The action of “low potency” homœopathic remedies on the movement of guinea-pig macrophages and human leucocytes

1982 ◽  
Vol 71 (02) ◽  
pp. 48-61 ◽  
Author(s):  
Victor A. Moss ◽  
J. Alan Roberts ◽  
Keith Simpson
Keyword(s):  
Guinea Pig ◽  
Human Subject ◽  
Human Subjects ◽  
Active Agent ◽  
Cell Movement ◽  
Source Material ◽  
Significant Modification ◽  
The Public ◽  
Series Of Experiments

SummaryThe action of five “low potency” homœopathic remedies, as supplied to the public and used clinically, on the distance of movement in a given time of guinea-pig macrophages and human leucocytes was measured. The remedies, Belladonna, Hepar sulphur, Pyrogenium, Silicea and Staphylococcin, were tested in the range 2×10−10 to 10−16 g/ml dilution of the source material, though the actual concentration of any active agent was probably considerably less than these values.In four series of experiments with guinea-pig macrophages the remedies were tested as aqueous or alcoholic tinctures, or absorbed on sugar granules. The results of 533 tests showed statistically significant modification of cell movement in 47 tests (i.e. 95% significant in about 8% of tests). Ten of these differences were significant at the 99% level and three also at the 99.9% level.Fifty tests of the aqueous tinctures on leucocytes from four human subjects showed modification of cell movement in five tests, one test being significant at the 99% level. Significant effects were obtained with two subjects only.The remedies facilitated movement in some experiments and inhibited it in others. The largest effects were obtained with a few sensitive guinea-pigs and one human subject. The magnitude and direction of the effects depended on the batch of animals being tested rather than on the set of remedies used.It is concluded that low potency homœopathic remedies prepared by A. Nelson & Company Ltd. are capable of modifying the movement of human leucocytes and guinea-pig macrophages in vitro.

Progressive Increase in Platelet Activation With Smoke Exposure: Human Subject and In-Vitro Studies

2007 ◽  
Author(s):  
Gaurav Girdhar ◽  
Sulan Xu ◽  
Jolyon Jesty ◽  
Danny Bluestein
Keyword(s):  
Cigarette Smoke ◽  
Platelet Activation ◽  
Human Subject ◽  
Prolonged Exposure ◽  
Human Subjects ◽  
Progressive Increase ◽  
In Vitro Studies ◽  
Protective Effects ◽  
Shs Exposure

Second hand cigarette smoke (SHS) is one of the major risk factors for cardiovascular disease (CVD) and has been shown to substantiate platelet activation and aggregation in several studies [1, 2]. Most of these studies, under chronic or acute exposure conditions or over prolonged exposure, do not represent the initiation of a disease state or hematological damage under normal levels of cigarette smoke. These above studies of platelet activation with SHS together with our previous in-vitro studies demonstrating cardio-protective effects of nicotine [3], have motivated the present investigation of physiological levels of SHS exposure on human subjects and within an in-vitro endothelial cell-platelet system, with cigarettes (or smoke extracts) of varying nicotine content to confirm analogous cardio-protective effects of nicotine.

scholarly journals Conditions of Legality of Medical Human Subject Research

Medicne pravo ◽  
2021 ◽  
pp. 69-77
Author(s):  
M. V. Mendzhul
Keyword(s):  
Human Rights ◽  
Medical Research ◽  
Human Genome ◽  
Human Subject ◽  
Human Subjects ◽  
Research Process ◽  
Embryo Research ◽  
Human Subject Research ◽  
Nuremberg Code

The article examines international acts and national legislation and highlights the conditions for the legality of medical research with human participation. The provisions of the Nuremberg Code (1947), the Helsinki Declaration of the World Medical Association «Ethical principles for medical research involv- ing human subjects» (1964), the Universal Declaration on the Human Genome and Human Rights (1997), the Convention for the Protection of Human Rights and Dignity of the Human Being with regard to the Application of Biology and Medicine: Convention on Human Rights and Biomedicine (1997) and the Addi- tional Protocols to the Convention, Regulation of the European Parliament and of the Council (EU) No 536/2014. The support of the recommendation to ratify the Convention on Human Rights and Biomedicine and its additional protocols in Ukraine has been expressed. It has been established that international acts stipulate that the design and implementation of each human subject research must be clearly described in the research protocol. In addition, research protocols must be subject to prior review by the Ethics Committees. It has been substantiated that international acts set for medical research standards, which are based on the principles of respect for dignity and human rights, the priority of interests of the person over the interests of society or sci- ence, compliance with safety requirements and prevention of harm to humans, mandatory permission to conduct medical examination, research and control- lability of the research process and its results, compensation for any damage caused by medical research. Conditions of legality of medical research can be divided into general (obser- vance of which is always necessary if a person participates in experiments) and special (additional conditions of legality, which are put forward depend- ing on the field or object of research, namely in the field of experiments com- bined with medical care , human genome research, in vitro embryo research, in the field of clinical trials of drugs).

scholarly journals Why the Readiness Potential Does Not Disprove Free Will

2021 ◽  
Vol 14 (1) ◽  
pp. 125-133
Author(s):  
Even Totland
Keyword(s):  
Free Will ◽  
Human Subject ◽  
Human Subjects ◽  
Readiness Potential ◽  
Neural Processes ◽  
Series Of Experiments ◽  
The Brain

Neuroscientist Benjamin Libet has conducted a series of experiments that reveal the existence of certain neural processes in the brain of human subjects, initiating an action prior to the human subject’s intention to act, thus seemingly threatening our idea of free will. The purpose of this paper is to show how these processes do not disprove any idea of free will one might have as one would, if accepting such a thesis, be committing two distinct mereological fallacies and ultimately, would treat the human subject as inhabiting some of its parts as opposed to being the sum of its parts.

Clonidine and Dexmedetomidine Potently Inhibit Peristalsis in the Guinea Pig Ileum In Vitro 

2002 ◽  
Vol 97 (6) ◽  
pp. 1491-1499 ◽  
Author(s):  
Michael K. Herbert ◽  
Susanne Roth-Goldbrunner ◽  
Peter Holzer ◽  
Norbert Roewer
Keyword(s):  
Guinea Pig ◽  
Drug Effects ◽  
In Vitro Study ◽  
Intraluminal Pressure ◽  
Organ Bath ◽  
Guinea Pig Ileum ◽  
Pressure Threshold ◽  
Intestinal Peristalsis ◽  
Series Of Experiments

Background Inhibition of intestinal peristalsis is a major side effect of drugs used for anesthesia or for analgesia and sedation of patients in the intensive care unit. This in vitro study examined the effect of clonidine and dexmedetomidine on intestinal peristalsis and analyzed some of their mechanisms of action. Methods In isolated segments of the guinea pig small intestine, peristalsis was triggered by a perfusion-induced rise of the intraluminal pressure. The peristaltic pressure threshold to elicit a peristaltic wave was used to quantify drug effects on peristalsis. Vehicle (Tyrode's solution), clonidine (10 nM-100 microm), or dexmedetomidine (0.1-100 nM) were added extraserosally to the organ bath. In other series of experiments, clonidine or dexmedetomidine was administered after pretreatment with yohimbine, prazosin, apamin, naloxone, or vehicle. Clonidine was also tested after blockade of NO synthase with L-NAME and in the presence of the inactive enantiomer D-NAME. Results Clonidine and dexmedetomidine concentration-dependently increased peristaltic pressure threshold and inhibited peristalsis (clonidine: EC50 = 19.6 microm; dexmedetomidine: EC50 = 12.0 nM). The inhibition caused by clonidine could be prevented by pretreatment with yohimbine, naloxone, and apamin, but not by prazosin, L-NAME, or D-NAME. Inhibition caused by dexmedetomidine was prevented by yohimbine only.(50) (50) Conclusions The results reveal that clonidine and, much more potently, dexmedetomidine inhibit peristalsis of the guinea pig ileum. The inhibition is caused by interaction with alpha2 adrenoceptors and, in the case of clonidine, also involves activation of small conductance Ca2+ -activated potassium channels and endogenous opioidergic pathways.

scholarly journals Why the Readiness Potential Does Not Disprove Free Will

2021 ◽  
Vol 14 ◽  
pp. 124-132
Author(s):  
Even Totland ◽  
Keyword(s):  
Free Will ◽  
Human Subject ◽  
Human Subjects ◽  
Readiness Potential ◽  
Neural Processes ◽  
Series Of Experiments ◽  
The Brain

Neuroscientist Benjamin Libet has conducted a series of experiments that reveal the existence of certain neural processes in the brain of human subjects, initiating an action prior to the human subject’s intention to act, thus seemingly threatening our idea of free will. The purpose of this paper is to show how these processes do not disprove any idea of free will one might have as one would, if accepting such a thesis, be committing two distinct mereological fallacies and ultimately, would treat the human subject as inhabiting some of its parts as opposed to being the sum of its parts.

scholarly journals The Standardisation of Tuberculin

1930 ◽  
Vol 30 (2) ◽  
pp. 221-238 ◽  
Author(s):  
Robert Philip ◽  
A. G. McKendrick ◽  
R. S. Begbie ◽  
W. O. Kermack ◽  
Donald Stewart
Keyword(s):  
Guinea Pig ◽  
Human Subject ◽  
Chemical Method ◽  
Human Subjects ◽  
Skin Surface ◽  
Accurate Estimation ◽  
Allergic Reactions ◽  
The One ◽  
Intracutaneous Injection

It will be convenient to summarise the results of the foregoing observations and the conclusions which appear justified by a comparison of results obtained by the two methods of standardisation under consideration, namely (1) cutaneous, on the human subject, and (2) intracutaneous (intradermal) on the guinea-pig.1. In the absence of knowledge regarding the essential chemistry of tuberculin, a chemical method of standardisation is excluded.2. The phenomena of allergic reactivity point to a biological basis for standardisation.3. The allergic reactions have been studied in the sensitised animal and in the human tuberculised subject.4. Tuberculins of unknown potency have been compared with a tuberculin of known potency which has been selected as standard.5. Comparative observations have been made as between the standard and the unknown tuberculin, by (a) making use of different dilutions, and (6) assessing results in animals (sensitised) and in human (tuberculised) subjects.6. In the sensitised guinea-pig the intracutaneous method has been used, and a definite procedure followed in the determination of results.7. In the human tuberculised subject the cutaneous method has been used, and similar procedure followed for the determination of results. The method and results are illustrated in the text.8. The results in the two sets of observations are definite and comparable. Both afford a basis for standardisation.9. The average probable errors by the two methods are approximately equal, that is, the accuracy of the one method is approximately equal to that of the other.10. If this be so, a strong plea may be advanced in favour of the human test. Shortly expressed: human (tuberculised) subjects are readily available for observation. The procedure involves little preparation, and the results are easily read with exactness. The tuberculin under test is to be used thereafter in relation to the human subject. This fact enhances the value of the test observations.If it be objected that in intracutaneous injection the amount of tuberculin introduced is measured more precisely, it may fairly be maintained that the droplet application of tuberculin is limited to a sharply defined area of skin surface and, further, that the clear skin of the human subject allows of more accurate estimation of the diameters of the areas of reaction.The present enquiry has shown that assays on a variety of human (tuberculised) subjects yield consistent results, and similarly, assays on various animals give consistent results. Yet the animal results are not always contistent with the human results. The explanation of the discrepancy is not very clear. It is not impossible that certain strains of tuberculin act in less degree on the human subject and in greater degree on the animal, and conversely. The occurrence of such differences might be misleading and even involve risk, if standardisation tests were limited to animals without control from observations on the human subject.If we grant, as the records have shown, that the procedure in relation to the human subject is sound and is innocuous to the human subject of the test, much may be said for the simplicity of the method and for the clarity of results obtained. As the tuberculins under test are destined for use on human subjects—for diagnostic and therapeutic purposes—it would seem reasonable, and probably safer, to base the standardisation of tuberculin (for human purposes) on observations in relation to man.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

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