PSVI-41 The comparative analysis of vaginal flora of high-productive cows in the postpartum period by Real-time PCR

Abstract The aim of the study was the comparative analysis of species composition of vaginal bugs of high-productive cows (n = 7) in the postpartum period by Real-time PCR. The material were vaginal discharge of cows (four of them had acute pyogenic endometritis, another was apparently healthy). The vaginal discharge of sick cows contained the low count of bacteria Megasphaera spp., Veillonella spp., Dialister sрp., Lactobacillus spp. and streptococcus. The evaluation of bacterial count of Lachnobacterium spp., Clostridium spp., actinomycetes Atopobium spp., bacteria Staphylococcus spp., and yeast fungi Candida spр. did not demonstrate statistically different between groups. Probably this bacteria were not the etiological origin of postpartum endometritis of cows. However, vaginal discharge of cows with diagnosis acute pyogenic endometritis had high count of bacteria of Fusobacteriaceae (6,0±3,8 and 5,3±2,6 lg genomes/g), Enterobacteriaceae (6,4±4,1 and 5,1±3,0 lg genomes/g), bacteroides of Prevotella spp. and Porphyromonas spp. (7,4±5,2 and 6,4±4,4 lg genomes/g), actinomycetes Mobiluncus spp. and Corynebacterium spp. (5,1±2,5 and 4,7±1,9 lg genomes/g), Peptostreptococcus spp. and Eubacterium spp., in comparison with apparently healthy cows. The presence of this bacteria in the vaginal discharge of apparently healthy cows suggestive that this bacteria are the permanent vaginal flora. Thus, comparative analysis of the dysbiotic sort of vaginal flora of cows in postpartum period demonstrated that vaginal discharge of cows with diagnosis acute pyogenic endometritis consists the high count of bacteria of potentially pathogenic and pathogenic groups (Fusobacteriaceae, Enterobacteriaceae, Prevotella spp., Porphyromonas spp., Mobiluncus spp., Peptostreptococcus spp., Eubacterium spp.).

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Comparative analysis of real-time PCR assays in the detection of canine visceral leishmaniasis

Parasitology Research ◽

10.1007/s00436-018-6038-9 ◽

2018 ◽

Vol 117 (10) ◽

pp. 3341-3346 ◽

Cited By ~ 2

Author(s):

Juliana Barbosa Nunes ◽

Wendel Coura-Vital ◽

Fabio Antônio Colombo ◽

Frederico José Moreira Baêta ◽

Aimara Costa Pinheiro ◽

...

Keyword(s):

Comparative Analysis ◽

Visceral Leishmaniasis ◽

Real Time ◽

Real Time Pcr ◽

Canine Visceral Leishmaniasis ◽

Pcr Assays

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Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns

Nature Protocols ◽

10.1038/nprot.2012.021 ◽

2012 ◽

Vol 7 (5) ◽

pp. 829-838 ◽

Cited By ~ 119

Author(s):

Veronica Sanchez-Freire ◽

Antje D Ebert ◽

Tomer Kalisky ◽

Stephen R Quake ◽

Joseph C Wu

Keyword(s):

Gene Expression ◽

Comparative Analysis ◽

Single Cell ◽

Real Time ◽

Real Time Pcr ◽

Expression Patterns ◽

Gene Expression Patterns

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Correlation of serpin–protease expression by comparative analysis of real-time PCR profiling data

Genomics ◽

10.1016/j.ygeno.2006.03.017 ◽

2006 ◽

Vol 88 (2) ◽

pp. 173-184 ◽

Cited By ~ 11

Author(s):

Sunita Badola ◽

Heidi Spurling ◽

Keith Robison ◽

Eric R. Fedyk ◽

Gary A. Silverman ◽

...

Keyword(s):

Comparative Analysis ◽

Real Time ◽

Real Time Pcr ◽

Protease Expression

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Evaluation of the ability of cholera vibrios to form a biofilm on the surface of the chitinous shell of a crayfish by real-time PCR

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-99 ◽

2021 ◽

Vol 98 (4) ◽

pp. 434-439

Author(s):

E. A. Menshikova ◽

E. M. Kurbatova ◽

S. O. Vodopyanov ◽

R. V. Pisanov ◽

S. V. Titova

Keyword(s):

Comparative Analysis ◽

Vibrio Cholerae ◽

Real Time ◽

Real Time Pcr ◽

Biofilm Formation ◽

Ecological Niche ◽

Natural Ecosystems ◽

Astacus Astacus

Introduction. Most of the bacteria exist in natural ecosystems not in the form of free floating cells; but in the form of biofilms attached to the substrate. One of the most ecologically important substrates is chitin. Vibrio cholerae; like most members of the Vibrionaceae family; has a chitinolytic complex and can degrade chitin. The ability of V. cholerae to form a biofilm on chitinous substrates can explain the mechanism of the formation of an ecological niche for the preservation and transfer of the pathogen to new regions with the likelihood of the formation of new foci of cholera.Aim — to determine the ability of V. cholerae to form a biofilm on the chitinous shell of crayfish (Astacus astacus) by means of real-time PCR.Materials and methods. A comparative analysis of the timing of biofilm formation by V. cholerae of different serogroups and toxigenicity was carried out.Results. In the course of the study; it was found that cholera vibrios were shown to be capable of forming a biofilm regardless the serogroup and toxigenicity. However; toxigenic tcpA+ strains have a higher intensity of biofilm formation than nontoxigenic ones; in which the tcpA gene is absent.

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Comparison of diagnostics of bacterial vaginosis according to clinical signs with results of laboratory investigations

Journal of obstetrics and women s diseases ◽

10.17816/jowd65476-82 ◽

2016 ◽

Vol 65 (4) ◽

pp. 76-82

Author(s):

Elena V. Shipitsina ◽

Tatyana A. Khusnutdinova ◽

Olga S. Ryzhkova ◽

Anna A. Krysanova ◽

Olga V. Budilovskaya ◽

...

Keyword(s):

Bacterial Vaginosis ◽

Real Time ◽

Sensitivity And Specificity ◽

Real Time Pcr ◽

Vaginal Discharge ◽

Clinical Signs ◽

High Sensitivity ◽

Clinical Diagnostics ◽

Laboratory Diagnostics ◽

Molecular Method

Introduction. Bacterial vaginosis (BV) is associated with a number of reproductive health disorders, therefore timely and accurate diagnosis of this condition is exceedingly important. Objective.Comparison of effectiveness of clinical and laboratory diagnostics of BV in women with vaginal discharge. Material and methods. In total, 318 patients addressing gynecological clinics with complaints about vaginal discharge participated in the study. Clinical diagnostics of BV was performed in the clinics participating in patient enrollment in accordance with their clinical practice. For laboratory diagnostics, microscopy of Gram stained smears according to the Nugent method and quantitative real-time PCR were used. Sensitivity and specificity of clinical diagnostics of BV and the molecular method were evaluated using the Nugent method as reference standard. Results. With the Nugent method, BV was diagnosed in 27% of women, with real-time PCR — in 37% of women. Using clinical signs of BV, the condition was diagnosed in 91% women. Sensitivity and specificity of the real-time PCR were 97% and 87%, respectively. Sensitivity of clinical diagnostics was 100%, but specificity was only 17%. Conclusions. Diagnostics of BV based only on the presence of vaginal discharge leads to false positive results and requires laboratory confirmation. The molecular method has a high sensitivity and satisfactory specificity for BV diagnosis and can be used as an alternative to the Nugent method.

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