Alterations of P19ARF in Rodent Hepatoma Cell Lines but not in Human Primary Liver Cancer

2000 ◽  
Vol 117 (2) ◽  
pp. 118-124 ◽  
Author(s):  
Jean-François Laes ◽  
Luis Antonio Parada ◽  
Bertil Johansson ◽  
Göran Levan ◽  
Claude Szpirer ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Primary Liver Cancer ◽  
Hepatoma Cell ◽  
Hepatoma Cell Lines

scholarly journals Heparin and Liver Heparan Sulfate Can Rescue Hepatoma Cells from Topotecan Action

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
József Dudás ◽  
József Bocsi ◽  
Alexandra Fullár ◽  
Kornélia Baghy ◽  
Tibor Füle ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Heparan Sulfate ◽  
Cell Lines ◽  
Topoisomerase I ◽  
Hepatoma Cell ◽  
Normal Liver ◽  
Hepatoma Cell Lines ◽  
Inhibitory Compound ◽  
Hep3b Cells

Topotecan (TpT) is a major inhibitory compound of topoisomerase (topo) I that plays important roles in gene transcription and cell division. We have previously reported that heparin and heparan sulfate (HS) might be transported to the cell nucleus and they can interact with topoisomerase I. We hypothesized that heparin and HS might interfere with the action of TpT. To test this hypothesis we isolated topoisomerase I containing cell nuclear protein fractions from normal liver, liver cancer tissues, and hepatoma cell lines. The enzymatic activity of these extracts was measured in the presence of heparin, liver HS, and liver cancer HS. In addition, topo I activity, cell viability, and apoptosis of HepG2 and Hep3B cells were investigated after heparin and TpT treatments. Liver cancer HS inhibited topo I activity in vitro. Heparin treatment abrogated topo I enzyme activity in Hep3B cells, but not in HepG2 cells, where the basal activity was higher. Heparin protected the two hepatoma cell lines from TpT actions and decreased the rate of TpT induced S phase block and cell death. These results suggest that heparin and HS might interfere with the function of TpT in liver and liver cancer.

The Novel Target of Liver Cancer: MicroRNA-4324 Regulates Cell Proliferation and Migration via Targeting Neuraminidase 3

2021 ◽  
Vol 11 (5) ◽  
pp. 920-928
Author(s):  
Ruiyuan Jiang ◽  
Jiacheng Xie ◽  
Xiaohua Hong ◽  
Tingting Man ◽  
Mengna Yang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Cancer ◽  
Cell Lines ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Hepatoma Cell ◽  
Hepatoma Cells ◽  
Migration And Invasion ◽  
Hepatoma Cell Lines ◽  
And Migration

Background: MicroRNA-4324 has been reported to regulate various biological malignant cancer. Nonetheless, the expression and molecular mechanism of miR-4324 in liver cancer remain rarely known. This study aimed to investigate the effect miR-4324 on the proliferation, invasion and migration of hepatoma cells. Methods: The mRNA level of miR-4324 was assessed in four hepatoma cell lines (HepG2, Huh7, MHCC97, HB611) and human embryonic liver cell, HHL5. MiR-4324 was over-expressed in hepatoma cells. Subsequently, the effects of miR-4324 on cell proliferation, migration and invasion and the underlying molecular mechanisms were detected. Results: Our data indicated that miR-4324 was down-regulation in hepatoma cell lines compared with HHL5. Overexpression of miR-4324 inhibits cellular proliferation, colony-formation, migration and invasion abilities of hepatoma cells. However, the biological effects of miR-4324 overexpression on hepatoma cells were reversed after overexpressing NEU3. Conclusions: Our findings concluded that miR-4324 inhibits biological functions of hepatoma cells by targeting NEU3 and it might be a potential target for the treatment of liver cancer.

scholarly journals Multiplexed Proteomic Approach for Identification of Serum Biomarkers in Hepatocellular Carcinoma Patients with Normal AFP

2020 ◽  
Vol 9 (2) ◽  
pp. 323
Author(s):  
Young-Sun Lee ◽  
Eunjung Ko ◽  
Eileen L. Yoon ◽  
Young Kul Jung ◽  
Ji Hoon Kim ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Cell Lines ◽  
Cellular Proliferation ◽  
Hepatoma Cell ◽  
Multiple Reaction Monitoring ◽  
Serum Levels ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Proteomic Approach

Alpha fetoprotein (AFP) has been used as a serologic indicator of hepatocellular carcinoma (HCC). We aimed to identify an HCC-specific serum biomarker for diagnosis using a multiplexed proteomic technique in HCC patients with normal AFP levels. A total of 152 patients were included from Guro Hospital, Korea University. Among 267 identified proteins, 28 and 86 proteins showed at least a two-fold elevation or reduction in expression, respectively. Multiple reaction monitoring (MRM) analysis of 41 proteins revealed 10 proteins were differentially expressed in patients with liver cirrhosis and HCC patients with normal AFP. A combination of tripartite motif22 (Trim22), seprase, and bone morphogenetic protein1 had an area under receiver operating characteristic of 0.957 for HCC diagnosis. Real-time PCR and western blot analysis of the paired tumor/non-tumor liver tissue in HCC revealed a reduced expression of Trim22 in the tumor tissue. Also, serum levels of Trim22 were significantly reduced in HCC patients with normal AFP compared to those with liver cirrhosis (p = 0.032). Inhibition of Trim22 increased cellular proliferation in human hepatoma cell lines, whereas overexpression of Trim22 decreased cellular proliferation in hepatoma cell lines. In conclusion, the combination of three serum markers improved the chance of diagnosing HCC. MRM-based quantification of the serum protein in patients with normal AFP provides the potential for early diagnosis of HCC.

scholarly journals The Limonoids TS3 and Rubescin E Induce Apoptosis in Human Hepatoma Cell Lines and Interfere with NF-κB Signaling

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160843 ◽  
Author(s):  
Nicole Lange ◽  
Armelle Tsamo Tontsa ◽  
Claudia Wegscheid ◽  
Pierre Mkounga ◽  
Augustin Ephrem Nkengfack ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines
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