Su1201 The Expression of the 67 kDa Laminin Receptor (LR) or Green Tea Catechin Epigallocatechin-3-Gallate (EGCG) Receptor May Explain the Different Responses to EGCG in the Cell Lines Infected With H. pylori

2014 ◽  
Vol 146 (5) ◽  
pp. S-400
Author(s):  
Prakitpunthu Tomtitchong ◽  
Jean E. Crabtree
Keyword(s):  
Cell Lines ◽  
Green Tea ◽  
Laminin Receptor ◽  
Green Tea Catechin ◽  
H Pylori

M1175 The Green Tea Catechin Epigallocatechin-3-Gallate Inhibits H. pylori-Induced and EGF-Induced ERK Signaling in A431 Epithelial Cells

2010 ◽  
Vol 138 (5) ◽  
pp. S-348
Author(s):  
Prakitpunthu Tomtitchong ◽  
Yiqi Du ◽  
Philip A. Robinson ◽  
Jean E. Crabtree
Keyword(s):  
Epithelial Cells ◽  
Green Tea ◽  
Erk Signaling ◽  
Green Tea Catechin ◽  
H Pylori

scholarly journals Specific killing of multiple myeloma cells by (-)-epigallocatechin-3-gallate extracted from green tea: biologic activity and therapeutic implications

Blood ◽  
2006 ◽  
Vol 108 (8) ◽  
pp. 2804-2810 ◽  
Author(s):  
Masood A. Shammas ◽  
Paola Neri ◽  
Hemanta Koley ◽  
Ramesh B. Batchu ◽  
Robert C. Bertheau ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Cell Lines ◽  
Green Tea ◽  
Fas Ligand ◽  
Mononuclear Cells ◽  
Apoptotic Cell ◽  
Growth Arrest ◽  
Laminin Receptor ◽  
Peripheral Blood Mononuclear ◽  
Myeloma Cells

AbstractEpigallocatechin-3-gallate (EGCG), a polyphenol extracted from green tea, is an antioxidant with chemopreventive and chemotherapeutic actions. Based on its ability to modulate growth factor-mediated cell proliferation, we evaluated its efficacy in multiple myeloma (MM). EGCG induced both dose- and time-dependent growth arrest and subsequent apoptotic cell death in MM cell lines including IL-6-dependent cells and primary patient cells, without significant effect on the growth of peripheral blood mononuclear cells (PBMCs) and normal fibroblasts. Treatment with EGCG also led to significant apoptosis in human myeloma cells grown as tumors in SCID mice. EGCG interacts with the 67-kDa laminin receptor 1 (LR1), which is significantly elevated in myeloma cell lines and patient samples relative to normal PBMCs. RNAi-mediated inhibition of LR1 resulted in abrogation of EGCG-induced apoptosis in myeloma cells, indicating that LR1 plays an important role in mediating EGCG activity in MM while sparing PBMCs. Evaluation of changes in gene expression profile indicates that EGCG treatment activates distinct pathways of growth arrest and apoptosis in MM cells by inducing the expression of death-associated protein kinase 2, the initiators and mediators of death receptor-dependent apoptosis (Fas ligand, Fas, and caspase 4), p53-like proteins (p73, p63), positive regulators of apoptosis and NF-κB activation (CARD10, CARD14), and cyclin-dependent kinase inhibitors (p16 and p18). Expression of related genes at the protein level were also confirmed by Western blot analysis. These data demonstrate potent and specific antimyeloma activity of EGCG and provide the rationale for its clinical evaluation.

Specific Killing of Multiple Myeloma Cancer Cells by Epigallocatechin-3-Gallate Extracted from Green Tea.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2461-2461 ◽  
Author(s):  
Masood A. Shammas ◽  
Hemanta Koley ◽  
Ramesh B. Batchu ◽  
Paola Neri ◽  
Pierfrancesco Tassone ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Green Tea ◽  
Plasma Cells ◽  
Apoptotic Cell ◽  
Receptor Gene ◽  
Apoptotic Cell Death ◽  
Laminin Receptor ◽  
Caspase 8 ◽  
Egcg Treatment

Abstract Epidemiological studies have demonstrated chemopreventive effects of green tea in several types of cancers. The active ingredient present in green tea, and not black tea, is epigallocatechin-3-gallate (EGCG). EGCG has been shown to interfere with multi-step carcinogenesis and it exerts its effects in pharmacologically attainable non-toxic concentrations. We examined the effects of EGCG on growth of MM cells. We observed dose- and time-dependent induction of apoptosis in MM cells following EGCG treatment. Apoptotic cell death, as measured by annexin V labeling was 38 ± 6% and 60 ± 8% at 24h with 100 mM and 200 mM EGCG concentration, respectively. This increased to 73 ± 5% and 88 ± 2% at 72h, respectively. Importantly, no significant apoptosis was observed in normal cells exposed to 200 μM EGCG. The apoptotic cell death was predominantly caspase 9 dependent and not caspase 8 dependent as evaluated using specific caspase 8 and 9 inhibitors. The anti-tumor effects of EGCG has been demonstrated to be mediated via a metastasis-associated 67-kDa laminin receptor. Gene expression profile of MM cell lines (n=6) and patient myeloma cells (n=15) compared to normal plasma cells (n=3) showed 3.1 ± 1 and 3.9 ± 0.7-fold elevation of laminin receptor respectively. Significant upregulation of this receptor was observed in 6 of 6 MM cell lines and 14 of 15 MM patient samples. We are currently investigating possible role of laminin receptor in EGCG mediated cell death in MM and combinations that may enhance EGCG-mediated MM cell death. These data suggest EGCG as a promising natural agent with excellent therapeutic index.

Versatile Health Benefits of Catechin from Green Tea (Camellia sinensis)

2019 ◽  
Vol 15 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Satheesh Babu Natarajan ◽  
Suriyakala Perumal Chandran ◽  
Sahar Husain Khan ◽  
Packiyaraj Natarajan ◽  
Karthiyaraj Rengarajan
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Health Benefits ◽  
Epigallocatechin Gallate ◽  
Extraction Methods ◽  
Major Constituent ◽  
Epicatechin Gallate ◽  
Wide Range ◽  
Green Tea Catechin ◽  
Anti Inflammatory

Background: Tea (Camellia sinensis, Theaceae) is the second most consumed beverage in the world. Green tea is the least processed and thus contain rich antioxidant level, and believed to have most of the health benefits. </p><p> Methods: We commenced to search bibliographic collection of peer reviewed research articles and review articles to meet the objective of this study. </p><p> Results: From this study, we found that the tea beverage contains catechins are believed to have a wide range of health benefits which includes neuroprotective, anti-inflammatory, antiulcer, antiviral, antibacterial, and anti-parasitic effects. The four major catechin compounds of green tea are epigallocatechin (EGC), epicatechin (EC), epigallocatechin gallate (EGCG), and epicatechin gallate (ECG), of which EGCG is the major constituent and representing 50-80% of the total catechin content. And also contain xanthine derivatives such as caffeine, theophylline, and theobromine, and the glutamide derivative theanine. It also contains many nutritional components, such as vitamin E, vitamin C, fluoride, and potassium. We sum up the various green tea phytoconstituents, extraction methods, and its medicinal applications. </p><p> Conclusion: In this review article, we have summarized the pharmacological importance of green tea catechin which includes antioxidant potential, anti-inflammatory, antimicrobial, anticancer, antidiabetic and cosmetic application.

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