Construction and characterization of rhesus monkey rotavirus (MMU18006)- or bovine rotavirus (UK)-based serotype G5, G8, G9 or G10 single VP7 gene substitution reassortant candidate vaccines

Vaccine ◽  
2003 ◽  
Vol 21 (21-22) ◽  
pp. 3003-3010 ◽  
Author(s):  
Yasutaka Hoshino ◽  
Ronald W Jones ◽  
Jerri Ross ◽  
Albert Z Kapikian
Keyword(s):  
Rhesus Monkey ◽  
Gene Substitution ◽  
Bovine Rotavirus ◽  
Vp7 Gene

A bovine rotavirus serotype 1: Serologic characterization of the virus and nucleotide sequence determination of the structural glycoprotein VP7 gene

Virology ◽  
1992 ◽  
Vol 189 (2) ◽  
pp. 833-837 ◽  
Author(s):  
Jorge Blackhall ◽  
Rodolfo Bellinzoni ◽  
Nora Mattion ◽  
Mary K. Estes ◽  
JoséL. La Torre ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Sequence Determination ◽  
Bovine Rotavirus ◽  
Serotype 1 ◽  
Nucleotide Sequence Determination ◽  
Vp7 Gene

Characterization of a cDNA for Rhesus Monkey Protein C Inhibitor – Evidence for N-Terminal Involvement in Heparin Stimulation

1995 ◽  
Vol 74 (04) ◽  
pp. 1079-1087 ◽  
Author(s):  
Klaus-P Radtke ◽  
José A Fernández ◽  
Bruno O Villoutreix ◽  
Judith S Greengard ◽  
John H Griffin
Keyword(s):  
Rhesus Monkey ◽  
Protein C ◽  
Activated Protein C ◽  
Pcr Amplification ◽  
Amino Acid Sequences ◽  
Heparin Binding ◽  
Reactive Center ◽  
Protein C Inhibitor ◽  
Polymerase Chain

SummarycDNAs for protein C inhibitor (PCI) were cloned from human and rhesus monkey 1 liver RNAs by reverse transcription and polymerase chain reaction (PCR) amplification. Sequencing showed that rhesus monkey and human PCI cDNAs were 93% identical. Predicted amino acid sequences differed at 26 of 387 residues. Pour of these differences (T352M, N359S, R362K, L3631) were in the reactive center loop that is important for inhibitory specificity, and two were in the N-terminal helix (M8T, E13K) that is implicated in glycosaminoglycan binding. PCI in human or rhesus monkey plasma showed comparable inhibitory activity towards human activated protein C in the presence of 10 U/ml heparin. However, maximal acceleration of the inhibition of activated protein C required 5-fold lower heparin concentration for rhesus monkey than for human plasma, consistent with the interpretation that the additional positive charge (E13K) in a putative-heparin binding region increased the affinity for heparin.

Cloning and characterization of rhesus monkey MCH-R1 and MCH-R2

Peptides ◽  
2002 ◽  
Vol 23 (8) ◽  
pp. 1401-1408 ◽  
Author(s):  
Steven Fried ◽  
Kim O’Neill ◽  
Brian E Hawes
Keyword(s):  
Rhesus Monkey

scholarly journals Two out of the 11 genes of an unusual human G6P[6] rotavirus isolate are of bovine origin

2008 ◽  
Vol 89 (10) ◽  
pp. 2630-2635 ◽  
Author(s):  
Jelle Matthijnssens ◽  
Mustafizur Rahman ◽  
Marc Van Ranst
Keyword(s):  
Complete Genome ◽  
Bovine Rotavirus ◽  
Rotavirus Vaccines ◽  
Human Rotavirus ◽  
New Gene ◽  
Molecular Origin ◽  
Encoding Gene ◽  
Vp7 Gene

In 2003, we described the first human G6P[6] rotavirus strain (B1711). To investigate further the molecular origin of this strain and to determine the possible reassortments leading to this new gene constellation, the complete genome of strain B1711 was sequenced. SimPlot analyses were conducted to compare strain B1711 with other known rotavirus gene segments, and phylogenetic dendrograms were constructed to analyse the origin of the eleven genome segments of strain B1711. Our analysis indicated that strain B1711 acquired its VP1-, VP2-, VP4-, VP6- and NSP1–5-encoding gene segments from human DS-1-like P[6] rotavirus strains, and its VP3 and VP7 gene segments from a bovine rotavirus strain through reassortment. The introduction of animal–human reassortant strains, which might arise in either of the hosts, into the human rotavirus population is an important mechanism for the generation of rotavirus diversity, and might be a challenge for the current rotavirus vaccines and vaccines under development.

scholarly journals The Spike Protein VP4 Defines the Endocytic Pathway Used by Rotavirus To Enter MA104 Cells

2012 ◽  
Vol 87 (3) ◽  
pp. 1658-1663 ◽  
Author(s):  
Marco A. Díaz-Salinas ◽  
Pedro Romero ◽  
Rafaela Espinosa ◽  
Yasutaka Hoshino ◽  
Susana López ◽  
...  
Keyword(s):  
Surface Protein ◽  
Endocytic Pathway ◽  
Spike Protein ◽  
Single Amino Acid ◽  
Cellular Receptors ◽  
Hypertonic Medium ◽  
Bovine Rotavirus ◽  
Single Amino Acid Change ◽  
Interfering Rna

ABSTRACTRotaviruses are internalized into MA104 cells by endocytosis, with different endocytic pathways used depending on the virus strain. The bovine rotavirus UK strain enters cells through a clathrin-mediated endocytic process, while the simian rhesus rotavirus (RRV) strain uses a poorly defined endocytic pathway that is clathrin and caveolin independent. The viral surface protein VP7 and the spike protein VP4 interact with cellular receptors during cell binding and penetration. To determine the viral protein that defines the mechanism of internalization, we used a panel of UK × RRV reassortant viruses having different combinations of the viral structural proteins. Characterization of the infectivities of these reassortants in MA104 cells either transfected with a small interfering RNA (siRNA) against the heavy chain of clathrin or incubated with hypertonic medium that destabilizes the clathrin coat clearly showed that VP4 determines the pathway of virus entry. Of interest, the characterization of Nar3, a sialic acid-independent variant of RRV, showed that a single amino acid change in VP4 shifts the route of entry from being clathrin dependent to clathrin independent. Furthermore, characterizations of several additional rotavirus strains that differ in their use of cellular receptors showed that all entered cells by clathrin-mediated endocytosis, suggesting that diverse VP4-cell surface interactions can lead to rotavirus cell entry through this endocytic pathway.

Molecular characterization of rhesus monkey Mhc-DR region: full length cDRA/cDRB analysis

2003 ◽  
Vol 64 (10) ◽  
pp. S164
Author(s):  
Nanine de Groot ◽  
Gaby G.M. Doxiadis ◽  
Natasja G. de Groot ◽  
Nel Otting ◽  
Corrine M.C. Heijmans ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Molecular Characterization ◽  
Full Length

scholarly journals Molecular characterization of G and P-types bovine rotavirus strains from Goiás, Brazil: high frequency of mixed P-type infections

2010 ◽  
Vol 105 (8) ◽  
pp. 1040-1043 ◽  
Author(s):  
Thabata Alessandra Ramos Caruzo ◽  
Willia Marta Elsner Diederichsen de Brito ◽  
Veridiana Munford ◽  
Maria Lúcia Rácz
Keyword(s):  
Molecular Characterization ◽  
High Frequency ◽  
Bovine Rotavirus ◽  
P Type

scholarly journals Detection and Differentiation of Bovine Group A Rotavirus Serotypes using Polymerase Chain Reaction-Generated Probes to the VP7 Gene

1992 ◽  
Vol 4 (2) ◽  
pp. 148-158 ◽  
Author(s):  
Anil V. Parwani ◽  
Blair I. Rosen ◽  
Jorge Flores ◽  
Malcolm A. McCrae ◽  
Mario Gorziglia ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Northern Blot Hybridization ◽  
Chain Reaction ◽  
Diarrhea Virus ◽  
Bovine Rotavirus ◽  
Group A ◽  
Polymerase Chain ◽  
Vp7 Gene ◽  
Hybridization Assays

Dot and Northern blot hybridization assays were developed to detect and differentiate group A bovine rotavirus serotypes using radiolabeled serotype 6 (Nebraska calf diarrhea virus [NCDV] and United Kingdom [UK] strains) or serotype 10 (Cracker [Cr] strain) VP7 gene probes. Partial length VP7-specific cDNA encompassing areas of major sequence diversity were generated by the polymerase chain reaction (PCR) using either cloned VP7 genes (NCDV and UK strains) or reverse transcribed mRNA (Cr strain) as templates. Radiolabeled probes prepared from the PCR-generated cDNA were tested at various stringency conditions to optimize the hybridization assays. At high stringency conditions (52 C, 50% formamide, 5 x standard saline citrate), the NCDV, UK, and Cr probes serotypically differentiated bovine rotavirus isolates in RNA samples prepared from cell culture-propagated viruses or in fecal specimens from infected gnotobiotic calves. The sensitivity and specificity of NCDV and Cr VP7 probes were characterized in dot blot hybridization assays, and the probes were estimated to detect at least 1 ng of viral RNA. The serotyping results obtained using VP7 probes were similar to those obtained using serologic assays. Further development of these assays may provide a useful means for the rapid detection and differentiation of bovine rotavirus serotypes in fecal samples from calves in the field.

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