Growth of yeast cultures as in vitro model for investigating homœopathic medicines

AbstractThe question of a sensitive response in the growth of yeast cell cultures to treatments with homœopathic dilutions (potencies), has been raised in a number of recent publications,1,2,3. A further set of growth trials with this model performed at our Institute are reported here.The actions of 8x to 16x decimal potencies of AgNO3, CuSO4, HgCl2, and NaCl on the growth of cell cultures of Schizosaccharomyces pombe were investigated. This included a set of trials with experimental poisoning of the cells by toxic levels of CuSO4 and subsequent study of the remedial action by potencies of the same substance.Yeast cells were grown in batch culture in a glucose/malt extract medium. Growth was interrupted shortly before the end of the exponential growth phase and total cell concentrations were assessed by means of a Coulter Counter. The obtained data were subjected to statistical analysis. Each treatment range was tested in three separate trials.The results obtained failed to indicate any response in the growth rate of Sch. pombe to the potency treatments.

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Comment on the paper, “Growth of yeast cultures as in vitro model for investigating homœopathic remedies”

British Homeopathic Journal ◽

10.1016/s0007-0785(85)80042-9 ◽

1985 ◽

Vol 74 (02) ◽

pp. 93-96 ◽

Cited By ~ 4

Author(s):

Rose D. Baker ◽

C.W. Smith

Keyword(s):

Saccharomyces Cerevisiae ◽

Growth Rate ◽

In Vitro Model ◽

Mean Value ◽

Statistical Test ◽

Yeast Cultures ◽

The Mean ◽

Vitro Model

SummaryA recent paper by Steffen1 repeating earlier work by Jones et al.2 failed to confirm any effect of potencies of Pulsatilla on the growth rate of cultures of Saccharomyces cerevisiae which had previously been reported.We find that using a more specific statistical test on the results given by Steffen1 it can be shown that these results contain almost the same periodicity with potency that Jones et al.2 reported, although only corresponding to a variation of ±1.5% about the mean value.

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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