Increasing extraction efficiency in supercritical fluid extraction from complex matrices Predicting extraction efficiency of diuron and linuron in supercritical fluid extraction using supercritical fluid chromatographic retention

1988 ◽  
Vol 447 (3) ◽  
pp. 53-63 ◽  
Author(s):  
M McNally
2008 ◽  
Vol 56 (13) ◽  
pp. 5153-5157 ◽  
Author(s):  
Jessica L. Yee ◽  
John Walker ◽  
Hany Khalil ◽  
Rafael Jiménez-Flores

2002 ◽  
Vol 90 (3) ◽  
Author(s):  
R. Kumar ◽  
N. Sivaraman ◽  
T. G. Srinivasan ◽  
P. R. Vasudeva Rao

SummaryExtraction of uranium from tissue matrix was studied using supercritical carbon dioxide containing modifier solvent. The extraction efficiency was investigated with carbon dioxide containing methanol, tri-


2008 ◽  
Vol 96 (12) ◽  
Author(s):  
Ankita Rao ◽  
Pradeep Kumar ◽  
K. L. Ramakumar

AbstractIn the supercritical fluid extraction of uranium from acidic medium employing TBP as co-solvent, effects of various parameters on extraction efficiency were studied. Variation in pressure (80–300 atm), temperature (308–353 K), CO


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Avinash Singh Patel ◽  
Abhijit Kar ◽  
Sukanta Dash ◽  
Sanjaya K. Dash

AbstractStudy ascertained the recovery of β-carotene from enzyme-treated (enzyme load of 167 U/g) pericarp of ripe bitter melon using supercritical fluid extraction (SFE) technique. Effect of different pressure (ranged from 150–450 bar), carbon dioxide (CO2) flow rates (ranged from 15 to 55 ml/min), temperatures (from 50 to 90 °C), and extraction periods (from 45–225 minutes) were observed on the extraction efficiency of β-carotene. Results showed that extraction pressure (X1) among extraction parameters had the most significant (p < 0.05) effect on extraction efficiency of the β-carotene followed by allowed extraction time (X4), CO2 flow rate (X2) and the temperature of the extraction (X3). The maximum yield of 90.12% of β-carotene from lyophilized enzymatic pretreated ripe bitter melon pericarp was achieved at the pressure of approx. 390 bar, flow rate of 35 mL/min, temperature at 70 °C and extraction time of 190 min, respectively. Based on the accelerated storage study the 70% retention shelf life of the β-carotene into extract was estimated up to 2.27 months at 10 °C and up to 3.21 months at 5 °C.


2020 ◽  
Author(s):  
Chantal Bader ◽  
Markus Neuber ◽  
Fabian Panter ◽  
Daniel Krug ◽  
Rolf Müller

Supercritical fluid extraction (SFE) is widely used for the isolation of natural products from plants, but its application in efforts to identify structurally and physicochemically often dissimilar microbial natural products is limited to date. In this study we evaluated the impact of SFE on the extractability of myxobacterial secondary metabolites aiming to improve the prospects of discovering novel natural products. We investigated the influence of different co-solvents on the extraction efficiency of secondary metabolites from three myxobacterial strains as well as the antimicrobial activity profiles of the corresponding extracts. For each known secondary metabolite we found extraction conditions using SFE leading to superior yields in the extracts compared to conventional solvent extraction. Compounds with a logP higher than 3 showed best extraction efficiency using 20% EtOAc as a co-solvent, whereas compounds with logP values lower than 3 were better extractable using more polar co-solvents like MeOH. Extracts generated with SFE showed increased antimicrobial activities including the presence of activities not explained by known myxobacterial secondary metabolites, highlighting the advantage of SFE for bioactivity-guided isolation. Moreover, non-targeted metabolomics analysis revealed a group of chlorinated metabolites produced by the well-studied model myxobacterium Myxococcus xanthus DK1622 which were not accessible previously due to their low concentration in conventional extracts. The enriched SF extracts were used for isolation and subsequent structure elucidation of chloroxanthic acid A as founding member of a novel secondary metabolite family. Our findings encourage the increased utilization of SFE as part of future microbial natural products screening workflows.


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