Digestion kinetic parameters from an in vitro gas production method as predictors of voluntary intake of forage by mature ewes

The associative effects (AE) between concentrate (C), peanut shell (P) and alfalfa (A) were investigated by means of an automated gas production (GP) system. The C, P and A were incubated alone or as 40 : 60 : 0, 40 : 45 : 15, 40 : 30 : 30, 40 : 15 : 45, 40 : 0 : 60 and 30 : 70 : 0, 30 : 55 : 15, 30 : 40 : 30, 30 : 25 : 45, 30 : 10 : 60, 30 : 0 : 70 mixtures where the C : roughage (R) ratios were 40 : 60 and 30 : 70. Samples (0.2000 ± 0.0010 g) of single feeds or mixtures were incubated for 96 h in individual bottles (100 ml) with 30 ml of buffered rumen fluid. GP parameters were analysed using a single exponential equation. After incubation, the residues were used to determine pH, dry matter digestibility (DMD), organic matter digestibility (OMD), volatile fatty acids (VFA) and ammonia nitrogen (NH3-N) of the incubation fluid, and their single factor AE indices (SFAEI) and multiple-factors AE indices (MFAEI) were determined. The results showed that group of 30 peanut shell had higher SFAEI of GP48 h, DMD, OMD and total volatile fatty acids (p < 0.05) and MFAEI (p < 0.05) than groups 60, 45 and 0 when C : R was 40 : 60. The group of 10 peanut shell showed higher SFAEI of GP48 h, DMD and OMD (p < 0.05) than groups 70, 55 and 40 and MFAEI (p < 0.01) when C : R was 30 : 70. It is concluded that optimal SFAEI and MFAEI were obtained when the C : P : A ratios were 40 : 30 : 30 and 30 : 10 : 60.

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Use of an in vitro gas production method to investigate interactions between veld hay and Napier hay or groundnut hay supplements

Animal Feed Science and Technology ◽

10.1016/s0377-8401(97)00011-4 ◽

1997 ◽

Vol 67 (4) ◽

pp. 265-278 ◽

Cited By ~ 3

Author(s):

C.D. Wood ◽

B. Manyuchi

Keyword(s):

Production Method ◽

Gas Production ◽

In Vitro Gas Production

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Effect of vitex agnus-castus on sheep rumen fluids using an in vitro gas production method

10.7287/peerj.preprints.908 ◽

2015 ◽

Author(s):

Sibel S Önenç

Keyword(s):

Organic Matter ◽

Production Method ◽

Gas Production ◽

Net Energy ◽

Alfalfa Hay ◽

In Vitro Gas Production ◽

Agnus Castus ◽

Sheep Rumen ◽

Different Levels

The objective of this study was to evaluate the effects of different levels of Vitex agnus-castus (VAC) on in vitro gas production (GP), organic matter digestibility (OMD) and net energy lactation (NEL) using an in vitro gas-production method. Two rumen-fistulated sheep were used in the experiment. The sheep were fed 60% alfalfa hay and 40% concentrate feed twice daily. Five different levels of VAC were added to the concentrate (CON) to produce 200 mg DM (CON - without VAC; 1 - 2 mg VAC+198 mg CON; 2 - 4 mg VAC+196 mg CON; 3 - 6 mg VAC+194 mg CON; 4 - 8 mg VAC+192 mg CON; 5 - 10 mg VAC+190 mg CON). The volume of gas produced was recorded at 2, 4, 8, 12, and 24 h after incubation. The results showed GP, OMD and NEL contents with no significant changes even as the level of VAC were added to the CON. VAC groups affected the rapidly degraded fractions; however, did not affect the slowly degraded fractions.

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Effect of vitex agnus-castus on sheep rumen fluids using an in vitro gas production method

10.7287/peerj.preprints.908v1 ◽

2015 ◽

Author(s):

Sibel S Önenç

Keyword(s):

Organic Matter ◽

Production Method ◽

Gas Production ◽

Net Energy ◽

Alfalfa Hay ◽

In Vitro Gas Production ◽

Agnus Castus ◽

Sheep Rumen ◽

Different Levels

The objective of this study was to evaluate the effects of different levels of Vitex agnus-castus (VAC) on in vitro gas production (GP), organic matter digestibility (OMD) and net energy lactation (NEL) using an in vitro gas-production method. Two rumen-fistulated sheep were used in the experiment. The sheep were fed 60% alfalfa hay and 40% concentrate feed twice daily. Five different levels of VAC were added to the concentrate (CON) to produce 200 mg DM (CON - without VAC; 1 - 2 mg VAC+198 mg CON; 2 - 4 mg VAC+196 mg CON; 3 - 6 mg VAC+194 mg CON; 4 - 8 mg VAC+192 mg CON; 5 - 10 mg VAC+190 mg CON). The volume of gas produced was recorded at 2, 4, 8, 12, and 24 h after incubation. The results showed GP, OMD and NEL contents with no significant changes even as the level of VAC were added to the CON. VAC groups affected the rapidly degraded fractions; however, did not affect the slowly degraded fractions.

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Relationships between in vitro gas production characteristics and composition of tree leaf fodders from bolivia, west africa and colombia

Proceedings of the British Society of Animal Science ◽

10.1017/s0308229600028816 ◽

1995 ◽

Vol 1995 ◽

pp. 115-115

Author(s):

C D Wood ◽

C Grillet ◽

M Rosales ◽

S Green

Keyword(s):

West Africa ◽

Nutritive Value ◽

Developed Countries ◽

Production Method ◽

Gas Production ◽

In Vitro Gas Production ◽

Wide Range ◽

Tree Leaf ◽

Production Characteristics

Tree leaves are important fodders in many less developed countries, particularly in dry seasons when alternative feeds can be scarce. Although many leaves appear to be good quality fodders due to their generally high crude protein content, many contain anti-nutritive factors which reduce their nutritive value. Tannins are the most widespread of the anti-nutritive factors and are to be found in many tree species. An in vitro gas production method has been developed by Theodorou et al (1994) and applied to the ranking of tree leaf fodders (Wood et al, 1993). This paper combines data obtained from three separate pieces of work investigating the nutritive value of tree leaf fodders in Bolivia, West Africa and Colombia. It seeks to investigate relationships between in vitro gas production at various times of incubation and proximate composition, fibre and tannin content in order to identify which components are of particular importance in determining gas production characteristics and whether these relationships are consistent over a wide range of species from different sources.

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PSVI-18 Evaluation of alternative methodologies for dry matter degradability determination in the in vitro gas production method

Journal of Animal Science ◽

10.1093/jas/skaa278.750 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 430-431

Author(s):

Annelise G Lobo ◽

Rafaela Vincenzi ◽

Richard R Lobo ◽

Vanderlei Benetel Junior ◽

Leticia L Panosso ◽

...

Keyword(s):

Dry Matter ◽

Production Method ◽

Gas Production ◽

Alternative Methods ◽

P Value ◽

In Vitro Gas Production ◽

Filtration System ◽

Randomized Experimental Design ◽

Time Required

Abstract The use of filter crucibles to determine the degradability increases the time required in the analytical process, thus reducing the speed of obtaining experimental data. This has made it necessary to search for alternative methods that ensure a homogeneous digestion and filtration system for all samples and provide accurate and accurate results. The study evaluated the in vitro dry matter degradability of five treatments: 1) Filter crucible; 2) F57 bags (Ankom®) with non-degradable mass; 3) TNT bags weighing g/m2 with non-degradable mass; 4) F57 bags (Ankom®); 5) TNT bags weighing g/m2. The in vitro gas production method used was the semiautomatic one with pressure transducer, for that 8 bovines Nellore castrated male were used as content and ruminal liquid donors. A completely randomized experimental design was used, with 5 treatments, 4 blocks (inocula), and three replicates within each block. PROC GLM was used to adjust for multiple comparisons of the data using the Tukey’s test at 5% significance. There was no difference in the in vitro dry matter degradability for 24 or 96 hours (P-value 0.0554 and 0.1411, respectively), regardless of the treatment. Thus, the possibility of using TNT made bag to the detriment of the F57 bag is affirmed because of the high cost of this one in relation to that one.

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