Total antioxidant capacity and oxidative damage to proteins and lipids in aged rat heart

2007 ◽  
Vol 42 (6) ◽  
pp. S117-S118 ◽  
Author(s):  
Peter Kaplan ◽  
Monika Sivonova ◽  
Zuzana Tatarkova ◽  
Eva Babusikova ◽  
Jan Lehotsky ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Rat Heart ◽  
Aged Rat ◽  
Total Antioxidant ◽  
Oxidative Damage To Proteins

scholarly journals Placental Adaptive Changes to Protect Function and Decrease Oxidative Damage in Metabolically Healthy Maternal Obesity

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 794 ◽  
Author(s):  
Celeste Santos-Rosendo ◽  
Fernando Bugatto ◽  
Alvaro González-Domínguez ◽  
Alfonso M. Lechuga-Sancho ◽  
Rosa Maria Mateos ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Nitrosative Stress ◽  
Antioxidant Response ◽  
Placental Function ◽  
Total Antioxidant ◽  
Metabolically Healthy ◽  
Inducible Nitric Oxide

Pregnancy-related disorders, including preeclampsia and gestational diabetes, are characterized by the presence of an adverse intrauterine milieu that may ultimately result in oxidative and nitrosative stress. This scenario may trigger uncontrolled production of reactive oxygen species (ROS) such as superoxide anion (O●−) and reactive nitrogen species (RNS) such as nitric oxide (NO), along with an inactivation of antioxidant systems, which are associated with the occurrence of relevant changes in placental function through recognized redox post-translational modifications in key proteins. The general objective of this study was to assess the impact of a maternal obesogenic enviroment on the regulation of the placental nitroso-redox balance at the end of pregnancy. We measured oxidative damage markers—thiobarbituric acid-reacting substances (TBARS) and carbonyl groups (C=O) levels; nitrosative stress markers—inducible nitric oxide synthase, nitrosothiol groups, and nitrotyrosine residues levels; and the antioxidant biomarkers—catalase and superoxide dismutase (SOD) activity and expression, and total antioxidant capacity (TAC), in full-term placental villous from both pre-pregnancy normal weight and obese women, and with absence of metabolic complications throughout gestation. The results showed a decrease in C=O and TBARS levels in obese pregnancies. Although total SOD and catalase concentrations were shown to be increased, both activities were significantly downregulated in obese pregnancies, along with total antioxidant capacity. Inducible nitric oxide sintase levels were increased in the obese group compared to the lean group, accompanied by an increase in nitrotyrosine residues levels and lower levels of nitrosothiol groups in proteins such as ERK1/2. These findings reveal a reduction in oxidative damage, accompanied by a decline in antioxidant response, and an increase via NO-mediated nitrative stress in placental tissue from metabolically healthy pregnancies with obesity. All this plausibly points to a placental adaptation of the affected antioxidant response towards a NO-induced alternative pathway, through changes in the ROS/RNS balance, in order to reduce oxidative damage and preserve placental function in pregnancy.

scholarly journals Effects of Regular Treadmill Exercise on a DNA Oxidative-Damage Marker and Total Antioxidant Capacity in Rat Hippocampal Tissue

2016 ◽  
Vol 12 (4) ◽  
pp. 414 ◽  
Author(s):  
Soleiman Mahjoub ◽  
Arezoo Ghadi ◽  
Roghayeh Pourbagher ◽  
Karimollah Hajian-Tilaki ◽  
Jila Masrour-Roudsari
Keyword(s):  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Treadmill Exercise ◽  
Dna Oxidative Damage ◽  
Hippocampal Tissue ◽  
Total Antioxidant ◽  
Damage Marker

Effect of vitamin E and C supplementation on oxidative damage and total antioxidant capacity in lead-exposed workers

2014 ◽  
Vol 37 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Adela-Leonor Rendón-Ramírez ◽  
María Maldonado-Vega ◽  
Martha-Angelica Quintanar-Escorza ◽  
Gerardo Hernández ◽  
Bertha-Isabel Arévalo-Rivas ◽  
...  
Keyword(s):  
Vitamin E ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Exposed Workers ◽  
Total Antioxidant ◽  
Vitamin E And C

Total antioxidant capacity is impaired in different structures from aged rat brain

2005 ◽  
Vol 23 (8) ◽  
pp. 663-671 ◽  
Author(s):  
Ionara Rodrigues Siqueira ◽  
Cíntia Fochesatto ◽  
Aline Andrade ◽  
Melissa Santos ◽  
Martine Hagen ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Rat Brain ◽  
Total Antioxidant Capacity ◽  
Aged Rat ◽  
Total Antioxidant

scholarly journals Gender influence on antioxidant capacity and oxidative damage in saliva of children with autism spectrum disorder: a preliminary study

2021 ◽  
Vol 50 ◽  
Author(s):  
Matheus Morcela De SOUZA ◽  
João Victor Soares RODRIGUES ◽  
Maria Eduarda CABRERIZO GONÇALVES ◽  
Ana Carolina Polanowski ROSSATO ◽  
Maria Cristina Ruiz VOMS STEIN ◽  
...  
Keyword(s):  
Autism Spectrum Disorder ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Children With Autism ◽  
Autism Spectrum ◽  
Spectrum Disorder ◽  
Gender Influence ◽  
Total Antioxidant ◽  
Preliminary Study

Abstract Introduction Autism is a complex neurological disorder with multifactorial causes, which compromises physical, psychological, emotional and social skills. This disorder also impairs dental care and patient oral health. Saliva can be easily obtained using a non-invasive technique and has been used in the diagnosis and screening of many pathologies. Total antioxidant capacity of saliva is reduced in the presence of caries in children and has been studied as a possible marker for autism. However, data on salivary antioxidants in children with autism spectrum disorder (ASD) is scarce. Objective This preliminary study evaluated the existence of gender influence on non-enzymatic antioxidant capacity and protein oxidative damage in the saliva of children with this disorder. Our hypothesis is that there are gender differences in these biochemical parameters in children with austim. Material and method Whole saliva samples were collected by aspiration between 8:00-11:00 am from 12 girls and 12 boys aged 5 to 15 years, with previous diagnosis for this disorder. Total antioxidant capacity, uric acid, protein concentration and oxidative damage to proteins were analyzed by spectrophotometry. Values were presented as mean ± standard deviation and tested for adherence to normality (Shapiro-Wilk test). The data were then compared using the Mann-Whitney test, considering a 5% significance level. Result There was no significant difference between groups for all analyzed parameters (P>0.05). Conclusion Based on the results obtained, we concluded that gender does not influence the levels of oxidative stress markers in the saliva of children with ASD.

Antioxidative activity of selected fruits and vegetables

Biologia ◽  
2006 ◽  
Vol 61 (3) ◽  
Author(s):  
Ingrid Žitňanová ◽  
Silvia Ranostajová ◽  
Hana Sobotová ◽  
Denisa Demelová ◽  
Ivan Pecháň ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Fruits And Vegetables ◽  
Direct Methods ◽  
Inhibitory Effect ◽  
Green Beans ◽  
Age Related ◽  
Total Antioxidant ◽  
Oxidative Damage To Proteins

AbstractRecent studies have demonstrated that dietary plants are rich source of antioxidants and can contribute to the protection from age-related diseases. The aim of our study was to determine the total antioxidant capacity of extracts from different kinds of fruits and vegetables, and to examine their inhibitory effect on the oxidative damage to proteins in vitro. For determination of antioxidant capacity we used two direct methods. Among the food materials chosen for the present study, blueberries and red beets gave the maximum antioxidant activity. The lowest activity was determined in pears and green beans. Some extracts were more active in one method, while their activity was lower using the other method. To investigate inhibitory effects of fruits and vegetables extracts on the oxidative damage to proteins in vitro, we induced the oxidative damage to plasma proteins by sodium hypochlorite leading to formation of carbonyl compounds detected by spectrophotometric method. All extracts of fruits and vegetables showed inhibitory activity on the oxidative damage to proteins with raspberries and leek as most effective. Results of this study will be useful as an aid for dietary choices to increase antioxidant intake and will allow the investigation of the relation between dietary antioxidants and oxidative stress-induced diseases.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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