Site-specific regulation of corticosteroid and serotonin receptor subtype gene expression in the rat hippocampus following 3,4-methylenedioxymethamphetamine: role of corticosterone and serotonin

AbstractThe closely related acetyltransferases CBP and p300 are key regulators of gene expression in metazoans. CBP/p300 acetylate several specific lysine residues within nucleosomes, including histone H3 lysine 27 (H3K27), a hallmark of active enhancers and promoters. However, it has remained largely unclear how specificity of CBP/p300 towards H3K27 is achieved. Here we show that the TAZ2 domain of CBP is required for efficient acetylation of H3K27, while curbing activity towards other lysine residues within nucleosomes. We find that TAZ2 is a sequence-independent DNA binding module, promoting interaction between CBP and nucleosomes, thereby enhancing enzymatic activity and regulating substrate specificity of CBP. TAZ2 is further required to stabilize CBP binding to chromatin in mouse embryonic stem cells, facilitating specificity towards H3K27 and modulating gene expression. These findings reveal a crucial role of TAZ2 in regulating H3K27ac, while highlighting the importance of correct site-specific acetylation for proper regulation of gene expression.

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Role of the serotonin receptor subtype 5-HT1D on basal and stimulated growth hormone secretion

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.80.6.1973 ◽

1995 ◽

Vol 80 (6) ◽

pp. 1973-1977 ◽

Cited By ~ 12

Author(s):

A. Mota

Keyword(s):

Growth Hormone ◽

Serotonin Receptor ◽

Hormone Secretion ◽

Growth Hormone Secretion ◽

Receptor Subtype

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Role of the serotonin receptor subtype 5-HT1D on basal and stimulated growth hormone secretion.

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.80.6.7775648 ◽

1995 ◽

Vol 80 (6) ◽

pp. 1973-1977

Author(s):

A Mota ◽

A Bento ◽

A Peñalva ◽

M Pombo ◽

C Dieguez

Keyword(s):

Growth Hormone ◽

Serotonin Receptor ◽

Hormone Secretion ◽

Growth Hormone Secretion ◽

Receptor Subtype

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Role of the GATA-1/FOG-1/NuRD Pathway in the Expression of Human β-Like Globin Genes

Molecular and Cellular Biology ◽

10.1128/mcb.00001-10 ◽

2010 ◽

Vol 30 (14) ◽

pp. 3460-3470 ◽

Cited By ~ 30

Author(s):

Annarita Miccio ◽

Gerd A. Blobel

Keyword(s):

Gene Expression ◽

Molecular Mechanisms ◽

Globin Gene ◽

Globin Genes ◽

Nurd Complex ◽

Adult Type ◽

Specific Regulation ◽

Globin Gene Expression

ABSTRACT The human β-globin genes are expressed in a developmentally controlled fashion. Studies on the molecular mechanisms underlying the stage-specific regulation of globin genes have been fueled by the clinical benefit of elevated fetal γ-globin expression in patients with sickle cell anemia and thalassemia. Recent reports suggested a role of the hematopoietic transcription factor GATA-1, its cofactor FOG-1, and the associated chromatin remodeling complex NuRD in the developmental silencing of HBG1 and HBG2 gene expression. To examine whether FOG-1 via NuRD controls HBG1 and HBG2 silencing in vivo, we created mice in which the FOG-1/NuRD complex is disrupted (A. Miccio et al., EMBO J. 29:442-456, 2010) and crossed these with animals carrying the entire human β-globin gene locus as a transgene. We found that the FOG-1/NuRD interaction is dispensable for the silencing of human HBG1 and HBG2 expression. In addition, mutant animals displayed normal silencing of the endogenous embryonic globin genes. In contrast, a significant reduction of adult-type human and murine globin gene expression was found in adult bone marrows of mutant animals. These results suggest that, unexpectedly, NuRD is required for FOG-1-dependent activation of adult-type globin gene expression but is dispensable for human γ-globin silencing in vivo.

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Reduced SCD1 activity alters markers of fatty acid reesterification, glyceroneogenesis, and lipolysis in murine white adipose tissue and 3T3-L1 adipocytes

AJP Cell Physiology ◽

10.1152/ajpcell.00097.2017 ◽

2017 ◽

Vol 313 (3) ◽

pp. C295-C304 ◽

Cited By ~ 7

Author(s):

Steven M. Dragos ◽

Karl F. Bergeron ◽

Frédérik Desmarais ◽

Katherine Suitor ◽

David C. Wright ◽

...

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Fatty Acid ◽

White Adipose Tissue ◽

Phosphoenolpyruvate Carboxykinase ◽

Critical Role ◽

Wild Type ◽

Important Regulator ◽

Specific Regulation

White adipose tissue (WAT) has a critical role in lipid handling. Previous work demonstrated that SCD1 is an important regulator of WAT fatty acid (FA) composition; however, its influence on the various interconnected pathways influencing WAT lipid handling remains unclear. Our objective was to investigate the role of SCD1 on WAT lipid handling using Scd1 knockout (KO) mice and SCD1-inhibited 3T3-L1 adipocytes by measuring gene, protein, and metabolite markers related to FA reesterification, glyceroneogenesis, and lipolysis. Triacylglycerol (TAG) content was higher in inguinal WAT (iWAT) from KO mice compared with wild-type, but significantly lower in epididymal WAT (eWAT). The SCD1 desaturation index was decreased in both WAT depots in KO mice. FA reesterification, as measured with a NEFA:glycerol ratio, was reduced in both WAT depots in KO mice, as well as SCD1-inhibited 3T3-L1 adipocytes. Pck1, Atgl, and Hsl gene expression was reduced in both WAT depots of KO mice, while Pck2 and Pdk4 gene expression showed depot-specific regulation. Pck1, Atgl, and Hsl gene expression was reduced, and phosphoenolpyruvate carboxykinase protein content was ablated, in SCD1-inhibited adipocytes. Our data provide evidence that SCD1 has a broad impact on WAT lipid handling by altering TAG composition in a depot-specific manner, reducing FA reesterification, and regulating markers of lipolysis and glyceroneogenesis.

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Brain site-specific regulation of hedonic intake by orexin and DYN peptides: role of the PVN and obesity

Nutritional Neuroscience ◽

10.1080/1028415x.2020.1840049 ◽

2020 ◽

pp. 1-10

Author(s):

P. Mattar ◽

S. Uribe-Cerda ◽

C. Pezoa ◽

T. Guarnieri ◽

C. M. Kotz ◽

...

Keyword(s):

Site Specific ◽

Brain Site ◽

Specific Regulation

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Single neuron serotonin receptor subtype gene expression correlates with behaviour within and across three molluscan species

Proceedings of The Royal Society B Biological Sciences ◽

10.1098/rspb.2018.0791 ◽

2018 ◽

Vol 285 (1885) ◽

pp. 20180791 ◽

Cited By ~ 4

Author(s):

A. N. Tamvacakis ◽

A. Senatore ◽

P. S. Katz

Keyword(s):

Gene Expression ◽

Serotonin Receptor ◽

Single Neuron ◽

Motor Pattern ◽

Receptor Expression ◽

Receptor Subtype ◽

Marine Mollusc ◽

Pleurobranchaea Californica ◽

Molluscan Species ◽

Daily Changes

The marine mollusc, Pleurobranchaea californica varies daily in whether it swims and this correlates with whether serotonin (5-HT) enhances the strength of synapses made by the swim central pattern generator neuron, A1/C2. Another species, Tritonia diomedea , reliably swims and does not vary in serotonergic neuromodulation. A third species, Hermissenda crassicornis , never produces this behaviour and lacks the neuromodulation. We found that expression of particular 5-HT receptor subtype (5-HTR) genes in single neurons correlates with swimming. Orthologues to seven 5-HTR genes were identified from whole-brain transcriptomes. We isolated individual A1/C2 neurons and sequenced their RNA or measured 5-HTR gene expression using absolute quantitative PCR. A1/C2 neurons isolated from Pleurobranchaea that produced a swim motor pattern just prior to isolation expressed 5-HT2a and 5-HT7 receptor genes, as did all Tritonia samples. These subtypes were absent from A1/C2 isolated from Pleurobranchaea that did not s wim on that day and from Hermissenda A1/C2 neurons. Expression of other receptors was not correlated with swimming. This suggests that these 5-HTRs may mediate the modulation of A1/C2 synaptic strength and play an important role in swimming. Furthermore, it suggests that regulation of receptor expression could underlie daily changes in behaviour as well as evolution of behaviour.

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