Studies on growth and flowering in axenic cultures of insectivorous plants: III. Effects of photoperiod, ethrel, morphactin and a few other growth substances and metabolic inhibitors on Utricularia inflexa

Several algal types were tested for the ability to assimilate a variety of organic compounds including glucose, sucrose, glycerol, acetate, and a variety of amino acids. Axenic cultures of Phaeodactylum tricornutum, Cricosphaera sp., and Dunaliella tertiolecta failed to take up any of the compounds tested. Axenic cultures of the filamentous blue-green algae Phormidium sp. and Lyngbya sp. took up all of the test substrates, as did the "olive-green cells" (a non-bacteria-free sample of marine algal cells described as olive-green cells by other workers). The results of experiments to determine uptake rates over the range 10−7 to 10−3 molar substrate, rates of uptake at 18, 24, and 32C, and rates of uptake in the presence of the metabolic inhibitors dinitrophenol (DNP) and carbanyl cyanide m-chlorophenylhydrazone (CCCP) indicated that uptake of the organic compounds tested by the filamentous blue-green algae tested is not by an active transport mechanism.

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Metabolism of excised embryos of Lupinus luteus L. I. Effect of metabolic inhibitors and growth substances on the water uptake

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1962.012 ◽

2015 ◽

Vol 31 (1) ◽

pp. 135-152 ◽

Cited By ~ 3

Author(s):

J. Czosnowski

Keyword(s):

Water Uptake ◽

Lupinus Luteus ◽

Metabolic Inhibitors ◽

Growth Substances ◽

Excised Embryos

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Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066796 ◽

1971 ◽

Vol 29 ◽

pp. 548-549

Author(s):

Awtar Krishan ◽

Dora Hsu

Keyword(s):

Granular Material ◽

Rna Synthesis ◽

Culture Medium ◽

Actinomycin D ◽

Metabolic Inhibitors ◽

Protein And Rna Synthesis ◽

Apparent Reduction ◽

Plant Alkaloids ◽

In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

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Peroxidase Localization in Hartmanella Trophozoites

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065778 ◽

1971 ◽

Vol 29 ◽

pp. 346-347 ◽

Cited By ~ 1

Author(s):

George E. Childs ◽

Joseph H. Miller

Keyword(s):

Hydrogen Peroxide ◽

Ultrastructural Localization ◽

Pathogenic Strain ◽

Oxidative Enzymes ◽

Differential Centrifugation ◽

Electron Microscopic ◽

Microscopic Studies ◽

The Subject ◽

Axenic Cultures

Biochemical and differential centrifugation studies have demonstrated that the oxidative enzymes of Acanthamoeba sp. are localized in mitochondria and peroxisomes (microbodies). Although hartmanellid amoebae have been the subject of several electron microscopic studies, peroxisomes have not been described from these organisms or other protozoa. Cytochemical tests employing diaminobenzidine-tetra HCl (DAB) and hydrogen peroxide were used for the ultrastructural localization of peroxidases of trophozoites of Hartmanella sp. (A-l, Culbertson), a pathogenic strain grown in axenic cultures of trypticase soy broth.

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Insectivorous Plants

10.1017/cbo9780511694127 ◽

2009 ◽

Cited By ~ 1

Author(s):

Charles Darwin

Keyword(s):

Insectivorous Plants

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Platelet Aggregation Caused by Dithiothreitol

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661036 ◽

1984 ◽

Vol 51 (01) ◽

pp. 119-124 ◽

Cited By ~ 59

Author(s):

M B Zucker ◽

N C Masiello

Keyword(s):

Shape Change ◽

Blood Platelets ◽

Dense Granule ◽

Metabolic Inhibitors ◽

Electrophoretic Patterns ◽

Discernible Effect ◽

Variable Extent ◽

Triton X ◽

Induced Aggregation ◽

Platelet Shape

SummaryMacIntyre et al. showed that over 1 mM dithiothreitol (DTT) aggregates blood platelets in the presence of fibrinogen; aggregation is not inhibited by prostaglandin E1. We confirmed their data and found that 70 mM 2-mercaptoethanol was also active. DTT- induced aggregation was not associated with platelet shape change or secretion of dense granule contents, was not inhibited by tetracaine or metabolic inhibitors, was prevented at pH 6.5, and prevented, reversed, or arrested by EDTA, depending on when the EDTA was added. DTT did not cause aggregation of thrombasthenic, EDTA-treated, or cold (0° C) platelets, which also failed to aggregate with ADP. Platelets stimulated with DTT bound 125I-labeled fibrinogen. Thus DTT appears to “expose” the fibrinogen receptors. SDS gel electrophoresis of platelet fractions prepared by use of Triton X-114 showed that aggregating concentrations of DTT reduced proteins of apparent Mr 69,000 and 52,000 (probably platelet albumin) and, to a variable extent, glycoproteins Ib, IIb and III. Exposure of unlabeled or 125I- labeled platelets to ADP had no discernible effect on the electrophoretic patterns.

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