scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN MAHONI (Swietenia mahagoni (L.) Jacq.) TERHADAP LARVA Aedes aegypti L.

2017 ◽  
Vol 4 (2) ◽  
pp. 23
Author(s):  
Tisa Rizkika Nur Amelia
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Key Words ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
Botanical Insecticide ◽  
Flavonoid Compounds ◽  
Layer Chromatography ◽  
Swietenia Mahagoni

<p>The aim of the research were to evaluate the efficacy of botanical insecticide of <em>S. mahagoni </em>leaves extracts against larvae of <em>Ae. aegypti,</em> based on concentration of the leaves <em>S. mahagoni </em>extract, and in additional to determine secondary metabolites compounds of <em>S. mahagoni </em>leaves extract. The extraction of <em>S. mahagoni </em>leaves was done by using ethanol solvents and than was analyzed by using Thin Layer Chromatography. The result indicated that ethanolic extract of <em>S. mahagoni </em>leaf contained alkaloid, tannin, saponin, terpenoid, and flavonoid compounds. The value of LC<sub>50 </sub>and LC<sub>90</sub> calculation showed that LC<sub>50</sub> of ethanolic extract over second and third instar larvae respectively were 488 ppm and 644 ppm. However the value of LC<sub>90</sub> of both instar larvae were 732 ppm and 797 ppm. Based on the above result, it can be concluded that ethanolic extract of <em>S. mahagoni </em>leaf was effective against larvae of <em>Ae .aegypti</em>.</p><p>Key words: <em>Ae. aegypti</em>, <em>S. mahagoni</em>, botanical insecticide</p>

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

scholarly journals Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

2014 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Rahmawati Ekaputri ◽  
Sudarsono Sudarsono ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Fruit Extract ◽  
Vinca Rosea ◽  
Layer Chromatography

<p>Background: <em>Vinca rosea</em> is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of <em>Vinca rosea</em> was selected to investigation its larvicidal activity against the 3<sup>rd</sup> instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) <em>Aedes aegypti.</em>  Five concentrations of Vinca fruit extract were tested against the 3<sup>rd</sup> instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC<sub>50</sub> anf LC<sub>90</sub>) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC<sub>50</sub> and LC<sub>90</sub> values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.</p>

TELAAH FITOKIMIA KULIT KACANG TANAH (Arachis hypogaea, L).

2021 ◽  
Vol 10 (1) ◽  
pp. 82-91
Author(s):  
Rizki Nisfi Ramdhini ◽  
Isna Mulyani ◽  
Syaikhul Aziz
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Waste Product ◽  
Phytochemical Screening ◽  
Medicinal Products ◽  
Processing Industry ◽  
Arachis Hypogaea L ◽  
Tlc Analysis ◽  
Layer Chromatography

Peanut peel are a waste product of the peanut processing industry with little commercial value. Some of studies have been conducted indicating peanut peel can be beneficial as a source for traditional medicinal products since it is also rich of antioxidants. The aim of this research was to identify the content of secondary metabolites on the peanut peel. The method used was maseration with 96% ethanol. Phytochemical screening and assaying were performed using thin layer chromatography (TLC) method. The results of TLC analysis showed that the secondary metabolites in peanut peel were positive for flavonoids, alkaloids, tannins and quinon. Keywords: Peanut peel, Phytochemical, Thin-Layer chromatography (TLC)

Toxin-producing fungi on feed grains and application of yeasts for their detoxification

2013 ◽  
Vol 16 (2) ◽  
pp. 391-393 ◽  
Author(s):  
J. Repečkienė ◽  
L. Levinskaitė ◽  
A. Paškevičius ◽  
V. Raudonienė
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Rate ◽  
Toxic Compounds ◽  
Fungal Strains ◽  
Yeast Strains ◽  
Grain Contamination ◽  
Layer Chromatography ◽  
Fungal Secondary Metabolites

Abstract The research deals with the contamination of feeding crops with fungi, producing mycotoxins and use of selected yeasts for detoxification. The thin-layer chromatography and ELISA methods were applied for the analysis of fungal secondary metabolites. The results showed a high rate of the grain contamination with fungi, mainly from genera Penicillium, Fusarium, Aspergillus and Alternaria. Some of the fungal strains produced 6-9 toxic compounds. The novelty of the study was the application of the yeast strains in detoxification of aflatoxins, zearalenon and deoxynivalenol in feed.

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