Rapid Diagnosis of Cervical Tuberculous Lymphadenitis by Application of DNA Probe

The purpose of this study was to evaluate the accuracy of the GeneXpert M. tuberculosis (MTB)/ rifampicin (RIF) test for the detection of MTB in lymph node aspirated samples. This study was conducted in the Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Bangladesh. This study was done during the period from July 2013 to May 2015. A total of 317 clinically suspected tuberculous lymphadenitis patients without malignancy were included in the study. The culture test and GeneXpert test were used for detection of MTB in lymph node aspirated material. Among the 317 samples tested, the GeneXpert detected the DNA of MTB in 167 samples (52.7%), whereas culture test was positive in 74 (23.3%) specimens. GeneXpert also detected 8 RIF resistance cases. GeneXpert sensitivity and specificity results were assessed according to culture results. The sensitivity and specificity of the GeneXpert assay was 95.9% and 60.5%, respectively. The implementation of the GeneXpert MTB/RIF assay may dramatically improve the rapid diagnosis of lymph node TB. The GeneXpert MTB/RIF may replace usual conventional method like culture test for detection of MTB. Mediscope Vol. 6, No. 1: Jan 2019, Page 19-23

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EBUS-TBNA in the rapid microbiological diagnosis of drug-resistant mediastinal tuberculous lymphadenopathy

ERJ Open Research ◽

10.1183/23120541.00008-2019 ◽

2019 ◽

Vol 5 (4) ◽

pp. 00008-2019

Author(s):

Prashant N. Chhajed ◽

Preyas J. Vaidya ◽

Neha P. Mandovra ◽

Vinod B. Chavhan ◽

Tejashree T. Lele ◽

...

Keyword(s):

Needle Aspiration ◽

Drug Susceptibility Testing ◽

Rapid Diagnosis ◽

Endobronchial Ultrasound ◽

Rifampicin Resistance ◽

Tuberculous Lymphadenitis ◽

Drug Resistant ◽

Transbronchial Needle Aspiration ◽

Mdr Tb ◽

Culture Positive

This study aimed to examine the use of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in the rapid diagnosis of mediastinal tuberculous lymphadenitis and drug-resistant mediastinal tuberculous lymphadenitis.A diagnosis of TB was confirmed by a positive Xpert MTB/RIF test or Mycobacterium tuberculosis culture. Rifampicin-resistant TB (RR-TB) or multidrug-resistant TB (MDR-TB) was diagnosed upon the detection of rifampicin resistance by Xpert MTB/RIF or resistance to rifampicin and isoniazid by phenotypic drug susceptibility testing (DST).Xpert MTB/RIF was positive in 43 of 56 patients (77%) and TB culture was positive in 31 of 56 patients (55%). Of these 56 patients, 25 (45%) were Xpert MTB/RIF positive and TB culture negative, 13 (23%) were Xpert MTB/RIF negative and TB culture positive, and 18 (32%) were Xpert MTB/RIF positive and TB culture positive. 11 patients (20%) had drug-resistant TB: seven with RR/MDR-TB, one with pre-extensively drug-resistant (XDR) TB, two with XDR-TB and one with isoniazid mono-resistance.An Xpert MTB/RIF assay carried out on EBUS-TBNA specimens provides rapid diagnosis of TB. Xpert MTB/RIF testing appears to have additional and more rapid sensitivity compared with culture alone. Culture-based DST provides an additional exclusive yield and the full resistance profile in addition to or instead of rifampicin resistance.

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Clinical performance of Xpert MTB/RIF on contrast-enhanced ultrasound-guided core biopsy specimens for rapid diagnosis of superficial tuberculous lymphadenitis in high TB burden settings

Infection ◽

10.1007/s15010-021-01578-w ◽

2021 ◽

Author(s):

Wenwen Sun ◽

Jin Gu ◽

Ke Bi ◽

Yi Zhang ◽

Meng-jun Shen ◽

...

Keyword(s):

Core Biopsy ◽

Clinical Performance ◽

Rapid Diagnosis ◽

Tuberculous Lymphadenitis ◽

Contrast Enhanced Ultrasound ◽

Ultrasound Guided ◽

Biopsy Specimens ◽

Contrast Enhanced

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USE OF CBNAAT X-PERT MTB FOR RAPID DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS FROM FINE NEEDLE ASPIRATION CYTOLOGY SPECIMENS AT A TERTIARY CARE HOSPITAL

10.36106/ijsr/0501372 ◽

2019 ◽

pp. 45-47

Author(s):

Khiste J.A ◽

Deshpande N. M ◽

Pandit G.A ◽

Londhe S.S

Keyword(s):

Fluorescence Microscopy ◽

Fine Needle Aspiration ◽

Fine Needle Aspiration Cytology ◽

Needle Aspiration ◽

Rapid Diagnosis ◽

Tuberculous Lymphadenitis ◽

Care Hospital ◽

Aspiration Cytology ◽

Fine Needle ◽

Needle Aspiration Cytology

Background: Diagnosis of extrapulmonary tuberculosis is difficult. Tuberculous lymphadenitis is one of the most common extrapulmonary manifestation of tuberculosis.1 Cervical group of lymph nodes are commonly involved.2 Fine needle aspiration cytology can be used easily, safely for specimen collection of these cases. The cytology samples can be used for CBNAAT X-pert assay for rapid diagnosis of tuberculous lymphadenitis along with fluoroscence microscopy. The aim of the present study was to evaluate proportion of tuberculous lymphadenitis from FNAC specimens of all lymphadenopathy cases, to study diagnostic utility of CBNAAT X-pert MTB for diagnosis of tuberculous lymphadenopathy in patients with lymphadenitis and to correlate cytological findings with CBNAATand fluorescence microscopy results. Methods:The present study was included 45 cases of lymphadenopathy. Detailed history and physical examination was done followed by FNAC . Multiple smears were prepared from each aspirate for routine cytological examination and for evaluation by fluroscence microscopy. The cytology findings were correlated with CBNAATand fluoroscence microscopy results. Results:In the present study female preponderence was noted. Maximum number of cases were in 3rd decade. Tuberculous lymphadenitis was the most common cytological diagnosis found in 51.1 % cases, of which 33.3% were positive on CBNAAT and 20% cases were positive by fluorescence microscopy. Conclusion: The most common cause of cervical lymphadenopathy is tuberculosis. The present study supports combined use of FNAC and CBNAATfor early diagnosis of tuberculosis.

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