Tu1281 - The Protective Role of Nadph Oxidase 4 in Dextran Sulfate Sodium-Induced Colitis Mouse Model

The role of the gut microbiota in the pathogenesis of inflammatory bowel disease (IBD) has been in focus for decades. Although metagenomic observations in patients/animal colitis models have been attempted, the microbiome results were still indefinite and broad taxonomic presumptions were made due to the cross-sectional studies. Herein, we conducted a longitudinal microbiome analysis in a dextran sulfate sodium (DSS)-induced colitis mouse model with a two-factor design based on serial DSS dose (0, 1, 2, and 3%) and duration for 12 days, and four mice from each group were sacrificed at two-day intervals. During the colitis development, a transition of the cecal microbial diversity from the normal state to dysbiosis and dynamic changes of the populations were observed. We identified genera that significantly induced or depleted depending on DSS exposure, and confirmed the correlations of the individual taxa to the colitis severity indicated by inflammatory biomarkers (intestinal bleeding and neutrophil-derived indicators). Of note, each taxonomic population showed its own susceptibility to the changing colitis status. Our findings suggest that an understanding of the individual susceptibility to colitis conditions may contribute to identifying the role of the gut microbes in the pathogenesis of IBD.

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The Role of Zinc and Metallothionein in the Dextran Sulfate Sodium-Induced Colitis Mouse Model

Digestive Diseases and Sciences ◽

10.1007/s10620-007-9765-9 ◽

2007 ◽

Vol 52 (9) ◽

pp. 2113-2121 ◽

Cited By ~ 43

Author(s):

C. D. Tran ◽

J. M. Ball ◽

S. Sundar ◽

P. Coyle ◽

G. S. Howarth

Keyword(s):

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium

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The role of mutant Apc in the development of dysplasia and cancer in the mouse model of dextran sulfate sodium–induced colitis

Gastroenterology ◽

10.1053/gast.2001.29609 ◽

2001 ◽

Vol 121 (6) ◽

pp. 1407-1416 ◽

Cited By ~ 66

Author(s):

Harry S. Cooper ◽

Lynette Everley ◽

Wen–Chi Chang ◽

Gordon Pfeiffer ◽

Bryan Lee ◽

...

Keyword(s):

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium

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Fusobacterium nucleatum impedes remission of colitis in a mouse model

Bioscience Biotechnology and Biochemistry ◽

10.1093/bbb/zbab029 ◽

2021 ◽

Vol 85 (5) ◽

pp. 1235-1242

Author(s):

Taiga Yamashita ◽

Shoya Tai ◽

Takamitsu Tsukahara ◽

Ryo Inoue

Keyword(s):

Drinking Water ◽

Mouse Model ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Fusobacterium Nucleatum ◽

Recovery Phase ◽

Phosphate Buffered Saline ◽

Cecal Mucosa ◽

Activity Indices

ABSTRACT The role of Fusobacterium nucleatum, often associated with intestinal diseases, in the remission of dextran sulfate sodium (DSS)-induced colitis was investigated. Female mice were divided into groups DC (DSS control) and DF (DSS + F. nucleatum). F. nucleatum (1.0 × 1010 cfu/mouse/day) in phosphate-buffered saline (PBS) was orally given to DF, while DC had PBS only. All mice had DSS in drinking water. In Experiment 1, mice underwent 2 inflammation phases, an in-between recovery phase and had their disease activity indices (DAI) calculated. Experiment 2 was similarly conducted, except that mice were dissected 3 days postrecovery, and had blood and colonic mucosal samples collected. In Experiment 1, DF had significantly (P < .05) higher DAI than DC, during the recovery and 2nd inflammation phases. In Experiment 2, genus Bacteroides was significantly (P < .05) higher and family Lachnospiraceae significantly lower in cecal mucosa-associated microbiota of DF than in that of DC. We concluded that F. nucleatum can impede colitis remission.

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