scholarly journals ENERGY ANALYSIS OF LIPID EXTRACTION OF Scenedesmus sp. PRODUCED IN PILOT SCALE

2015 ◽  
Vol 14 (1) ◽  
pp. 22
Author(s):  
L. Schroeder ◽  
M. D. Scherer ◽  
W. Balmant ◽  
A. B. Mariano ◽  
J. V. C. Vargas
Keyword(s):  
Fatty Acids ◽  
Conversion Rate ◽  
Large Scale ◽  
Classical Method ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Pilot Scale ◽  
Fatty Material ◽  
Energy Usage ◽  
Wet Biomass

he production of biodiesel from lipids extracted from microalgae biomass is a promising approach to biofuels. However, this approach is still not commercialized because of the high costs of processes associated with, for example, time consumption and / or biomass drying with intense energy usage. However, it was not possible to show extraction methods among the lipids existing in the literature, which could be applied specifically to the extraction of lipids from the microalgae Scenedesmus sp. from the large-scale wet biomass, which is the current challenge faced by the Center for Research and Development of Sustainable Energy Auto (NPDEAS). Therefore, in this study, the possibility of avoiding the drying process, and extracting lipids directly from humid biomass, using the saponification method, was tested and compared with conventional Bligh and Dyer extraction (B & D). This study introduced the cultivation of microalgae Scenedesmus sp. compact tubular photobioreactors 12 m3 in area 10 m2 (8 x 5 x 2 m). The classical method of lipid extraction from microalgae - B & D - brings many pigments and polar lipids that exist in the biomass and the conversion rate was only 65-66%, whereas the recovery of fatty material in the wet biomass by the saponification method showed high conversion rate (90-95%). Therefore, the saponification process showed a high recovery of fatty acids that can be easily converted into biodiesel by esterification, and it was shown that the stage of drying the biomass can be removed without losing the fatty acids. In relation to the energy usage in the process, it was shown that drying the biomass for extraction of fatty acids uses more energy than that produced in the final product, biodiesel, showing that the removal of fatty acids of the wet biomass is of strategic importance to the viability of microalgae biodiesel.

scholarly journals Physical Methods of Microalgal Biomass Pretreatment

2014 ◽  
Vol 28 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Agata Piasecka ◽  
Izabela Krzemińska ◽  
Jerzy Tys
Keyword(s):  
Alternative Energy ◽  
Lipid Extraction ◽  
Physical Method ◽  
Extraction Methods ◽  
Biodiesel Production ◽  
Biomass Pretreatment ◽  
Microalgal Biomass ◽  
Alternative Energy Sources ◽  
Wet Biomass ◽  
Microwave Techniques

Abstract The prospect of depletion of natural energy resources on the Earth forces researchers to seek and explore new and alternative energy sources. Biomass is a composite resource that can be used in many ways leading to diversity of products. Therefore, microalgal biomass offers great potential. The main aim of this study is to find the best physical method of microalgal biomass pretreatment that guarantees efficient lipid extraction. These studies identifies biochemical composition of microalgal biomass as source for biodisel production. The influence of drying at different temperatures and lyophilization was investigated. In addition, wet and untreated biomass was examined. Cell disruption (sonication and microwave) techniques were used to improve lipid extraction from wet biomass. Additionally, two different extraction methods were carried out to select the best method of crude oil extraction. The results of this study show that wet biomass after sonication is the most suitable for extraction. The fatty acid composition of microalgal biomass includes linoleic acid (C18:2), palmitic acid (C16:0), oleic acid (C18:1), linolenic acid (C18:3), and stearic acid (C18:0), which play a key role in biodiesel production.

Evaluation of Method-Specific Extraction Variability for the Measurement of Fatty Acids in a Candidate Infant/Adult Nutritional Formula Reference Material

2017 ◽  
Vol 100 (3) ◽  
pp. 814-819
Author(s):  
Benjamin J Place
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Reference Material ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Lipid Extraction ◽  
Acid Methyl Ester ◽  
Extraction Methods ◽  
Community Needs ◽  
Isolated Soy Protein

Abstract To address community needs, the National Institute of Standards and Technology has developed a candidate Standard Reference Material (SRM) for infant/adult nutritional formula based on milk and whey protein concentrates with isolated soy protein called SRM 1869 Infant/Adult Nutritional Formula. One major component of this candidate SRM is the fatty acid content. In this study, multiple extraction techniques were evaluated to quantify the fatty acids in this new material. Extraction methods that were based on lipid extraction followed by transesterification resulted in lower mass fraction values for all fatty acids than the values measured by methods utilizing in situ transesterification followed by fatty acid methyl ester extraction (ISTE). An ISTE method, based on the identifiedoptimal parameters, was used to determine the fatty acid content of the new infant/adult nutritional formula reference material.

scholarly journals Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

2021 ◽  
Author(s):  
Mustafa Hac İsa ◽  
Cansu Metin ◽  
Ertan Ercan ◽  
Yunus Alparslan
Keyword(s):  
Fatty Acids ◽  
Osmotic Shock ◽  
Lipid Extraction ◽  
Extraction Methods ◽  
Cell Disruption ◽  
Health Indices ◽  
Lipid Yield ◽  
Atherogenicity Index ◽  
Total Fatty Acids ◽  
Enzyme Application

In this study, it was investigated to increase the lipid yield of the microalgae Schizochytrium sp., by applying different cell disruption methods. Therefore, acid treatment with HCl, osmotic shock, enzyme applications and ultrasonic homogenizer were tried in this algae species combined with the Bligh and Dyer and Soxhlet methods as an alternative to classical lipid extraction methods. As a result of the study, the highest lipid value (21.72 ± 0.74%) was obtained in enzyme application with Bligh and Dyer method (BDE). The cell disruption processes increased the lipid yield compared to the control groups. The highest PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the highest value was observed in the BDE group. Highest SFA was palmitic acid. Effective results were observed in the Bligh and Dyer applied groups in terms of both total lipid and total fatty acids. In cell disruption methods, particularly in enzyme and HCl extraction, good results were obtained in terms of fatty acids. The highest total fatty acids and the highest lipid content were detected in the Bligh and Dyer enzyme (BDE). Enzyme applications are also advantageous because of being environmentally friendly. Lipid health indices such as n-6/n-3, PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and hypocholesterolemic/hypercholesterolemic ratios (HH) were almost favorable. With this study, an appropriate lipid extraction methods were determined to provide an economical and environmental friendly suggestion for future studies to be used in areas such as food, feed and cosmetics.

Determination of fatty acids from Spirulina platensis under different extraction methods

2013 ◽  
Vol 24 ◽  
pp. S91-S92
Author(s):  
Oya Irmak Sahin ◽  
Arzu Akpınar Bayizit ◽  
Belkıs Canan
Keyword(s):  
Fatty Acids ◽  
Spirulina Platensis ◽  
Extraction Methods

scholarly journals ENVIRONMENTAL POLLUTION BY WASTEWATER FROM BROWN COAL PROCESSING ¬ A REMEDIATION CASE STUDY IN GERMANY

2014 ◽  
Vol 22 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Arndt Wiessner ◽  
Jochen A. Müller ◽  
Peter Kuschk ◽  
Uwe Kappelmeyer ◽  
Matthias Kästner ◽  
...  
Keyword(s):  
Environmental Pollution ◽  
Large Scale ◽  
Pilot Scale ◽  
Wastewater Disposal ◽  
Interdisciplinary Approaches ◽  
Phenolic Wastewater ◽  
Term Monitoring

The large scale of the contamination by the former carbo-chemical industry in Germany requires new and often interdisciplinary approaches for performing an economically sustainable remediation. For example, a highly toxic and dark-colored phenolic wastewater from a lignite pyrolysis factory was filled into a former open-cast pit, forming a large wastewater disposal pond. This caused an extensive environmental pollution, calling for an ecologically and economically acceptable strategy for remediation. Laboratory-scale investigations and pilot-scale tests were carried out. The result was the development of a strategy for an implementation of full-scale enhanced in situ natural attenuation on the basis of separate habitats in a meromictic pond. Long-term monitoring of the chemical and biological dynamics of the pond demonstrates the metamorphosis of a former highly polluted industrial waste deposition into a nature-integrated ecosystem with reduced danger for the environment, and confirmed the strategy for the chosen remediation management.

scholarly journals Analysis of fatty acids of human red cells without lipid extraction

1968 ◽  
Vol 9 (2) ◽  
pp. 285-286
Author(s):  
Gerald B. Phillips ◽  
James T. Dodge ◽  
Carole S. Rockmore
Keyword(s):  
Fatty Acids ◽  
Lipid Extraction ◽  
Red Cells ◽  
Human Red Cells

Effect of n-3 long-chain polyunsaturated fatty acids supplementation in healthy mothers on DHA and EPA profiles in maternal and umbilical blood: a randomized controlled trial

2019 ◽  
Vol 47 (2) ◽  
pp. 200-206
Author(s):  
Dragan Soldo ◽  
Matija Mikulić-Kajić ◽  
Lara Spalldi Barišić ◽  
Nikolina Penava ◽  
Martina Orlović ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Randomized Controlled Trial ◽  
Corn Oil ◽  
Controlled Trial ◽  
Lipid Extraction ◽  
Control Group ◽  
Randomized Controlled ◽  
Umbilical Blood ◽  
Epa And Dha ◽  
The Impact

AbstractBackgroundThe objective of the study was to compare the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) dietary supplementation on their concentration in total lipids (TL) and lipid fractions of maternal and umbilical vein (UV) blood. The specific objective was to analyze the impact of EPA and DHA supplementation on pregnancy outcome and neonatal birth weight.MethodsWomen were randomly single-blinded (randomized controlled trial; ISRCTN36705743) allocated to the group receiving EPA and DHA supplementation (supplemented group) or the group receiving placebo-corn oil (control group) in the time period from January 1st, 2016 until March 1st, 2017. Women in the supplemented group (n=45) took 360 mg EPA and 240 mg DHA daily while controls (n=42) were given a placebo. Maternal and UV bloods were obtained at delivery. After lipid extraction, phospholipids (PL), cholesterol esters (CE), triacylglycerols (TG) and non-esterified fatty acids were separated by thin layer chromatography and analyzed by gas chromatography.ResultsHigher DHA concentrations in TL (37.24±21.87 mg/L), PL (13.14±8.07 mg/L) and triacylglycerols (2.24±2.21 mg/L) were recorded in mothers from the supplemented group when compared to the study group (TL 21.89±14.53 mg/L; P<0.001; PL 9.33±5.70 mg/L; P=0.013; TG 0.56±0.43 mg/L; P<0.001). Higher DHA concentrations in UV samples were found in TL (11.51±7.34 mg/L), PL (5.29±3.31 mg/L) and triacylglycerols (0.62±0.46 mg/L) from the supplemented groups compared with controls (TL 7.37±3.60 mg/L; P=0.002; PL 3.52±2.19 mg/L; P=0.005; TG 0.40±0.46 mg/L; P=0.035). The ratio of AA:DHA was lower in maternal (2.43) and UV serum (4.0) of the supplemented group than in the control group (maternal 3.85 P<0.001; UV 4.91 P<0.001).ConclusionThe study demonstrated the higher ratio of AA/DHA in the control group indicating that pregnant women on the traditional Herzegovina diet need supplementation with DHA and EPA.

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