Individual housing induces altered immuno-endocrine responses to psychological stress in male mice

2003 ◽  
Vol 28 (4) ◽  
pp. 540-558 ◽  
Author(s):  
A Bartolomucci ◽  
P Palanza ◽  
P Sacerdote ◽  
G Ceresini ◽  
A Chirieleison ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Male Mice ◽  
Individual Housing

Impact of individual housing on experimental behavior of male mice

2008 ◽  
Author(s):  
S. S. Arndt ◽  
M. C. Laarakker ◽  
H. A. van Lith ◽  
F. J. van der Staay ◽  
F. Ohl
Keyword(s):  
Male Mice ◽  
Individual Housing ◽  
Experimental Behavior

Restraint stress of male mice triggers apoptosis in spermatozoa and spermatogenic cells via activating the TNF-α system

Zygote ◽  
2020 ◽  
Vol 28 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Jie Zhang ◽  
De-Ling Kong ◽  
Bin Xiao ◽  
Hong-Jie Yuan ◽  
Qiao-Qiao Kong ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Restraint Stress ◽  
Semen Quality ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Testicular Cells ◽  
Tnf Α

SummaryStudies have indicated that psychological stress impairs human fertility and that various stressors can induce apoptosis of testicular cells. However, the mechanisms by which psychological stress on males reduces semen quality and stressors induce apoptosis in testicular cells are largely unclear. Using a psychological (restraint) stress mouse model, we tested whether male psychological stress triggers apoptosis of spermatozoa and spermatogenic cells through activating tumour necrosis factor (TNF)-α signalling. Wild-type or TNF-α−/− male mice were restrained for 48 h before examination for apoptosis and expression of TNF-α and TNF receptor 1 (TNFR1) in spermatozoa, epididymis, seminiferous tubules and spermatogenic cells. The results showed that male restraint significantly decreased fertilization rate and mitochondrial membrane potential, while increasing levels of malondialdehyde, active caspase-3, TNF-α and TNFR1 in spermatozoa. Male restraint also increased apoptosis and expression of TNF-α and TNFR1 in caudae epididymides, seminiferous tubules and spermatogenic cells. Sperm quality was also significantly impaired when spermatozoa were recovered 35 days after male restraint. The restraint-induced damage to spermatozoa, epididymis and seminiferous tubules was significantly ameliorated in TNF-α−/− mice. Furthermore, incubation with soluble TNF-α significantly reduced sperm motility and fertilizing potential. Taken together, the results demonstrated that male psychological stress induces apoptosis in spermatozoa and spermatogenic cells through activating the TNF-α system and that the stress-induced apoptosis in spermatogenic cells can be translated into impaired quality in future spermatozoa.

Hypothalamic levels of LHRF in male mice of differing social status and from individual housing

1985 ◽  
Vol 11 (3) ◽  
pp. 317-321
Author(s):  
P.P. Vescovi ◽  
G. Valenti ◽  
M. Mainardi ◽  
L. Brocchieri ◽  
P.F. Brain
Keyword(s):  
Social Status ◽  
Male Mice ◽  
Individual Housing

Restraint stress of male mice induces apoptosis in spermatozoa and spermatogenic cells: role of the FasL/Fas system†

2019 ◽  
Vol 101 (1) ◽  
pp. 235-247 ◽  
Author(s):  
Bin Xiao ◽  
Xiao Li ◽  
Xiu-Yun Feng ◽  
Shuai Gong ◽  
Zhi-Bin Li ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Semen Quality ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Loss Of Function ◽  
Tnf Α ◽  
Induced Apoptosis ◽  
Necrosis Factor

AbstractThe mechanisms by which psychological stress impairs semen quality are largely unknown. By using a restraint-stressed mouse model, we studied the role of the FasL/Fas system in psychological stress-induced apoptosis of spermatozoa and spermatogenic cells. Male mice were restrained for 48 h before examination for sperm fertilizing potential and for apoptosis and FasL/Fas expression in spermatozoa, spermatogenetic cells/seminiferous tubules, and caudae epididymides. The results showed that the male restraint reduced motility, fertilization rates, and mitochondrial membrane potential while increasing apoptosis and Fas expression in spermatozoa. Restraint also facilitated apoptosis and FasL/Fas expression in spermatogenic cells/seminiferous tubules and caudae epididymides. The restraint-induced apoptosis in spermatozoa and spermatogenic cells was significantly ameliorated in gld mice that harbor a loss-of-function mutation in FasL. However, incubation with FasL did not affect sperm motility and apoptosis, while incubation with tumor necrosis factor (TNF)-α did. The epididymis of the gld mice produced significantly less TNF-α and TNF-related apoptosis-inducing ligand (TRAIL) than that of wild-type mice did after male restraint. Thus, the results confirmed that the FasL/Fas system played an important role in the psychological stress-induced apoptosis of spermatozoa and spermatogenic cells and that FasL triggered sperm apoptosis in epididymis dependently through promoting TNF-α and TRAIL secretion.

A high fat diet-induced decrease in hippocampal newly-born neurons of male mice is exacerbated by mild psychological stress using a Communication Box

2017 ◽  
Vol 209 ◽  
pp. 209-216 ◽  
Author(s):  
Yusuke Murata ◽  
Yukiyasu Narisawa ◽  
Rima Shimono ◽  
Hiraku Ohmori ◽  
Masayoshi Mori ◽  
...  
Keyword(s):  
Psychological Stress ◽  
High Fat Diet ◽  
Male Mice ◽  
High Fat

scholarly journals To Group or Not to Group? Good Practice for Housing Male Laboratory Mice

2017 ◽  
Author(s):  
Sarah Kappel ◽  
Penny Hawkins ◽  
Michael T. Mendl
Keyword(s):  
Life Experiences ◽  
Good Practice ◽  
Social Contact ◽  
Future Research ◽  
Male Mice ◽  
Laboratory Mice ◽  
Individual Housing ◽  
Mouse Behaviour ◽  
Essential Question ◽  
Welfare Problem

It is widely recommended to group house male laboratory mice because they are ‘social animals’, but male mice do not naturally share territories and aggression can be a serious welfare problem. Even without aggression, not all animals within a group will be in a state of positive welfare. Rather, many male mice may be negatively affected by the stress of repeated social defeat and subordination, raising concerns about welfare and also research validity. However, individual housing may not be an appropriate solution, given the welfare implications associated with no social contact. An essential question is whether it is in the best welfare interests of male mice to be group- or singly-housed. This review explores the likely impacts, positive and negative, of both housing conditions, presents results of a survey of current practice and awareness of mouse behaviour, and includes recommendations for good practice and future research. We conclude that whether group- or single-housing is better (or less worse) in any situation is highly context-dependent according to several factors including strain, age, social position, life experiences, and housing and husbandry protocols. It is important to recognise this and evaluate what is preferable from animal welfare and ethical perspectives in each case.

88 The Hypophagia After Psychological Stress in Aged Male Mice Is Mediated by an Interaction of Activation of Estrogen Receptor α With Serotonin 2C Receptor

2015 ◽  
Vol 148 (4) ◽  
pp. S-25-S-26
Author(s):  
Shunsuke Ohnishi ◽  
Koji Nakagawa ◽  
Naoto Okubo ◽  
Chiharu Sadakane ◽  
Yayoi Saegusa ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Psychological Stress ◽  
Estrogen Receptor Α ◽  
Male Mice ◽  
Serotonin 2C Receptor

Ultrastructural Lesions Produced by Alcohol and Sucrose in the Heart and Liver of C57BL Mice

1970 ◽  
Vol 28 ◽  
pp. 208-209
Author(s):  
K.K. SEKHRI ◽  
C.S. ALEXANDER ◽  
H.T. NAGASAWA
Keyword(s):  
Osmium Tetroxide ◽  
Male Mice ◽  
Alcohol Group ◽  
Group Iii ◽  
Group I ◽  
C57bl Mice ◽  
Group Ii

C57BL male mice (Jackson Lab., Bar Harbor, Maine) weighing about 18 gms were randomly divided into three groups: group I was fed sweetened liquid alcohol diet (modified Schenkl) in which 36% of the calories were derived from alcohol; group II was maintained on a similar diet but alcohol was isocalorically substituted by sucrose; group III was fed regular mouse chow ad lib for five months. Liver and heart tissues were fixed in 2.5% cacodylate buffered glutaraldehyde, post-fixed in 2% osmium tetroxide and embedded in Epon-araldite.

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