M1828 Intercellular Space Distance Is Increased in Refractory Heartburn Patients with Positive Symptom Index Regardless of Whether Symptoms Are Caused By Acid or Nonacid Reflux: A Study Using Impedance-pH and Electron Microscopy

Abstract Background Food addiction (FA) is a controversial concept, denoting the craving for certain foods. Given the little information available, the aim of this study was to evaluate the possible relationships between FA and full-blown and subthreshold psychopathology or eating behaviors in subjects consulting nutritional biologists. Materials and methods Three-hundred and fifty subjects completed the following self-questionnaires: Yale Food Addiction Scale (YFAS), Structured Clinical Interview for Mood Spectrum, Self-Report, Lifetime Version (MOOD-SR-LT), Symptom Checklist-90-Revised (SCL-90-R), Structured Clinical Interview for Anorexic-Bulimic Spectrum, Self-Report, Lifetime Version (ABS-SR-LT). Results Most of the subjects were women (n = 278) and the remaining were 72 men. A large proportion of the subjects (77.1%) had a YFAS score <3 and 22.9% ≥3, with no difference between men and women. The YFAS scores ≥3 were significantly and positively related to the all ABS-SR-LT domains, as well as to three dimensions (Depression, Hypomania, Rhythmicity) of the MOOD-SR-LT, and some SCL-90-R domains (Sensitivity, Psychoticism, General Symptom Index and Positive Symptom). Conclusion Our data, while indicating that FA is related to different subthreshold psychopathological domains, in particular, with both depressive and manic symptoms, as well as with rhythmicity of mood spectrum, or with eating subthreshold symptoms, would suggest that it might be a dimension underlying different conditions or symptom clusters.

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Bisphosphonate’s Effect on Tongue Mucosa: An Experimental Electron Microscopy Study

Medicina ◽

10.3390/medicina56020051 ◽

2020 ◽

Vol 56 (2) ◽

pp. 51

Author(s):

Theodora Papamitsou ◽

Antonios Morsi-Yeroyannis ◽

Anastasios Papanastasiou ◽

Nikolaos Bakalopoulos ◽

Eva-Maria Dietrich ◽

...

Keyword(s):

Electron Microscopy ◽

Intercellular Space ◽

Wistar Rats ◽

Middle Layer ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Control Group ◽

The Past ◽

Transmission Electron ◽

Experimental Group

Background and objectives: Bisphosphonates (BPs) are selective inhibitors of osteoclasts, used for the treatment of bone disorders. The objective of this study is to investigate the possible effects of BPs on the tongue’s mucosa. Materials and Methods: Specimens of the tongue of 20 female 12-month old Wistar rats were taken. Ten were used as control group, while in the remaining alendronate (Fosamax, Merck) was administered per os from 13 weeks. Observation of the harvested samples was made by Transmission Electron Microscopy (TEM). Results: In the experimental group, focal alterations were observed to various extent in all specimens. The basement membrane was intact. Furthermore, an increase at the intercellular space was observed, predominantly at the middle layer, and the desmosomes were disorganized. In the lamina propria focal edema was observed. Conclusions: Investigation on the effect of BPs on the tongue’s mucosa through TEM hasn’t been documented in the past. According to our results, BPs seem to cause mild mucosal lesions on the tongue.

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Observations on the nephridial system of the cestode Moniezia expansa (Rud., 1805)

Parasitology ◽

10.1017/s0031182000082408 ◽

1969 ◽

Vol 59 (2) ◽

pp. 449-459 ◽

Cited By ~ 30

Author(s):

R. E. Howells

Keyword(s):

Electron Microscopy ◽

Fine Structure ◽

Epithelial Cells ◽

Connective Tissue ◽

Intercellular Space ◽

Technical Assistance ◽

Light And Electron Microscopy ◽

Flame Cell ◽

Research Scholarship ◽

Research Facilities

The nephridial system of M. expansa has been studied using light and electron microscopy, and a number of histochemical techniques have been used on sections of the worm. The organization of the nephridial system and the fine structure of the flame cells and the nephridial ducts are described. Pores, which connect the nephridial lumen to the intercellular space of the connective tissue, exist at the junction of a flame cell and a nephridial duct. These pores may be considered nephrostomes and the system therefore is not protonephridial as defined by Hyman (1951).The epithelium lining the nephridial ducts has a structure which suggests that it is metabolically active. It is postulated that the beating of the cilia of the flame cells draws fluid into the ducts via the nephrostomes, with absorption and/or secretion of solutes being carried out by the epithelial cells of the duct walls. The function of the nephridial system is discussed.I am grateful to Professor James Brough for the provision of research facilities at the Department of Zoology, University College, Cardiff, andtoDrD. A. Erasmus for much helpful advice during the course of the work. I wish to thank Professors W. Peters and T. Wilson for critically reading the manuscript and Miss M. Williams and Mr T. Davies for expert technical assistance.I also wish to thank the Veterinary Inspector and his staff at the Roath Abattoir, Cardiff, for their kind co-operation and assistance in obtaining material.The work was carried out under the tenure of an S.R.C. research scholarship.

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Borrowing Benefits: Group Treatment With Clinical Emotional Freedom Techniques Is Associated With Simultaneous Reductions in Posttraumatic Stress Disorder, Anxiety, and Depression Symptoms

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2156587218756510 ◽

2018 ◽

Vol 23 ◽

pp. 215658721875651 ◽

Cited By ~ 2

Author(s):

Dawson Church ◽

Dennis House

Keyword(s):

Posttraumatic Stress Disorder ◽

Posttraumatic Stress ◽

Group Treatment ◽

Stress Disorder ◽

Positive Symptom ◽

Depression Symptoms ◽

Depression And Anxiety ◽

Symptom Index ◽

Emotional Freedom Techniques ◽

The Relationship

Clinical Emotional Freedom Techniques (EFT) is an evidence-based treatment for depression and anxiety. The current study sought to elucidate the relationship between posttraumatic stress disorder (PTSD), depression, and anxiety in a nonclinical population. The sample (N = 81) comprised participants at five 2-day EFT workshops. All groups used an EFT protocol called Borrowing Benefits, in which the group facilitator works with a single client while other participants self-apply EFT. Participants were assessed on 9 specific conditions as well as on the breadth (Positive Symptom Total [PST]) and depth (General Symptom Index [GSI]) of psychological distress. Physical pain and addictive cravings were also assessed. Significant reductions were observed in all measures (P < .03). Associations between PST, GSI, and PTSD were significant (P < .026). Participants maintained all gains at 6-month follow-up (P < .02) with the exception of the Hostility subscale, while Cohen’s d = 0.54 indicated a moderate treatment effect for PTSD. The relationship between psychological and physiological conditions identified in this study is consistent with that found in other studies. Group treatment is cost-effective and efficient, and the efficacy of EFT in groups indicates the utility of the Borrowing Benefits technique.

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Comparison of Light and Electron Microscopy in Measurement of Esophageal Intercellular Space in Children

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/mpg.0000000000000373 ◽

2014 ◽

Vol 59 (2) ◽

pp. 232-236 ◽

Cited By ~ 1

Author(s):

Muhammad A. Altaf ◽

Thomas Ciecierega ◽

Sara Szabo ◽

Adrian Miranda ◽

Christina Gorges ◽

...

Keyword(s):

Electron Microscopy ◽

Intercellular Space ◽

Light And Electron Microscopy

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What constitutes a 'positive symptom index' for the diagnosis of gastro-oesophageal reflux- related pain?

http://isrctn.org/> ◽

10.1186/isrctn74638573 ◽

2012 ◽

Author(s):

J De Caestecker

Keyword(s):

Oesophageal Reflux ◽

Positive Symptom ◽

Symptom Index

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S1899 Physiologic Acid Reflux and Positive Symptom-Index, Is It Physiologic?

Gastroenterology ◽

10.1016/s0016-5085(09)61314-7 ◽

2009 ◽

Vol 136 (5) ◽

pp. A-288

Author(s):

Jose J. Herrera Esquivel ◽

Juan Octavio Alonso-Larraga ◽

García R. Luis Eduardo ◽

Ignacio Guerrero-Hernández ◽

Martha Fernandez Rosales

Keyword(s):

Acid Reflux ◽

Positive Symptom ◽

Symptom Index

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Characteristics of Symptomatic Reflux Episodes in Patients with Non-Erosive Reflux Disease Who Have a Positive Symptom Index on Proton Pump Inhibitor Therapy

Digestion ◽

10.1159/000309483 ◽

2010 ◽

Vol 82 (3) ◽

pp. 156-161 ◽

Cited By ~ 13

Author(s):

Katsuhiko Iwakiri ◽

Hirohito Sano ◽

Yuriko Tanaka ◽

Noriyuki Kawami ◽

Mariko Umezawa ◽

...

Keyword(s):

Proton Pump Inhibitor ◽

Proton Pump ◽

Reflux Disease ◽

Proton Pump Inhibitor Therapy ◽

Inhibitor Therapy ◽

Positive Symptom ◽

Symptom Index ◽

Symptomatic Reflux

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SURFACE SPECIALIZATIONS OF FUNDULUS CELLS AND THEIR RELATION TO CELL MOVEMENTS DURING GASTRULATION

The Journal of Cell Biology ◽

10.1083/jcb.32.1.139 ◽

1967 ◽

Vol 32 (1) ◽

pp. 139-153 ◽

Cited By ~ 71

Author(s):

J. P. Trinkaus ◽

Thomas L. Lentz

Keyword(s):

Electron Microscopy ◽

Intercellular Space ◽

Plasma Membranes ◽

Time Lapse ◽

Cell Movements ◽

Membrane Activity ◽

Inner Surface ◽

Apical Junctions ◽

Controlled Flow ◽

Marginal Cells

Cell movements in Fundulus blastoderms during gastrulation were studied utilizing time-lapse cinemicrography and electron microscopy. Time-lapse films reveal that cells of the enveloping layer undulate and sometimes separate briefly but remain together in a cohesive layer. During epiboly, the marginal enveloping layer cells move over the periblast as it expands over the yolk sphere. Movement occurs as a result of ruffled membrane activity of the free borders of the marginal cells. Deep blastomeres become increasingly active during blastula and gastrula stages. Lobopodia project from the blastomeres in blastulae and adhere to other cells in gastrulae, giving the cells traction for movement. Contact specializations are formed by the lateral adjacent plasma membranes of enveloping layer cells. An apical junction is characterized by an intercellular gap of 60–75 A. Below this contact, the plasma membranes are separated by 120 A or more. In mid-gastrulae, cytoplasmic fibrils occur adjacent to some apical junctions, and small desmosomes appear below the apical junction. Septate desmosomes also appear at this time. A junction with an intercellular gap of 60 A occurs between marginal enveloping layer cells and periblast. Contacts between deep blastomeres become numerous in gastrulae and consist of contacts at the crests of surface undulations, short areas of contact in which the plasma membranes are 60 or 120 A apart, and long regions characterized by a 200-A intercellular gap. Lobopodia contact other blastomeres only in gastrulae. These junctions contain a 200-A intercellular space. Some deep blastomeres are in contact with the tips of periblast microvilli. The mechanism of epiboly in Fundulus is discussed and reevaluated in terms of these observations. The enveloping layer is adherent to the margin of the periblast and moves over it as a coherent cellular sheet. Periblast epiboly involves a controlled flow of cytoplasm from the thicker periblast into the thinner yolk cytoplasmic layer with which it is continuous. Deep cells move by adhering to each other, to the inner surface of the enveloping layer, and to the periblast.

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