696 RESULTS OF A MULTI-CENTER EVALUATION OF A NEW RAPID TEST FOR DETECTION OF HCV INFECTION USING WHOLE BLOOD, SERUM, PLASMA AND ORAL FLUID

Abstract Background From 2014 to 2015, the syphilis rate in the United States increased by 19%, reaching its highest rate since 1994. Currently, point-of-care syphilis assays use fingerstick or venipuncture whole blood to identify Treponema pallidum (TP) antibodies by qualitative immunoassay. However, patients and providers prefer oral fluid testing to whole blood testing. In this study, we aimed to determine whether a rapid syphilis test intended for use on whole blood could be used to detect TP antibodies in oral fluid. Methods Oral fluid was collected from 72 participants using the Super•SAL™ Oral Fluid Collection Device (Oasis Diagnostics®, Vancouver, WA). The device uses an absorbent cylindrical pad to collect and filter ~1 mlml of oral fluid. Oral fluid filtrate was tested using the SD Bioline Syphilis 3.0 rapid test (Alere Diagnostics, MA) following manufacturer directions for whole blood. TP particle agglutination (TPPA) and rapid plasma reagin (RPR) results derived from participants’ medical records were used as reference values. We used three different definitions as comparators: 1: TPPA reactive; 2: TPPA and RPR reactive and 3: TPPA reactive and RPR titer >1:4. Those with non-reactive TPPA and RPR results were considered seronegative. We calculated the sensitivity and specificity for definition 1 and sensitivity for definitions 2 and 3. We used the exact binomial method to determine 95% confidence intervals (CI). Results With definitions 1, 2, and 3, respectively, sensitivity was 83.3% (CI: 67.2, 93.6), 86.4% (CI: 65.1, 97.1), and 100% (CI: 71.5, 100). Specificity was 47.2% (CI: 36.5, 75.5). Conclusion The high sensitivity of the SD Bioline Syphilis 3.0 test using oral fluid suggests a strong potential for the development of accurate rapid oral syphilis tests. Sensitivity increased with higher RPR titer. False positive results may be due to the presence of non-venereal treponemal antibodies in oral fluid. Further research and development are needed to optimize specificity. Disclosures All authors: No reported disclosures.

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Correction: Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid

PLoS ONE ◽

10.1371/journal.pone.0232727 ◽

2020 ◽

Vol 15 (4) ◽

pp. e0232727

Author(s):

Maria Carmen Arroyo Sanchez ◽

Beatriz Julieta Celeste ◽

José Angelo Lauletta Lindoso ◽

Mahyumi Fujimori ◽

Roque Pacheco de Almeida ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Blood Serum ◽

Diagnostic Test ◽

Rapid Diagnostic Test ◽

Whole Blood ◽

Oral Fluid

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Assessment of the Accuracy of Whole Blood/Serum Rapid Point-of-Care HIV Three Dot Test for Oral Fluid Specimens

Current HIV Research ◽

10.2174/1570162x14666160321120123 ◽

2016 ◽

Vol 14 (4) ◽

pp. 354-359 ◽

Cited By ~ 1

Author(s):

N. Rakesh ◽

Shakilla Shetty ◽

S. Sujatha ◽

Shivani Sharma ◽

Ankit Saxena

Keyword(s):

Blood Serum ◽

Whole Blood ◽

Oral Fluid ◽

Point Of Care

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Evaluation of a new, rapid test for detecting HCV infection, suitable for use with blood or oral fluid

Journal of Virological Methods ◽

10.1016/j.jviromet.2010.12.009 ◽

2011 ◽

Vol 172 (1-2) ◽

pp. 27-31 ◽

Cited By ~ 64

Author(s):

Stephen R. Lee ◽

Keith W. Kardos ◽

Eugene Schiff ◽

Cheryl A. Berne ◽

Karam Mounzer ◽

...

Keyword(s):

Oral Fluid ◽

Rapid Test ◽

Hcv Infection

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Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid

PLoS ONE ◽

10.1371/journal.pone.0230610 ◽

2020 ◽

Vol 15 (4) ◽

pp. e0230610 ◽

Cited By ~ 2

Author(s):

Maria Carmen Arroyo Sanchez ◽

Beatriz Julieta Celeste ◽

José Angelo Lauletta Lindoso ◽

Mahyumi Fujimori ◽

Roque Pacheco de Almeida ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Blood Serum ◽

Diagnostic Test ◽

Rapid Diagnostic Test ◽

Whole Blood ◽

Oral Fluid

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Antiheparin Activity of Human Serum and Platelet Factor 4

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649105 ◽

1973 ◽

Vol 30 (01) ◽

pp. 093-105 ◽

Cited By ~ 1

Author(s):

C.H.J Sear ◽

L Poller ◽

F.R.C Path

Keyword(s):

Molecular Weight ◽

Ionic Strength ◽

Blood Serum ◽

Whole Blood ◽

Platelet Rich Plasma ◽

Gel Filtration ◽

Normal Serum ◽

Platelet Factor ◽

Mean Values ◽

Antiheparin Activity

SummaryThe antiheparin activity of normal serum has been studied by comparing the antiheparin activities of sera obtained from normal whole blood, platelet-rich plasma and platelet-’free’ plasma with a purified platelet extract during differential isoelectric precipitation and by gel filtration chromatography.The mean values for the activity of PRP-serum and PFP-serum were 106% (S.D. 11) and 10% (S.D. 3) of untreated whole blood respectively. The activity of whole blood serum, PRP serum and whole blood serum plus platelet extract precipitated under identical physical conditions, i.e. pH 7.0, I =0.008, indicating that the activities of the three samples are probably associated with PF4. PF4 precipitated from human platelet extract at pH 4.0, but this is probably due to the difference in the two biochemical environments investigated, i.e. serum and platelet extract.The gel filtration experiments revealed striking similarities between the major antiheparin activities of serum and platelet extract. At physiological pH and ionic strength both activities were associated with high molecular weight material, but at physiological pH and elevated ionic strength both activities behaved as much smaller entities of molecular weight between 25,000 and 30,000 daltons and it seems very likely that both activities are associated with the same molecule, i.e. PF4.

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Prevalence and Risk Factors of Hepatitis C Virus (HCV) in Tehsil Batkhela District Malakand, KPK, Pakistan

International Journal of Contemporary Research and Review ◽

10.15520/ijcrr/2018/9/06/525 ◽

2018 ◽

Vol 9 (06) ◽

pp. 20251-20256

Author(s):

Mudassir Khan ◽

Shahrukh Khan ◽

Shohra Haider ◽

Fazal Jalil ◽

Muhsin Jamal ◽

...

Keyword(s):

Risk Factors ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Skin Color ◽

Significant Risk ◽

Rapid Test ◽

Prevalence Ratio ◽

Hcv Infection ◽

Hcv Rna ◽

Common Symptom

Background: Prevalence of Hepatitis C viral infection and its major risk factors has been found out in population of Batkhela, Khyber Pakhtunkhwa, Pakistan by taking number of volunteers from the interested area. HCV prevalence has not been researched in recent time here in this area, so that’s why we contributed. Materials and Methods: Ab rapid test cassette serum/plasma (USA) kit has been used for the mentioned purpose following by ELISA and finally PCR to find out active infection of virus. ICT positive individuals were reconfirmed by ELISA and then ELISA positive samples were carefully investigated by RT-PCR for Hepatitis C Virus. Results: The study population was of 770 volunteers belonging to the mentioned area of research, 453 males and 317 females. The overall prevalence was found to be 5.32% of HCV in Batkhela. This prevalence ratio was 3.12% in males and 2.20 % in females. 3rd generation ELISA was used to refine ICT positive samples which showed that 37 of the ICT positive samples had antibodies detected by ELISA. To find out active HCV infection, ELISA positive samples were refined by real time PCR which showed 2.98% of prevalence of active HCV infection in Batkhela based on HCV RNA in their blood. Principle Conclusion: Overall prevalence was found 5.32%, contaminated reused syringes and blades at Barbour’s shop, blood transfusion, surgical operations and unhygienic food in stalls etc were found significant risk factors for acquiring HCV infection. Body weakness and pale yellow skin color was common symptom in HCV positive volunteers. Safe sexual activities, blood screening before donation and sterilizing surgical equipment’s can protect us from Hepatitis C Virus.

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DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v21i3.738 ◽

2016 ◽

Vol 21 (3) ◽

pp. 261

Author(s):

Ranti Permatasari ◽

Aryati Aryati ◽

Budi Arifah

Keyword(s):

Hepatitis C ◽

Predictive Value ◽

Core Protein ◽

Rapid Test ◽

Antibody Test ◽

Diagnostic Value ◽

Hcv Infection ◽

Antibody Detection ◽

Hcv Rna ◽

Hcv Antibody

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to the recipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid test that could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multiple antibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. The samples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infection test using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatography method showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictive value of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody pattern was four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies. Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method has a good diagnostic value.

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