Involvement of oxygen radicals in cytarabine-induced apoptosis in human polymorphonuclear cells11Abbreviations: ROS, reactive oxygen species; PMNs, polymorphonuclear neutrophils; SOD, superoxide dismutase; Ara-C, cytarabine; Ara-CTP, cytarabine 5′-triphosphate; O2−, superoxide anion; H2O2, hydrogen peroxide; f-MLP, N-Formyl-Met-Leu-Phe; PMA, phorbol 12-myristate 13-acetate; cyt c, cytochrome c; H7, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine; MTT, (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; thiazolyl blue); MoAb, monoclonal antibody; NAC, N-acetyl-l-cysteine; DCFH-DA, 2′,7′-dichlorofluorescin diacetate; DCF, 2′,7′-dichlorofluorescein; DCFH, dichlorofluorescin; and DPI, diphenyleneiodonium chloride.

2001 ◽  
Vol 61 (8) ◽  
pp. 1033-1040 ◽  
Author(s):  
Metello Iacobini ◽  
Adriana Menichelli ◽  
Giuseppe Palumbo ◽  
Giuseppe Multari ◽  
Beate Werner ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Anion ◽  
Oxygen Radicals ◽  
Polymorphonuclear Neutrophils ◽  
Oxygen Species ◽  
Diphenyltetrazolium Bromide ◽  
Dichlorofluorescin Diacetate ◽  
Cyt C ◽  
Induced Apoptosis ◽  
Ros Reactive Oxygen Species

scholarly journals Clinical Isolates ofMycobacterium tuberculosisDiffer in Their Ability to Induce Respiratory Burst and Apoptosis in Neutrophils as a Possible Mechanism of Immune Escape

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
María M. Romero ◽  
Luciana Balboa ◽  
Juan I. Basile ◽  
Beatriz López ◽  
Viviana Ritacco ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reference Strain ◽  
Immune Escape ◽  
Multidrug Resistant ◽  
Polymorphonuclear Neutrophils ◽  
Ros Production ◽  
Oxygen Species ◽  
Phenotypic Differences ◽  
Induced Apoptosis ◽  
P38 Pathway

Tuberculosis pathogenesis was earlier thought to be mainly related to the host but now it appears to be clear that bacterial factors are also involved. Genetic variability ofMycobacterium tuberculosis(Mtb) could be slight but it may lead to sharp phenotypic differences. We have previously reported that nonopsonizedMtbH37Rv induce apoptosis of polymorphonuclear neutrophils (PMNs) by a mechanism that involves the p38 pathway. Here we evaluated the capability to induce PMN apoptosis of two prevalentMtblineages in Argentina, the Latin America and Mediterranean (LAM), and Haarlem, using the H37Rv as a reference strain. Results showed that LAM strains strongly induced apoptosis of PMN which correlated with the induction of reactive oxygen species (ROS) production and p38 activation. Interestingly, the highly prosperous multidrug-resistant M strain, belonging to the Haarlem lineage, lacked the ability to activate and to induce PMN apoptosis as a consequence of (1) a weak ROS production and (2) the contribution of antiapoptotic mechanisms mediated at least by ERK. Although with less skill, M is able to enter the PMN so that phenotypic differences could lead PMN to be a reservoir allowing some pathogens to prevail and persist over other strains in the community.

Danshensu Rescues Ischemia/Reperfusion Caused Human Hepatocyte Death

2018 ◽  
Vol 17 (2) ◽  
pp. 117-121
Author(s):  
Sun Maw-Sheng ◽  
Liang Chun-Ya ◽  
Hsieh Po-Chun ◽  
Kuo Chan-Yen
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Caspase 3 ◽  
Ischemia Reperfusion ◽  
Oxygen Species ◽  
Good Strategy ◽  
Ros Accumulation ◽  
Dichlorofluorescin Diacetate ◽  
Hepatocyte Damage ◽  
Hepatocyte Death

Apoptosis of hepatocyte, under ischemia/reperfusion (IR) conditions, has been identified as an essential process in the progression of liver transplantation. Under these conditions, mitochondria can become a threat to the cell because of their capacity to generate reactive oxygen species (ROS). Additionally, ROS overproduction may induce inflammation. As ROS accumulation appears to cause hepatocyte damage or death, there has been considerable interest in identifying the candidate natural products involved and in developing strategies to reduce oxidative stress. In this study, we use Danshensu as a candidate product to speculate whether has the protective effect on apoptotic hepatocyte upon IR. To speculate the apoptotic phenomena was reversed by Danshensu, we detected the p53, cleaved-caspase 3 expression by western blotting, as well as caspase-3 activity. Additionally, we analyzed the ROS levels by 2′,7′-dichlorofluorescin diacetate (DCF-DA) staining. We also detected the cell viability by WST-1. Results showed that Danshensu alleviated hypoxia-caused cell apoptosis via ROS overproduction. We suggested that Danshensu is a good strategy for treating hepatocyte damage upon IR.

scholarly journals A Novel Tetrapeptide Derivative Exhibits In Vitro Inhibition of Neutrophil-Derived Reactive Oxygen Species and Lysosomal Enzymes Release

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Sumitra Miriyala ◽  
Manikandan Panchatcharam ◽  
Meera Ramanujam ◽  
Rengarajulu Puvanakrishnan
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Anion ◽  
Lysosomal Enzymes ◽  
Reactive Oxygen ◽  
Peptide Sequence ◽  
Ros Generation ◽  
Oxygen Species ◽  
Complement Factors

Neutrophil infiltration plays a major role in the pathogenesis of myocardial injury. Oxidative injury is suggested to be a central mechanism of the cellular damage after acute myocardial infarction. This study is pertained to the prognostic role of a tetrapeptide derivative PEP1261 (BOC-Lys(BOC)-Arg-Asp-Ser(tBu)-OtBU), a peptide sequence (39–42) of lactoferrin, studied in the modulation of neutrophil functions in vitro by measuring the reactive oxygen species (ROS) generation, lysosomal enzymes release, and enhanced expression of C proteins. The groundwork experimentation was concerned with the isolation of neutrophils from the normal and acute myocardial infarct rats to find out the efficacy of PEP1261 in the presence of a powerful neutrophil stimulant, phorbol 12-myristate 13 acetate (PMA). Stimulation of neutrophils with PMA resulted in an oxidative burst of superoxide anion and enhanced release of lysosomal enzymes and expression of complement proteins. The present study further demonstrated that the free radicals increase the complement factors in the neutrophils confirming the role of ROS. PEP1261 treatment significantly reduced the levels of superoxide anion and inhibited the release of lysosomal enzymes in the stimulated control and infarct rat neutrophils. This study demonstrated that PEP1261 significantly inhibited the effect on the ROS generation as well as the mRNA synthesis and expression of the complement factors in neutrophils isolated from infarct heart.

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