphyromonas spp. (7,4±5,2 and 6,4±4,4 lg genomes/g), actinomycetes Mobiluncus spp. and Corynebacterium spp. (5,1±2,5 and 4,7±1,9 lg genomes/g), Peptostreptococcus spp. and Eubacterium spp., in comparison with apparently healthy cows. The presence of this bacteria in the vaginal discharge of apparently healthy cows suggestive that this bacteria are the permanent vaginal flora. Thus, comparative analysis of the dysbiotic sort of vaginal flora of cows in postpartum period demonstrated that vaginal discharge of cows with diagnosis acute pyogenic endometritis consists the high count of bacteria of potentially pathogenic and pathogenic groups (Fusobacteriaceae, Enterobacteriaceae, Prevotella spp., Porphyromonas spp., Mobiluncus spp., Peptostreptococcus spp., Eubacterium spp.).

scholarly journals A Method for the Simultaneous Detection of Vibrio cholerae Strains and Drug-Resistant Genes in their Genome by Real-Time PCR

2018 ◽  
Vol 54 (9) ◽  
pp. 886-893
Author(s):  
A. A. Kritskii ◽  
N. B. Cheldyshova ◽  
S. P. Zadnova ◽  
N. A. Plekhanov ◽  
N. I. Smirnova
Keyword(s):  
Vibrio Cholerae ◽  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Drug Resistant ◽  
Resistant Genes
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