scholarly journals Simultaneous Sequencing of Multiple Polymerase Chain Reaction Products and Combined Polymerase Chain Reaction with Cycle Sequencing in Single Reactions

2002 ◽  
Vol 161 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Kathleen M. Murphy ◽  
James R. Eshleman
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Cycle Sequencing ◽  
Polymerase Chain ◽  
Multiple Polymerase Chain Reaction

Audiologic Features of Hearing Loss Due to the 1,555 Mutation of Mitochondrial DNA

1997 ◽  
Vol 106 (8) ◽  
pp. 643-648 ◽  
Author(s):  
Takashi Tsuiki ◽  
Kazuo Murai ◽  
Ken Kitamura ◽  
Seiko Murai ◽  
Yuya Tamagawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Hearing Loss ◽  
Mitochondrial Dna ◽  
Direct Sequencing ◽  
High Tone ◽  
Reaction Products ◽  
Chain Reaction ◽  
Cycle Sequencing ◽  
Sequencing Method ◽  
Polymerase Chain

We proved a 1,555 mutation of mitochondrial DNA in one member of each of three families with familial streptomycin hearing loss, and report the pedigrees and audiologic features. DNA was extracted by the standard method. The 1,555 A to G mutation was identified in all three patients and confirmed by direct sequencing of the polymerase chain reaction products by a cycle sequencing method. On audiograms, the hearing loss was sensorineural, bilateral, and symmetric, showing a high-tone loss or a profound loss particularly in the high-tone range, and the “symmetry law” of Langenbeck was applicable. The superimposed audiograms of members of one family did not cross themselves, proving the applicability of the “never-cross principle of audiograms.”

One-Step Directional Cloning of Blunt-Ended Polymerase Chain Reaction Products

2000 ◽  
Vol 287 (2) ◽  
pp. 349-352 ◽  
Author(s):  
Vincent W.S. Liu ◽  
Phillip Nagley ◽  
Hextan Y.S. Ngan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Directional Cloning ◽  
One Step ◽  
Polymerase Chain
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