scholarly journals Protein-specific energy requirements for protein transport across or into thylakoid membranes. Two lumenal proteins are transported in the absence of ATP.

1992 ◽  
Vol 267 (4) ◽  
pp. 2688-2696
Author(s):  
K Cline ◽  
W F Ettinger ◽  
S M Theg
2013 ◽  
Vol 44 (2s) ◽  
Author(s):  
Alessio Facello ◽  
Eugenio Cavallo ◽  
Raffaele Spinelli

Air pollution and fossil fuel reserves exhaustion are increasing the importance of the biomass-derived products, in particular wood, as source of clean and renewable energy for the production of electricity or steam. In order to improve the global efficiency and the entire production chain, we have to evaluate the energetic aspects linked to the process of transformation, handling and transport of these materials. This paper reports results on a comparison between two chippers of similar size using different cutting technology: disc and drum tool respectively. During trials, fuel consumption, PTO torque and speed, processing time and weight of processed material were recorded. Power demand, fuel consumption, specific energy and productivity were computed. The machine was fed with four different feedstock types (chestnut logs, poplar logs, poplar branches, poplar sawmill residues). 15 repetitions for each combination of feedstock-tool were carried out. The results of this study show that the disc tool requires, depending on the processed material, from 12 to 18% less fuel per unit of material processed than the drum tool, and consequently, from 12 to 16% less specific energy. In particular, the highest difference between tools was found in branches processing whereas the smallest was in poplar logs. Furthermore the results of the investigation indicate, that, in testing conditions, the productivity of drum tool is higher (8%) than disc tool.


Energy ◽  
2011 ◽  
Vol 36 (11) ◽  
pp. 6433-6441 ◽  
Author(s):  
Ali Motevali ◽  
Saeid Minaei ◽  
Mohammad Hadi Khoshtaghaza ◽  
Hamed Amirnejat

2012 ◽  
Vol 197 (4) ◽  
pp. 523-534 ◽  
Author(s):  
Jose M. Celedon ◽  
Kenneth Cline

Twin arginine translocation (Tat) systems transport large folded proteins across sealed membranes. Tat systems accomplish this feat with three membrane components organized in two complexes. In thylakoid membranes, cpTatC and Hcf106 comprise a large receptor complex containing an estimated eight cpTatC-Hcf106 pairs. Protein transport occurs when Tha4 joins the receptor complex as an oligomer of uncertain size that is thought to form the protein-conducting structure. Here, binding analyses with intact membranes or purified complexes indicate that each receptor complex could bind eight precursor proteins. Kinetic analysis of translocation showed that each precursor-bound site was independently functional for transport, and, with sufficient Tha4, all sites were concurrently active for transport. Tha4 titration determined that ∼26 Tha4 protomers were required for transport of each OE17 (oxygen-evolving complex subunit of 17 kD) precursor protein. Our results suggest that, when fully saturated with precursor proteins and Tha4, the Tat translocase is an ∼2.2-megadalton complex that can individually transport eight precursor proteins or cooperatively transport multimeric precursors.


CIRP Annals ◽  
2016 ◽  
Vol 65 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Paolo C. Priarone ◽  
Matteo Robiglio ◽  
Luca Settineri ◽  
Vincenzo Tebaldo

1999 ◽  
Vol 146 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Hiroki Mori ◽  
Elizabeth J. Summer ◽  
Xianyue Ma ◽  
Kenneth Cline

Prokaryotes and prokaryote-derived thylakoid membranes of chloroplasts share multiple, evolutionarily conserved pathways for protein export. These include the Sec, signal recognition particle (SRP), and Delta pH/Tat systems. Little is known regarding the thylakoid membrane components involved in these pathways. We isolated a cDNA clone to a novel component of the Delta pH pathway, Tha4, and prepared antibodies against pea Tha4, against maize Hcf106, a protein implicated in Delta pH pathway transport by genetic studies, and against cpSecY, the thylakoid homologue of the bacterial SecY translocon protein. These components were localized to the nonappressed thylakoid membranes. Tha4 and Hcf106 were present in ∼10-fold excess over active translocation sites. Antibodies to either Tha4 or Hcf106 inhibited translocation of four known Delta pH pathway substrate proteins, but not of Sec pathway or SRP pathway substrates. This suggests that Tha4 and Hcf106 operate either in series or as subunits of a heteromultimeric complex. cpSecY antibodies inhibited translocation of Sec pathway substrates but not of Delta pH or SRP pathway substrates. These studies provide the first biochemical evidence that Tha4 and Hcf106 are specific components of the Delta pH pathway and provide one line of evidence that cpSecY is used specifically by the Sec pathway.


Author(s):  
Lihua Ye ◽  
Hany S. EL-Mesery ◽  
Muhammad Muzamal Ashfaq ◽  
Yefan Shi ◽  
Hu Zicheng ◽  
...  

2017 ◽  
Vol 20 (3&4) ◽  
pp. 349-360
Author(s):  
A. F. Kheiralla ◽  
A. Yahya ◽  
M. Zohadie ◽  
W. Ishak

Power and energy requirements were measured with an instrumented tractor for rotary tilling in Serdang sandy clay loam soil.  The effects of travel speed and rotor speed upon the measured data were investigated.  Power model from orthogonal regression analysis was formulated based on linear and quadratic functions of travel speed and bite length.  Fuel consumption model from regression analysis was formulated based on linear tractor PTO power as well as linear equivalent tractor PTO power.  Fuel consumption rates predicted by ASAE D497.3 were found to be 25% to 28% overestimates of the values predicted by the model developed.  However, fuel consumption rates reported by OECD Tractor Test were found to be 1% to 9% lower than the fuel consumption rates predicted by the model developed.  A comparison of power and energy requirements for both powered and draught implements showed that the disk harrow was the most energy efficient implement in terms of fuel consumption and specific energy followed by the rotary tiller, disk plough and mouldboard.  Finally, average PTO power, fuel consumption, wheel slip, wheel power and specific energy for a powered implement are presented.


Energies ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 2389 ◽  
Author(s):  
Niccolò Pampuro ◽  
Patrizia Busato ◽  
Eugenio Cavallo

Hop cones, due to their essential flavor, are one of the four main ingredients for beer production. The paper reports the results on an investigation of the densification process of hop cones. This experiment investigated (i) the effects of compression pressure in the range of 40 to 80 MPa and pressure application time in the range of 10 to 40 s on the final density and durability of the compacts made from hop cones and ii) the specific compression energy required for the process. The specific compression energy requirements to compact hop cones ranged from 14.20 to 24.48 kJ kg−1. The final compact density values ranged from 515.2 to 876.6 kg m−3, while the durability percentage calculated ranged from 71% to 91%. The obtained results highlighted that compression pressure—in the range of 40–80 MPa—significantly affects the specific compression energy requirements, the final density and the durability of the produced compacts. In this experiment, pressure application time plays a key role in determining compacts density, while did not affect durability and compression energy requirements. Considering the specific compression energy values calculated in this experiment, it can be stated that the pressure agglomeration method described to compact hop cones is more efficient than pelletizing process which is typically characterized by specific energy values ranging from 19 to 90 kJ kg−1.


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