Thrombin attenuates the stimulatory effect of histamine on Ca2+ entry in confluent human umbilical vein endothelial cell cultures

The effect of adenosine on proliferation of human endothelial cells was investigated by adding adenosine to the medium of cultures derived from human umbilical veins. Cell counts on cultures grown in 10 microM adenosine for 4–7 days were 41–53% greater than counts from control cultures. In contrast, 10 microM adenosine had no effect on growth of a human fibroblast cell strain (IMR-90). Neither inosine nor 2',5'-dideoxyadenosine influenced endothelial cell growth at concentrations of 0.1 or 10 microM. Addition of adenosine deaminase abolished the proliferative effect of added adenosine and inhibited proliferation by 16% in control cultures, suggesting that endogenous adenosine may enhance proliferation in culture. The adenosine receptor antagonist, 8-phenyltheophylline, at 0.1 and 1.0 microM blocked the enhanced proliferation caused by 10 microM adenosine. Addition of 10 microM adenosine enhanced DNA synthesis in endothelial cell cultures as indicated by an increased incorporation of [3H]thymidine into acid-insoluble cell material. The results indicate that addition of physiological concentrations of adenosine to human umbilical vein endothelial cell cultures stimulates proliferation, possibly via a surface receptor, and suggest that adenosine may be a factor for human endothelial cell growth and possibly angiogenesis.

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Self-regulation of procoagulant events on the endothelial cell surface.

Journal of Experimental Medicine ◽

10.1084/jem.162.4.1223 ◽

1985 ◽

Vol 162 (4) ◽

pp. 1223-1235 ◽

Cited By ~ 135

Author(s):

D M Stern ◽

I Bank ◽

P P Nawroth ◽

J Cassimeris ◽

W Kisiel ◽

...

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Tissue Factor ◽

Cell Cultures ◽

Umbilical Vein ◽

Interleukin 1 ◽

Self Regulation ◽

Procoagulant Activity ◽

Dependent Manner ◽

Endothelial Cell Cultures

Interleukin 1 (IL-1) is a potent mediator of inflammatory and immunologic phenomena. In addition, IL-1 may be intimately involved in the regulation of hemostasis, since interaction of IL-1 with endothelial cells has been reported to induce tissue factor activity. We demonstrate that perturbation of the endothelial cell induces augmented IL-1 release. Human umbilical vein endothelial cells perturbed by treatment with lipopolysaccharide produced enhanced amounts of IL-1 activity. IL-1 activity from lipopolysaccharide-treated endothelial cell supernatants could be absorbed by an antibody to IL-1 coupled to Sepharose. Elaboration of IL-1 activity was dependent on the dose of lipopolysaccharide and occurred in a time-dependent manner. Addition of cycloheximide blocked generation of IL-1 activity. A physiological vessel wall perturbant, the coagulation enzyme thrombin, induced comparable amounts of IL-1 activity in endothelial cell cultures. This effect was specific for the enzyme, since active site-blocked thrombin and prothrombin had no effect on IL-1. In addition, IL-1-containing supernatants from thrombin-stimulated endothelial cells induced tissue factor procoagulant activity in fresh endothelial cell cultures. Thus, in contrast to the multiple, known inhibitory mechanisms that block thrombin procoagulant activity, these data suggest a circle of interaction in which thrombin induces endothelial cell elaboration of IL-1, a mediator of endothelial cell procoagulant activity. Endothelial cell production of IL-1 in response to perturbation allows these cells to play an integral role in the regulation of the inflammatory and coagulation systems.

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Effect of Ahthocyanosides and Dexamethasone on Cultured Cells from Huilan Umbilical Vein

10.1055/s-0039-1687613 ◽

1979 ◽

Author(s):

F. Piovella ◽

M. Pesenti ◽

M.M. Ricetti ◽

C. Castasola ◽

P.L. Alnasio ◽

...

Keyword(s):

Endothelial Cell ◽

Protein Content ◽

Cell Cultures ◽

Cultured Cells ◽

Umbilical Vein ◽

Cell Spreading ◽

Cell Type ◽

Endothelial Cell Cultures ◽

Positive Effect ◽

Cellular Types

The effects of Vaccinium myrtillue anthocyanosides, substances which demonstrated significant effect on the formation of new capillaries in experimental animals, and of dexamethasone were evaluated on cell cultures derived from umbilical vein. According to Shearn et al, pure endothelial cell suspensions were grown as primary and passed cell cultures. In the same way suspensions of highly contaminated specimens containing both ri broblasts and smooth muscle cells together with endothelial cells were cultured. Both types of culture were exposed to different concentrations of the two stimulants. In pure endothelial cell cultures, dexamethasone enhanced cell spreading as well as protein content. Anthocyanosides demonstrated a positive effect on cell proliferation and protein content. In different cell type cultures, dexamethasone stimulated the proliferation of endothelium more actively than other cells, while anthocyanoside s showed a stimulating activity on all different cellular types.

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