scholarly journals Diagnosis of lymph node tuberculosis using the GeneXpert MTB/RIF in Bangladesh

Mediscope ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 19-23
Author(s):  
TE Nur ◽  
AU Hosna ◽  
N Rayhan ◽  
N Nazneen
Keyword(s):  
Lymph Node ◽  
Sensitivity And Specificity ◽  
Conventional Method ◽  
Rapid Diagnosis ◽  
Tuberculous Lymphadenitis ◽  
Culture Test ◽  
Lymph Node Tuberculosis ◽  
Medical University

The purpose of this study was to evaluate the accuracy of the GeneXpert M. tuberculosis (MTB)/ rifampicin (RIF) test for the detection of MTB in lymph node aspirated samples. This study was conducted in the Department of Pathology, Bangabandhu Sheikh Mujib Medical University, Bangladesh. This study was done during the period from July 2013 to May 2015. A total of 317 clinically suspected tuberculous lymphadenitis patients without malignancy were included in the study. The culture test and GeneXpert test were used for detection of MTB in lymph node aspirated material. Among the 317 samples tested, the GeneXpert detected the DNA of MTB in 167 samples (52.7%), whereas culture test was positive in 74 (23.3%) specimens. GeneXpert also detected 8 RIF resistance cases. GeneXpert sensitivity and specificity results were assessed according to culture results. The sensitivity and specificity of the GeneXpert assay was 95.9% and 60.5%, respectively. The implementation of the GeneXpert MTB/RIF assay may dramatically improve the rapid diagnosis of lymph node TB. The GeneXpert MTB/RIF may replace usual conventional method like culture test for detection of MTB. Mediscope Vol. 6, No. 1: Jan 2019, Page 19-23

scholarly journals Comparison of the Microwave-Heated Ziehl-Neelsen Stain and Conventional Ziehl-Neelsen Method in the Detection of Acid-Fast Bacilli in Lymph Node Biopsies

2019 ◽  
Vol 7 (6) ◽  
pp. 903-907
Author(s):  
Gehan Mohammed Ahmed ◽  
Ali Seed Ahmed Mohammed ◽  
Albadawi Abdulbagi Taha ◽  
Ahmad Almatroudi ◽  
Khaled S. Allemailem ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sensitivity And Specificity ◽  
Demographic Data ◽  
Chronic Inflammatory Disease ◽  
Military Hospital ◽  
Routine Practice ◽  
Predictive Values ◽  
Acid Fast Bacilli ◽  
Lymph Node Tuberculosis ◽  
Low Sensitivity

BACKGROUND: Tuberculosis is a chronic inflammatory disease with lymphadenopathy being the most common extra-pulmonary manifestation. The conventional Ziehl–Neelsen method plays an essential role in the diagnosis of tuberculosis; however, it has a low sensitivity in detecting acid-fast bacilli. AIM: The present study emphasises the role of the microwave-heated method (modified Ziehl–Neelsen) over conventional Ziehl-Neelsen stain and to set at the best condition for irradiation. MATERIAL AND METHODS: The study included 90 patients with clinically suspected tuberculous lymphadenopathy who were referred to the Department of Pathology at Omdurman Military Hospital, Sudan. Demographic data such as age, sex, and site of swelling were documented for each patient. Specimens were stained with conventional Ziehl-Neelsen, fluoresce and the modified methods. RESULTS: Patient’s age ranged from 20 to 70 year. Of the total 90 cases with clinically suspected tuberculous lymphadenopathy, 18 cases were positive for AFB in conventional Ziehl-Neelsen method giving a sensitivity of 13.3%, while in microwave-heated method 82 cases of TB were detected positive for AFB yielded sensitivity and specificity of 97.6% and 85.7%, respectively, and positive and negative predictive values of 98.8% and 75.0% respectively compared to fluorescence methods. CONCLUSION: In the present study, the microwave-heated Ziehl-Neelsen method, was found to have sensitivity and specificity of 97.6% and 85.7%, respectively which matches the fluorescence technique. It has specificity in detecting lymph node tuberculosis that makes it superior over all other modified methods. However, the availability and cost-effectiveness might limit the use of fluorescence in routine practice. Furthermore, the study set the best staining temperature is provided at power 1 level (60 w) for 1.5 minutes.

scholarly journals Case report: association between lymph node tuberculosis and breast cancer

Mastology ◽  
2020 ◽  
Vol 30 (Suppl 1) ◽  
Author(s):  
Marina Fontes Medeiros ◽  
Gustavo Lanza de Melo ◽  
Thássia Mariz de Melo ◽  
Rachel Saraiva Teatini Selim de Sales ◽  
Janaina Cotta Rodrigues Ferreira
Keyword(s):  
Breast Cancer ◽  
Case Report ◽  
Lymph Node ◽  
Pulmonary Nodules ◽  
Southeastern Brazil ◽  
Tuberculous Lymphadenitis ◽  
Axillary Lymph ◽  
Safety Margins ◽  
Lymph Node Tuberculosis ◽  
The Right

Introduction: Lymph node tuberculosis is the most common extra-lung presentation of tuberculosis, responsible for 43% of peripheral lymphadenopathies in developing countries. The coexistence between lymph node tuberculosis and breast cancer is rare, ranging from 0.1% to 4.9%.Objective: To present a case of axillary lymph node tuberculosis due to its rare association with breast cancer. Methods: We have investigated the case of a 48-year-old woman from Congonhas, Minas Gerais, Southeastern Brazil, who presented a palpable nodule in the junction of the right upper quadrants with two years of progression, category 4B of the Breast Imaging Reporting and Data System (BI-RADS) on mammography and ultrasound, with core needle biopsy compatible with benignancy. No axillary lymphadenopathy was identified. Case report: The patient underwent resection of the right breast nodule with safety margins due to disagreement between biopsy and imaging tests. Anatomopathological examination was consistent with luminal B invasive ductal carcinoma, measuring 1.6 cm. The patient was submitted to sentinel lymph node biopsy using patent blue in the right axilla. Anatomopathological analysis revealed tuberculous lymphadenitis. Chest computed tomography showed pulmonary nodules. The patient received adjuvant radiotherapy and tamoxifen, as well as antituberculous antibiotics, with regression of pulmonary nodules. The final staging was pT1cN0M0- IA. Discussion: Most cases of coexistence between these diseases involve tuberculous lymphadenitis with or without neoplastic lymph node involvement. Some reports indicate that the involvement by tuberculosis does not prevent neoplastic proliferation. Before starting chemotherapy, tuberculosis must be treated to avoid the immunosuppressive effect that can cause a spread of tuberculosis. Conclusion: Despite the rare coexistence of these diseases, we should not rule out this possibility, especially in endemic tuberculosis areas. Also, an accurate diagnosis prevents incorrect staging and can spare the patient from a more aggressive treatment.

scholarly journals Diagnostic Accuracy of the Xpert MTB/RIF Assay for Lymph Node Tuberculosis: A Systematic Review and Meta-Analysis

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Guocan Yu ◽  
Fangming Zhong ◽  
Bo Ye ◽  
Xudong Xu ◽  
Da Chen ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sensitivity And Specificity ◽  
Meta Analysis ◽  
Needle Aspiration ◽  
Diagnostic Efficiency ◽  
Tissue Samples ◽  
Significant Difference ◽  
Lymph Node Tuberculosis ◽  
Meta Analyses ◽  
Composite Reference Standard

Objectives. To evaluate the performance of Xpert MTB/RIF for lymph node tuberculosis (LNTB). Methods. We searched databases for published reports. We reviewed the studies and identified the performance of Xpert MTB/RIF with respect to a composite reference standard (CRS) and culture. We used a bivariate random-effects model to perform meta-analyses and used metaregression to analyze sources of heterogeneity. Results. 15 independent studies compared Xpert MTB/RIF with CRS while 21 comparing it with culture were included. The pooled sensitivity and specificity of Xpert MTB/RIF were 79% and 98% compared to that of CRS, respectively, and 84% and 91% compared to that of culture, respectively. The pooled sensitivity and specificity using fine needle aspiration (FNA) samples versus CRS were 80% and 96%, whereas those against culture were 90% and 89%, respectively. The percentages while working with tissue samples versus CRS were 76% and 100%, respectively, whereas those against culture were 76% and 92%, respectively. There was no significant difference in diagnostic efficiency among the types of specimen. Conclusions. Xpert MTB/RIF demonstrates good diagnostic efficiency for LNTB and is not related to the type of specimen, obtained via different routes.

scholarly journals Histopathological Features of Lymph Nodes of Tuberculous Lymphadenitis Patients: Experience of 50 cases in Bangladesh

2017 ◽  
Vol 3 (2) ◽  
pp. 40-42
Author(s):  
Mohammad Mahmudul Huda ◽  
Mohammad Taufiq ◽  
Md Abdullah Yusuf ◽  
Mohammad Rezwanur Rahman ◽  
Ferdousy Begum ◽  
...  
Keyword(s):  
Lymph Node ◽  
Relevant Information ◽  
Cross Sectional Study ◽  
Tuberculous Lymphadenitis ◽  
Cross Sectional ◽  
Extra Pulmonary Tuberculosis ◽  
Histopathological Features ◽  
Fresh Specimen ◽  
Medical University ◽  
Patients Experience

Background: Tuberculous lymphadenitis is common manifestation of extra-pulmonary tuberculosis in Bangladesh. Objective: The purpose of the present study was to see the histopathological features of tuberculous lymphadenitis patients. Methodology: This cross sectional study was done at the Department of Pathology at Banghabandhu Sheikh Mujib Medical University (BSMMU), Dhaka from January 2009 to March 2011 for a period of nearly two years. All the patients irrespective of age and sex with the clinical features suggestive of tuberculous lymphadenitis and later on proved to be the same histologically were selected for the study purposively. Relevant information was recorded in a prescribed data sheet and histomorphological findings were recorded accordingly. In cases where fresh specimen was available, caseous portion of lymph node was sent for culture. FiteFaraco staining was also done on lymph node sections in all cases. Result: A total number of 50 tuberculous lymphadenitis patients were recruited for this study of which well formed granuloma was detected in 34(68.0%) cases followed by ill defined and combination of well and ill defined which were 8(16.0%) cases in each. Among 34 well defined cases coalescing and discrete were found in 6(17.6%) cases in each. However, both types were found in 22(64.7%) cases.Conclusion: In conclusion well formed granuloma is most commonly found in tuberculous lymphadenitis with combined variety of coalescing and discrete histopathologic featuresBangladesh Journal of Infectious Diseases 2016;3(2):40-42

Use of Ligase Chain Reaction for the Rapid Diagnosis of Lymph Node Tuberculosis

2004 ◽  
Vol 36 (10) ◽  
pp. 724-726 ◽  
Author(s):  
E. León Muiños ◽  
M. L. Pérez Del Molino ◽  
F. L. Lado Lado ◽  
M. Pazo Nuñez ◽  
F. Pardo
Keyword(s):  
Lymph Node ◽  
Rapid Diagnosis ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Lymph Node Tuberculosis

scholarly journals Disseminated lymph node tuberculosis after splenectomy: an unusual case report in an adolescent

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Moxin Cheng ◽  
Yu Chen
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Excisional Biopsy ◽  
Needle Aspiration ◽  
Nucleic Acid Amplification ◽  
Axillary Lymph Nodes ◽  
Tuberculous Lymphadenitis ◽  
Axillary Lymph ◽  
Increased Risk ◽  
Lymph Node Tuberculosis

Abstract Background Splenectomized patients are at an increased risk for overwhelming post-splenectomy infections typically with encapsulated bacteria. The clinical association between splenectomy and lymph-node tuberculosis is unclear. Case presentation We describe a rare case of disseminated tuberculous lymphadenitis in an 18-year-old woman with history of splenectomy because of hereditary sherocytosis. She was admitted with enlargement of bilateral-cervical and left-axillary lymph nodes and fever. A diagnosis of probable tuberculosis was made based on the findings of fine-needle aspiration. Histology showed granulomas and extensive caseous necrosis, with the site of puncture located at an enlarged lymph node on the right side. The diagnosis was confirmed via nucleic-acid amplification tests following excisional biopsy of the left axillary lymph node. Disseminated tuberculous lymphadenitis was localized in the bilateral neck, right lung hilum, left sub-axillary region, and mediastinum, as detected from contrast-enhanced computed tomography of the neck. Conclusions Mycobacterium tuberculosis infection should be considered in children and adolescents with extensive enlargement of lymph nodes after splenectomy.

scholarly journals Paradoxical reactions during Antituberculosis therapy - A single-center prospective analysis

2015 ◽  
Vol 2 (2) ◽  
pp. 32-35
Author(s):  
Sanae Hammi ◽  
Naima Zimed ◽  
Khalid Bouti ◽  
Jamal Eddine Bourkadi
Keyword(s):  
Lymph Node ◽  
Infectious Diseases ◽  
Clinical Microbiology ◽  
Tuberculous Lymphadenitis ◽  
Paradoxical Reaction ◽  
Paradoxical Response ◽  
Antituberculosis Therapy ◽  
Tuberculous Abscess ◽  
Lymph Node Tuberculosis ◽  
Hiv Negative

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A Case of Erythema Nodosum with Lymph Node Tuberculosis and Colon Cancer

2015 ◽  
Vol 77 (1) ◽  
pp. 10-13
Author(s):  
Masayoshi NAKAO ◽  
Satoshi TAKEUCHI ◽  
Kazuhiro TAKAHASHI ◽  
Hiromaro KIRYU ◽  
Hiroshi TERAO ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Lymph Node ◽  
Erythema Nodosum ◽  
Lymph Node Tuberculosis

scholarly journals 1641. A Comparative Analysis Of Lymph Node Tuberculosis Between Children And Adults

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S810-S810
Author(s):  
Fatma Hammami ◽  
Makram Koubaa ◽  
Amal Chakroun ◽  
Khaoula Rekik ◽  
Fatma Smaoui ◽  
...  
Keyword(s):  
Lymph Node ◽  
Family History ◽  
Extrapulmonary Tuberculosis ◽  
Diagnostic Delay ◽  
Disease Evolution ◽  
Evolutionary Features ◽  
Significant Difference ◽  
History Of ◽  
Adults And Children ◽  
Lymph Node Tuberculosis

Abstract Background Lymph node tuberculosis (LNTB) represents the most common site of extrapulmonary tuberculosis. Among children, due to non-specific clinical features, the diagnosis is often delayed. We aimed to compare the clinical, therapeutic and evolutionary features of LNTB between adults and children. Methods We conducted a retrospective study including patients hospitalized for LNTB in the infectious diseases and pediatric department between 1993 and 2018. Children aged ≤18 years were included. Results Overall, we encountered 231 cases of LNTB. There were 40 children (17.3%) with a mean age of 11±4 years and 191 adults (82.7%) with a mean age of 42±16 years. As to gender, females were more affected (adults: 67% vs children: 70%), with no significant difference (p >0.05). A family history of tuberculosis was significantly more frequent among children (20% vs 6.3%; p=0.01). Raw milk consumption (38.2% vs 30%; p >0.05) and close contact with animals (29.8% vs 35%; p >0.05) were noted among both adults and children. Fever (53.4% vs 32.5%; p=0.01), night sweats (35.8% vs 10%; p=0.001), loss of appetite (38.2% vs 17.5%; p=0.01) and weight loss (35.1% vs 15%; p=0.01) were significantly more frequent among adults. Tuberculin skin test was positive in 75.8% of the cases among adults and in 86.2% of the cases among children (p >0.05). Multifocal tuberculosis was significantly more frequent among adults (23.8% vs 5.7%; p=0.01). Antitubercular therapy was prescribed for a mean duration of 10±4 months among adults and for 9±3 months among children, with no significant difference (p >0.05). Side effects of antitubercular drugs were more frequent among adults (33% vs 10.3%), with a significant difference (p=0.004). Comparison of the disease evolution showed no significant difference between adults and children, regarding recovery (94.8% vs 90%), relapse (5.2% vs 5%) and death (0.5% vs 2.5%). Conclusion The clinical presentation of LNTB among children was less common and misleading. A family history of tuberculosis and a high index of suspicion might shorten the diagnostic delay. Disclosures All Authors: No reported disclosures

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