Expression of GTPase-deficient G alpha 16 inhibits Swiss 3T3 cell growth

Swiss/3T3 cell cultures were harvested with 0.05% collagenase and after centrifugation the pellet was prepared by the freeze-fracture/freeze-drying (FFFD) method for bulk-specimen X-ray microanalysis. Time-dependent variations in the intracellular monovalent elemental concentrations (Na+, K+ and Cl-) as well as of the Na+/K+ ratio were followed for 120 min subsequent to harvesting. The quantitative measurements revealed a very considerable increase in the intracellular Na+ and Cl- accompanied by a decrease in the K+ concentration as soon as 5 min after harvesting. The Na+/K+ ratio had increased by this time to about 1.5 on average. These changes indicate a sustained depolarization of the cell membrane. During the first 60 min this depolarization tended to normalize as demonstrated by an exponential decrease in the intracellular Na+ and Cl- and an increase in the K+ content involving a decrease in the Na+/K+ ratio. The total intracellular monovalent ion concentration remained almost constant during this post-harvesting period. These results suggest that harvesting represents a serious depolarizing stimulus to the cells, the consequences of which are restored only after 1–2h. These alterations should be taken into consideration during various experimental designs when using anchorage-dependent cell cultures.

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Effects of pertussis toxin on growth factor-stimulated inositol phosphate formation and DNA synthesis in Swiss 3T3 cells

Biochemical Journal ◽

10.1042/bj2490917 ◽

1988 ◽

Vol 249 (3) ◽

pp. 917-920 ◽

Cited By ~ 38

Author(s):

C W Taylor ◽

D M Blakeley ◽

A N Corps ◽

M J Berridge ◽

K D Brown

Keyword(s):

Growth Factor ◽

Dna Synthesis ◽

Pertussis Toxin ◽

Inositol Phosphates ◽

Inositol Phosphate ◽

Phosphoinositide Hydrolysis ◽

Polypeptide Growth Factors ◽

Swiss 3T3 ◽

Swiss 3T3 Cell ◽

Stimulation Of

We have compared the effects of pretreatment of Swiss 3T3 cell with pertussis toxin on the stimulation of DNA synthesis and phosphoinositide hydrolysis in response to a wide variety of mitogens. The toxin substantially inhibited the stimulation of DNA synthesis in response to a phorbol ester or various peptide and polypeptide growth factors irrespective of their ability to activate phosphoinositidase C. Production of inositol phosphates in response to platelet-derived growth factor, fibroblast growth factor and prostaglandin F2 alpha were unaffected by the toxin while bombesin- and vasopressin-stimulated formation of inositol phosphates were inhibited by only 27 and 23% respectively. These results argue against a major role for a pertussis toxin-sensitive G protein in coupling any of these mitogen receptors to activation of a phosphoinositidase C. Furthermore, the results suggest that the widespread inhibitory effects of pertussis toxin on mitogen-stimulated DNA synthesis may be unrelated to the toxin's limited actions on phosphoinositide hydrolysis.

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Sulfated mucopolysaccharides from normal Swiss 3T3 cell line and its tumorigenic mutant ST1: Possible role of chondroitin sulfates in neoplastic transformation

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(78)90774-x ◽

1978 ◽

Vol 84 (3) ◽

pp. 794-801 ◽

Cited By ~ 25

Author(s):

Carl P. Dietrich ◽

Hugo A. Armelin

Keyword(s):

Cell Line ◽

Neoplastic Transformation ◽

Chondroitin Sulfates ◽

Swiss 3T3 ◽

Swiss 3T3 Cell

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Differential effects of n-6 and n-3 polyunsaturated fatty acids on cell growth and early gene expression in Swiss 3T3 fibroblasts

Journal of Cellular Physiology ◽

10.1002/(sici)1097-4652(199609)168:3<618::aid-jcp14>3.0.co;2-v ◽

1996 ◽

Vol 168 (3) ◽

pp. 618-624 ◽

Cited By ~ 29

Author(s):

Ulrich Danesch ◽

Peter C. Weber ◽

Alois Sellmayer

Keyword(s):

Gene Expression ◽

Fatty Acids ◽

Cell Growth ◽

Polyunsaturated Fatty Acids ◽

Early Gene ◽

Early Gene Expression ◽

Differential Effects ◽

3T3 Fibroblasts ◽

Swiss 3T3 ◽

Swiss 3T3 Fibroblasts

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Production of plasminogen activator by established cell lines of mouse origin.

The Journal of Cell Biology ◽

10.1083/jcb.73.1.47 ◽

1977 ◽

Vol 73 (1) ◽

pp. 47-55 ◽

Cited By ~ 42

Author(s):

D B Rifkin ◽

R Pollack

Keyword(s):

Plasminogen Activator ◽

Cell Lines ◽

3T3 Cells ◽

Established Cell Lines ◽

Embryo Cultures ◽

Established Cell ◽

Swiss 3T3 ◽

Swiss 3T3 Cell ◽

Transformed Cell Lines ◽

3T3 Cell Lines

The correlation between malignant transformation and increased plasminogen activator synthesis has been studied in a variety of established cell lines. In contrast to the behavior of secondary mouse embryo cultures, which always show increased fibrinolytic activity when transformed, no such correlation was found within the BALB/c 3T3 line and its transformed derivatives. Cell lines were established from tumors initiated in BALB/c mice by several transformed cell lines. These lines were generally found to contain no more plasminogen activator than the cells used for inoculation. A correlation was found between transformation and plasminogen activator synthesis within Swiss 3T3 cell lines. However, the correlation was not maintained by serum revertants of transformed Swiss 3T3 cells.

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