scholarly journals Differential regulation of human indoleamine 2,3-dioxygenase gene expression by interferons-gamma and -alpha. Analysis of the regulatory region of the gene and identification of an interferon-gamma-inducible DNA-binding factor.

1993 ◽  
Vol 268 (7) ◽  
pp. 5077-5084
Author(s):  
H.H. Hassanain ◽  
S.Y. Chon ◽  
S.L. Gupta
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Interferon Gamma ◽  
Regulatory Region ◽  
Differential Regulation ◽  
Indoleamine 2,3 Dioxygenase ◽  
Binding Factor ◽  
Dioxygenase Gene

Differential regulation of indoleamine 2,3-dioxygenase by lipopolysaccharide and interferon gamma in murine bone marrow derived dendritic cells

FEBS Letters ◽  
2007 ◽  
Vol 581 (7) ◽  
pp. 1449-1456 ◽  
Author(s):  
In Duk Jung ◽  
Chang-Min Lee ◽  
Young-Il Jeong ◽  
Jun Sik Lee ◽  
Won Sun Park ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Interferon Gamma ◽  
Differential Regulation ◽  
Murine Bone Marrow ◽  
Indoleamine 2,3 Dioxygenase

scholarly journals Altered Promoter and G-Box Binding Factor for 1-Deoxy-d-Xylulose-5-Phosphate Synthase Gene Grown from Poa pratensis Seeds after Spaceflight

2019 ◽  
Vol 20 (6) ◽  
pp. 1398
Author(s):  
Lu Gan ◽  
Yuehui Chao ◽  
Haotian Su ◽  
Yujing Ren ◽  
Shuxia Yin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Promoter Region ◽  
Poa Pratensis ◽  
Regulatory Region ◽  
Space Environment ◽  
Luciferase Assay ◽  
Dwarf Mutant ◽  
Transcript Accumulation ◽  
Binding Factor ◽  
Phosphate Synthase

In plant cells, the nucleus DNA is considered the primary site of injury by the space environment, which could generate genetic alteration. As the part of genomic mutation, genetic variation in the promoter region could regulate gene expression. In the study, it is observed that there is a deletion in the upstream regulatory region of the 1-deoxy-d-xylulose-5-phosphate synthase 1 gene (PpDXS1) of Poa pratensis dwarf mutant and the PpDXS1 transcript abundance is lower in the dwarf mutant. It is indicated that the deletion in the promoter region between wild type and dwarf mutant could be responsible for the regulation of PpDXS1 gene expression. The PpDXS1 promoter of dwarf mutant shows a lower activity as determined by dual luciferase assay in Poa pratensis protoplast, as well as the GUS activity is lower in transgenic Poa pratensis plant. To further investigate the effect of the deletion in the promoter region on PpDXS1 transcript accumulation, the transient assay and yeast one-hybrid experiment demonstrate that the deletion comprises a motif which is a target of G-box binding factor (GBF1), and the motif correlates with an increase in transactivation by GBF1 protein. Taken together, these results indicate that the deletion in the promoter of PpDXS1 isolated from dwarf mutant is sufficient to account for the decrease in PpDXS1 transcript level and GBF1 can regulate the PpDXS1 gene expression, and subsequently affect accumulation of various isoprenoids throughout the plant.

scholarly journals The DNA-binding factor Ctcf critically controls gene expression in macrophages

2013 ◽  
Vol 11 (1) ◽  
pp. 58-70 ◽  
Author(s):  
Tatjana Nikolic ◽  
Dowty Movita ◽  
Margaretha EH Lambers ◽  
Claudia Ribeiro de Almeida ◽  
Paula Biesta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Binding Factor

scholarly journals Rapid activation of proteins that interact with the interferon gamma activation site in response to multiple cytokines

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2063-2071 ◽  
Author(s):  
P Lamb ◽  
LV Kessler ◽  
C Suto ◽  
DE Levy ◽  
HM Seidel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Interferon Gamma ◽  
Regulation Of Gene Expression ◽  
Interleukin 2 ◽  
Sequence Element ◽  
Ifn Gamma ◽  
Gm Csf ◽  
Rapid Changes ◽  
Rapid Activation

Many cytokines and growth factors trigger rapid changes in gene expression upon binding to their receptors. In many cases, the mechanism by which these changes are affected is unknown. In this report, we show that interleukin-2 (IL-2), IL-3, IL-4, IL-6, leukemia inhibitory factor (LIF), erythropoietin (Epo), and granulocyte- macrophage colony-stimulating factor (GM-CSF) treatment of cells causes rapid activation of DNA-binding activities that recognize a DNA sequence element previously implicated in regulation of gene expression by interferon gamma (IFN gamma). The IL-4-, IL-6-, and GM-CSF-induced complexes can be distinguished from the recently characterized IFN gamma-activated protein p91 on the basis of mobility in polyacrylamide gels, sequence preferences, and lack of reactivity with an anti-p91 antiserum. The IL-4- and GM-CSF-induced complexes react with antiphosphotyrosine antibodies, demonstrating the presence of phosphotyrosine-containing proteins in these DNA-binding complexes. Transcriptional activation of a reporter gene linked to a synthetic IFN gamma-responsive promoter is observed in response to IFN gamma, IL-6, and LIF. These data suggest a pathway by which cytokines induce rapid changes in gene expression.

scholarly journals Cooperative Activation of Human Papillomavirus Type 8 Gene Expression by the E2 Protein and the Cellular Coactivator p300

2002 ◽  
Vol 76 (21) ◽  
pp. 11042-11053 ◽  
Author(s):  
Andreas Müller ◽  
Andreas Ritzkowsky ◽  
Gertrud Steger
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Specific Binding ◽  
Regulatory Region ◽  
Keratinocyte Differentiation ◽  
E2 Protein ◽  
Protein Protein Interaction ◽  
Cooperative Activation

ABSTRACT The E2 proteins of papillomaviruses (PV) bind to the coactivator CBP/p300 as do many other transcription factors, but the precise role of CBP/p300 in E2-specific functions is not yet understood. We show that the E2 protein of human PV type 8 (HPV8) directly binds to p300. Activation of HPV8 gene expression by low amounts of HPV8 E2 was stimulated up to sevenfold by coexpression of p300. The interaction between E2 and p300 may play a role in differentiation-dependent activation of PV gene expression, since we can show that the expression level of p300 increases during keratinocyte differentiation. Surprisingly, sequence-specific binding of E2 to its recognition sites within the regulatory region of HPV8 is not necessary for this cooperation, indicating that E2 can be recruited to the promoter via protein-protein interaction. HPV8 E2 binds via its N-terminal activation domain (AD), its C-terminal DNA binding domain (DBD), and its internal hinge region to p300 in vitro. Transient-transfection assays revealed that the AD is necessary and sufficient for cooperative activation with p300. However, we provide evidence that the interaction of the hinge and the DBD of HPV8 E2 with p300 may contribute. Our data suggest an important role of p300 in regulation of HPV8 gene expression and reveal a new mechanism by which E2 may be recruited to a promoter to activate transcription without sequence specific DNA binding.

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