scholarly journals A gene fusion event in the evolution of aminoacyl-tRNA synthetases

FEBS Letters ◽  
2000 ◽  
Vol 470 (3) ◽  
pp. 300-304 ◽  
Author(s):  
Eric Berthonneau ◽  
Marc Mirande
Keyword(s):  
Gene Fusion ◽  
Fusion Event ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases ◽  
Gene Fusion Event

scholarly journals Origin of the plant Tm-1-like gene via two independent horizontal transfer events and one gene fusion event

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Zefeng Yang ◽  
Li Liu ◽  
Huimin Fang ◽  
Pengcheng Li ◽  
Shuhui Xu ◽  
...  
Keyword(s):  
Horizontal Transfer ◽  
Gene Fusion ◽  
Fusion Event ◽  
Gene Fusion Event

scholarly journals A functionally conserved STORR gene fusion in Papaver species that diverged 16.8 million years ago

2021 ◽  
Author(s):  
Theresa Catania ◽  
Yi Li ◽  
Thilo Winzer ◽  
David Harvey ◽  
Fergus Meade ◽  
...  
Keyword(s):  
Gene Fusion ◽  
Gene Tree ◽  
Genomic Analysis ◽  
Duplication Event ◽  
Opium Poppy ◽  
Fusion Event ◽  
Genome Duplication Event ◽  
Benzylisoquinoline Alkaloid ◽  
Gene Fusion Event ◽  
Important Exception

The STORR gene fusion event is considered a key step in the evolution of benzylisoquinoline alkaloid (BIA) metabolism in opium poppy as the resulting bi-modular protein performs the isomerization of (S)- to (R)- reticuline which is required for morphinan biosynthesis. Our previous analysis of the opium poppy genome suggested the STORR gene fusion event occurred before a whole genome duplication event 7.2 million years ago. Here we use a combination of phylogenetic, transcriptomic, metabolomic, biochemical and genomic analysis to investigate the origin of the STORR gene fusion across the Papaveraceae family. The pro-morphinan/morphinan subclass of BIAs was present in a subset of 10 Papaver species including P. somniferum (opium poppy) and this correlated with the presence of the STORR gene fusion with one important exception. P. californicum does not produce morphinans but it does contain a STORR gene fusion that epimerizes (S)- to (R)- reticuline when heterologously expressed in yeast. The high similarity of the amino acid sequence linking the two modules of STORR along with phylogenetic gene tree analysis strongly suggests the gene fusion occurred only once and between 17-25 million years ago before the separation of P. californicum from the other Papaver species. We discovered that the most abundant BIA in P. californicum is (R)- glaucine, a member of the aporphine subclass of BIAs. Only the (S) isomer of this compound has previously been reported from nature. These results lead us to conclude that the function of the STORR gene fusion is not exclusive to morphinan production in the Papaveraceae.

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