Magnesol thin-layer chromatography of flavonoid compounds

1969 ◽  
Vol 43 ◽  
pp. 287-290 ◽  
Author(s):  
R.L. Larson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Flavonoid Compounds ◽  
Layer Chromatography

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN MAHONI (Swietenia mahagoni (L.) Jacq.) TERHADAP LARVA Aedes aegypti L.

2017 ◽  
Vol 4 (2) ◽  
pp. 23
Author(s):  
Tisa Rizkika Nur Amelia
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Key Words ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Botanical Insecticide ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Swietenia Mahagoni

<p>The aim of the research were to evaluate the efficacy of botanical insecticide of <em>S. mahagoni </em>leaves extracts against larvae of <em>Ae. aegypti,</em> based on concentration of the leaves <em>S. mahagoni </em>extract, and in additional to determine secondary metabolites compounds of <em>S. mahagoni </em>leaves extract. The extraction of <em>S. mahagoni </em>leaves was done by using ethanol solvents and than was analyzed by using Thin Layer Chromatography. The result indicated that ethanolic extract of <em>S. mahagoni </em>leaf contained alkaloid, tannin, saponin, terpenoid, and flavonoid compounds. The value of LC<sub>50 </sub>and LC<sub>90</sub> calculation showed that LC<sub>50</sub> of ethanolic extract over second and third instar larvae respectively were 488 ppm and 644 ppm. However the value of LC<sub>90</sub> of both instar larvae were 732 ppm and 797 ppm. Based on the above result, it can be concluded that ethanolic extract of <em>S. mahagoni </em>leaf was effective against larvae of <em>Ae .aegypti</em>.</p><p>Key words: <em>Ae. aegypti</em>, <em>S. mahagoni</em>, botanical insecticide</p>

scholarly journals Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves

2020 ◽  
Vol 23 (2) ◽  
pp. 81
Author(s):  
Megawati Megawati ◽  
Akhmad Darmawan ◽  
Sofa Fajriah
Keyword(s):  
Thin Layer ◽  
Cytotoxic Activity ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Assay Method ◽  
Cytotoxic Activities ◽  
Spectroscopic Techniques ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Mcf 7

Tetrameles nudliflora is one of the endemic plants in Wallace region, especially in Mekongga Forest, Southeast Sulawesi, Indonesia. As part of our project in the field of natural drug discovery based on local bioresources, this study aimed to isolate secondary metabolite compounds from the ethyl acetate fractions of the leaves of T. nudiflora and evaluate their cytotoxic activities against breast carcinoma cancer lines, MCF-7. The methods used were various chromatography techniques such as vacuum column chromatography, gravitation column chromatography, thin layer chromatography, preparative thin layer chromatography, and re-crystallization using two different organic solvents. In the present study, two flavonoid compounds, kaempferol (1) and quercetin (2) were identified. The structures of 1-2 were determined using serial spectroscopic techniques, including mass spectrometry, one- and two-dimensional nuclear magnetic resonance. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay method, both compounds showed in vitro cytotoxic activity IC50 of 34.162 and 133.148 μg mL-1, respectively against MCF-7 cell lines.

scholarly journals Isolation and identification of flavonoid compounds from Curcuma’s rhizome (Curcuma aeruginosa Roxb.)

2005 ◽  
Vol 3 (1) ◽  
pp. 32-38
Author(s):  
KHOIRINA DWI NUGRAHANINGTYAS ◽  
SABIRIN MATSJEH ◽  
TUTIK DWI WAHYUNI
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Color Test ◽  
Isolation And Identification ◽  
Single Fraction ◽  
Flavonoid Compounds ◽  
Soxhlet Method ◽  
Layer Chromatography ◽  
Test Result

This research was aimed to isolate and identify the flavonoid compounds from curcuma’ rhizome (Curcuma aeruginosa Roxb: Zingiberaceae). The extraction was carried out by Soxhlet method using petroleum eter, chloroform, nbutanol and methanol as the solvent agent. Those extract then qualitatively tested to identify the presence of flavonoid. Flavonoid then isolated from the extract of petroleum eter by column chromatography. The fraction resulted from chromatographic was analyzed by thin layer chromatography (TLC) method. Flavonoid identification was tested by color test, spectrophotometer UV-Vis, IR and GC-MS. The color test result showed that extract of petroleum eter, chloroform and n-butanol contain flavonoid. The analyzed single fraction from column chromatography showed that f2 contains isoflavone with 2 methoxy and 1 ethyl substitutes, f4 contains isoflavone with 2 methoxy substitutes, then f9 contains isoflavone with 1 hydroxy and 2 methoxy substitutes.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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