Protein surface mapping by chemical oxidation: Structural analysis by mass spectrometry

Individual fructan tri-, tetra- and pentasaccharide isomers in neutral, water-soluble extracts from Lolium temulentum were purified and the linkages present in these isomeric oligosaccharides were analysed by combined GC-mass spectrometry of partially methylated alditol acetates. 1-Kestose and neokestose were the most abundant trisaccharides with 6-kestose present in much lower amounts. Analysis of isomers of DP 4 and 5 showed that multiple linkage types were present with structures based on all three trisaccharides. Oligosaccharides based on neokestose but with 2,6 linkages between adjacent fructose residues have not been previously detected in higher plants. © 1992.

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Faculty Opinions recommendation of Structural analysis of a prokaryotic ribosome using a novel amidinating cross-linker and mass spectrometry.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718039800.793480531 ◽

2013 ◽

Author(s):

Laura Trinkle-Mulcahy

Keyword(s):

Mass Spectrometry ◽

Structural Analysis ◽

Cross Linker

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Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

Journal of Proteome Research ◽

10.1021/pr034030n ◽

2003 ◽

Vol 2 (6) ◽

pp. 581-588 ◽

Cited By ~ 19

Author(s):

Kristina Håkansson ◽

Mark R. Emmett ◽

Alan G. Marshall ◽

Pia Davidsson ◽

Carol L. Nilsson

Keyword(s):

Mass Spectrometry ◽

Cerebrospinal Fluid ◽

Structural Analysis ◽

High Resolution ◽

High Resolution Mass Spectrometry ◽

Human Cerebrospinal Fluid ◽

High Resolution Mass ◽

2D Gel ◽

Resolution Mass

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Two New Phomaligols from the Marine-Derived Fungus Aspergillus flocculosus and Their Anti-Neuroinflammatory Activity in BV-2 Microglial Cells

Marine Drugs ◽

10.3390/md19020065 ◽

2021 ◽

Vol 19 (2) ◽

pp. 65

Author(s):

Byeoung-Kyu Choi ◽

Duk-Yeon Cho ◽

Dong-Kug Choi ◽

Phan Thi Hoai Trinh ◽

Hee Jae Shin

Keyword(s):

Nitric Oxide ◽

Mass Spectrometry ◽

Optical Rotation ◽

Chemical Oxidation ◽

Culture Broth ◽

Membered Ring ◽

Microglial Cells ◽

No Production ◽

Nmr Data ◽

Ic50 Value

Two new phomaligols, deketo-phomaligol A (1) and phomaligol E (2), together with six known compounds (3–8) were isolated from the culture broth of the marine-derived fungus Aspergillus flocculosus. Compound 1 was first isolated as a phomaligol derivative possessing a five-membered ring. The structures and absolute configurations of the new phomaligols were determined by detailed analyses of mass spectrometry (MS), nuclear magnetic resonance (NMR) data, optical rotation values and electronic circular dichroism (ECD). In addition, the absolute configurations of the known compounds 3 and 4 were confirmed by chemical oxidation and comparison of optical rotation values. Isolated compounds at a concentration of 100 μM were screened for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. Among the compounds, 4 showed moderate anti-neuroinflammatory effects with an IC50 value of 56.6 μM by suppressing the production of pro-inflammatory mediators in activated microglial cells without cytotoxicity.

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