Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs

2000 ◽  
Vol 43 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Tom Lewis ◽  
Peter D Nichols ◽  
Tom A McMeekin
Keyword(s):  
Fatty Acids ◽  
Extraction Methods

Determination of fatty acids from Spirulina platensis under different extraction methods

2013 ◽  
Vol 24 ◽  
pp. S91-S92
Author(s):  
Oya Irmak Sahin ◽  
Arzu Akpınar Bayizit ◽  
Belkıs Canan
Keyword(s):  
Fatty Acids ◽  
Spirulina Platensis ◽  
Extraction Methods

The effects of different extraction methods of lipids from Nannochloropsis oceanica on the contents of omega-3 fatty acids

2019 ◽  
Vol 41 ◽  
pp. 101556 ◽  
Author(s):  
Ana R.P. Figueiredo ◽  
Elisabete da Costa ◽  
Joana Silva ◽  
M. Rosário Domingues ◽  
Pedro Domingues
Keyword(s):  
Fatty Acids ◽  
Extraction Methods ◽  
Nannochloropsis Oceanica ◽  
Omega 3 Fatty Acids ◽  
Omega 3

scholarly journals Advanced Extraction of Lipids with DHA from Isochrysis galbana with Enzymatic Pre-Treatment Combined with Pressurized Liquids and Ultrasound Assisted Extractions

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3310
Author(s):  
María Señoráns ◽  
Natalia Castejón ◽  
Francisco Javier Señoráns
Keyword(s):  
Fatty Acids ◽  
Isochrysis Galbana ◽  
Extraction Methods ◽  
Polar Lipids ◽  
Omega 3 ◽  
Extraction Techniques ◽  
Advanced Technique ◽  
Microalgal Biomass ◽  
Ultrasound Assisted ◽  
Pre Treatment

Microalgal biomass is a sustainable and valuable source of lipids with omega-3 fatty acids. The efficient extraction of lipids from microalgae requires fast and alternative extraction methods, frequently combined with biomass pre-treatment by different procedures. In this work, Pressurized liquid extraction (PLE) was optimized and compared with traditional lipid extraction methods, Folch and Bligh and Dyer, and with a new Ultrasound Assisted Extraction (UAE) method for lipids from microalgae Isochrysis galbana. To further optimize PLE and UAE, enzymatic pre-treatment of microalga Isochrysis galbana was studied with commercial enzymes Viscozyme and Celluclast. No significant differences were found for lipid yields among different extraction techniques used. However, advanced extraction techniques with or without pre-treatment are a green, fast, and toxic solvent free alternative to traditional techniques. Lipid composition of Isochrysis was determined by HPLC-ELSD and included neutral and polar lipids, showing that each fraction comprised different contents in omega-3 polyunsaturated fatty acids (PUFA). The highest polar lipids content was achieved with UAE (50 °C and 15 min) and PLE (100 °C) techniques. Moreover, the highest omega-3 PUFA (33.2%), eicosapentaenoic acid (EPA) (3.3%) and docosahexaenoic acid (DHA) (12.0%) contents were achieved with the advanced technique UAE, showing the optimized method as a practical alternative to produce valuable lipids for food and nutraceutical applications.

scholarly journals Innovative and Conventional Valorizations of Grape Seeds from Winery By-Products as Sustainable Source of Lipophilic Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 568
Author(s):  
Ivana Dimić ◽  
Nemanja Teslić ◽  
Predrag Putnik ◽  
Danijela Bursać Kovačević ◽  
Zoran Zeković ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oil Recovery ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Tocopherol Content ◽  
Industrial Applications ◽  
Operating Conditions ◽  
Vitis Vinifera L ◽  
Grape Seeds ◽  
Extraction Technology

The aim of this study was to valorize the oil recovery from red and white grape seeds (Vitis vinifera L.) that remains as by-product after the winemaking process. Oils were extracted by modern techniques, ultrasound assisted (UAE), microwave assisted (MAE) and supercritical fluid extraction (SFE), and compared to the Soxhlet extraction (SE). Firstly, SFE was optimized at different operating conditions: pressure (250–350 bar), temperature (40–60 °C), CO2 flow rate (0.2, 0.3 and 0.4 kg h−1), and particle size (315–800 µm and >800 µm). The highest extraction yields were achieved by SFE at the optimal conditions: 350 bar, 60 °C, 0.4 kg h−1. Afterwards, SFE was compared to SE, UAE and MAE with respect to oil extraction yields, and analyzed for fatty acid composition and antioxidant capacity. Considering the general classification of fatty acids, it was found that samples had high content of polyunsaturated fatty acids, regardless of extraction technology. Tocopherol content was significantly influenced by all extraction methods, whereas UAE and MAE resulted in extracts richer with lipophilic antioxidants. In conclusion, modern extractions that are suited for industrial applications had better performance as compared to SE, as judging by the oil yield and quality.

scholarly journals Chia Seeds (Salvia Hispanica L.): An Overview—Phytochemical Profile, Isolation Methods, and Application

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 11 ◽  
Author(s):  
Maša Knez Hrnčič ◽  
Maja Ivanovski ◽  
Darija Cör ◽  
Željko Knez
Keyword(s):  
Fatty Acids ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Herbaceous Plant ◽  
Salvia Hispanica ◽  
Phytochemical Profile ◽  
Salvia Hispanica L ◽  
Fiber Proteins ◽  
Excellent Source ◽  
Chia Oil

Chia (Salvia hispanica L.) is a small seed that comes from an annual herbaceous plant, Salvia hispanica L. In recent years, usage of Chia seeds has tremendously grown due to their high nutritional and medicinal values. Chia was cultivated by Mesopotamian cultures, but then disappeared for centuries until the middle of the 20th century, when it was rediscovered. Chia seeds contain healthy ω-3 fatty acids, polyunsaturated fatty acids, dietary fiber, proteins, vitamins, and some minerals. Besides this, the seeds are an excellent source of polyphenols and antioxidants, such as caffeic acid, rosmarinic acid, myricetin, quercetin, and others. Today, chia has been analyzed in different areas of research. Researches around the world have been investigating the benefits of chia seeds in the medicinal, pharmaceutical, and food industry. Chia oil is today one of the most valuable oils on the market. Different extraction methods have been used to produce the oil. In the present study, an extensive overview of the chemical composition, nutritional properties, and antioxidant and antimicrobial activities, along with extraction methods used to produce chia oil, will be discussed.

scholarly journals ENERGY ANALYSIS OF LIPID EXTRACTION OF Scenedesmus sp. PRODUCED IN PILOT SCALE

2015 ◽  
Vol 14 (1) ◽  
pp. 22
Author(s):  
L. Schroeder ◽  
M. D. Scherer ◽  
W. Balmant ◽  
A. B. Mariano ◽  
J. V. C. Vargas
Keyword(s):  
Fatty Acids ◽  
Conversion Rate ◽  
Large Scale ◽  
Classical Method ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Pilot Scale ◽  
Fatty Material ◽  
Energy Usage ◽  
Wet Biomass

he production of biodiesel from lipids extracted from microalgae biomass is a promising approach to biofuels. However, this approach is still not commercialized because of the high costs of processes associated with, for example, time consumption and / or biomass drying with intense energy usage. However, it was not possible to show extraction methods among the lipids existing in the literature, which could be applied specifically to the extraction of lipids from the microalgae Scenedesmus sp. from the large-scale wet biomass, which is the current challenge faced by the Center for Research and Development of Sustainable Energy Auto (NPDEAS). Therefore, in this study, the possibility of avoiding the drying process, and extracting lipids directly from humid biomass, using the saponification method, was tested and compared with conventional Bligh and Dyer extraction (B & D). This study introduced the cultivation of microalgae Scenedesmus sp. compact tubular photobioreactors 12 m3 in area 10 m2 (8 x 5 x 2 m). The classical method of lipid extraction from microalgae - B & D - brings many pigments and polar lipids that exist in the biomass and the conversion rate was only 65-66%, whereas the recovery of fatty material in the wet biomass by the saponification method showed high conversion rate (90-95%). Therefore, the saponification process showed a high recovery of fatty acids that can be easily converted into biodiesel by esterification, and it was shown that the stage of drying the biomass can be removed without losing the fatty acids. In relation to the energy usage in the process, it was shown that drying the biomass for extraction of fatty acids uses more energy than that produced in the final product, biodiesel, showing that the removal of fatty acids of the wet biomass is of strategic importance to the viability of microalgae biodiesel.

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