scholarly journals A tethered adhesive particle model of two-dimensional elasticity and its application to the erythrocyte membrane

1996 ◽  
Vol 70 (2) ◽  
pp. 857-867 ◽  
Author(s):  
S. Feng ◽  
R.C. MacDonald
1982 ◽  
Vol 28 (4) ◽  
pp. 925-931 ◽  
Author(s):  
B B Rosenblum ◽  
S M Hanash ◽  
N Yew ◽  
J V Neel

Abstract In an effort to maximize the amount of genetic information that can be extracted from a blood sample, we investigated the use of two-dimensional polyacrylamide-gel electrophoresis (PAGE) to resolve the protein constituents of the erythrocyte membrane. Lyophilized membranes were dissolved in various concentrations of urea, NP-40 detergent, and mercaptoethanol and subjected to two-dimensional PAGE by a modification of the O'Farrell procedure, with use of the ISO-DALT apparatus. More than 600 spots were visible in silver-stained gels under conditions that excluded specific cytoskeleton protein components, including spectrin and actin. The reproducibility of the pattern depended highly on the precise composition of the solubilization mixture. Poor resolution was observed in the presence of actin and other proteins of high molecular mass (spectrin bands 1 and 2) when we used high urea concentrations that solubilized the entire erythrocyte membrane. The large number of polypeptides observed could not be attributed to proteolysis, because addition of proteolytic inhibitors to the membrane wash solutions did not alter the pattern on the gel. The pattern also did not appear to include erythrocyte cytosol proteins because, except for globin, none of five purified erythrocyte lysate proteins was visible in the erythrocyte membrane gels. We conclude that two-dimensional electrophoresis provides a powerful tool for the study of non-cytoskeletal erythrocyte membrane proteins.


Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2169 ◽  
Author(s):  
Zhenyu Wang ◽  
Lin Wang ◽  
Wengang Zhang

Generation of particles with irregular shape and the overlap detection are crucial for numerical simulation of granular materials. This paper presents a systematic approach to develop a two-dimensional random particle model for numerical simulation of granular materials. Firstly, a random angular bend (RAB) algorithm is proposed and coded in Python to simulate the geometric model of individual particle with irregular shape. Three representative parameters are used to quantitatively control the shape feature of generated polygons in terms of three major aspects, respectively. Then, the generated geometrical models are implemented into particle flow code PFC2D to construct the clump library. The clumps are created via the mid-surface method. Besides, an overlap detection algorithm is developed to address the difficulties associated with spatial allocation of irregularly shaped particles. Finally, two application examples are adopted to validate the feasibility of the proposed algorithm in the numerical modeling of realistic granular materials. The study provides a solid foundation for the generation and simulation of the granular materials based on angular bend theory.


1975 ◽  
Vol 394 (4) ◽  
pp. 550-557 ◽  
Author(s):  
Sucharit Bhakdi ◽  
Hubertus Knüfermann ◽  
Donald F.Hoelzl Wallach

2004 ◽  
Vol 108 (1089) ◽  
pp. 585-592 ◽  
Author(s):  
A. Panaras ◽  
S. G. Voutsinas

AbstractA two-dimensional vortex particle model is used for studying the development of the vortex wake generated by the wing of an aircraft in the Trefftz plane. Two-dimensional, finite-area vortex structures simulate the initial vorticity distribution at a near-field cross-section of the wing, as provided by either measurements or simulations. The code is used for studying the effect of weak or strong counter-rotating vortices on the development of an aircraft wake. Application in a three-vortex configuration, consisting of the tip and flap vortices, plus a weak negative vorticity sheet lying between them, supports the hypothesis of Graham that the merging of the tip and flap vortices is prevented, because in such an arrangement the counter-rotating vorticity sheet is wrapped around the primary vortices. The present results indicate that even in the far field the tip and flap vortices remain distinct and follow a helical trajectory with large-pitch and small-radius. The code is next applied to the two-vortex system of Savas’s triangular wing, in which the circulation of the flap vortex is comparable to that of the tip. Although a 2D analysis is inappropriate for stability analysis it is still useful for a quick qualitative investigation. Results indicate that the flap and tip vortices follow a helical trajectory with large-pitch but also very large radius. During one period, the flap vortex covers a span wise distance equal to the wing span. Such a flow has not been observed in flight or in laboratory tests. Actually, Ortegaet al, who studied experimentally the triangular wing of O. Savas, found out that before concluding one full spiral, the vortices are literally destroyed (rapid spreading of their vorticity) by an instability mechanism.


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