The forkhead gene family of Caenorhabditis elegans

Gene ◽  
2003 ◽  
Vol 304 ◽  
pp. 43-55 ◽  
Author(s):  
I Hope
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Forkhead Gene

scholarly journals Differential expression of five tRNA(UAGTrp) amber suppressors in Caenorhabditis elegans.

1988 ◽  
Vol 8 (9) ◽  
pp. 3627-3635 ◽  
Author(s):  
K Kondo ◽  
J Hodgkin ◽  
R H Waterston
Keyword(s):  
Nervous System ◽  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Development Stage ◽  
Base Change ◽  
Wild Type ◽  
Specific Manner ◽  
Flanking Sequences ◽  
Single Base Change ◽  
The Individual

Caenorhabditis elegans has 12 tRNA(UGGTrp) genes as defined by Southern analysis. In order to evaluate the function of the individual members of this multigene family, we sought to recover amber (UAG)-suppressing mutations from reversion experiments with animals carrying amber mutations in a nervous system-affecting gene (unc-13) or a sex-determining gene (tra-3). Revertants were analyzed by Southern blot, exploiting the fact that the CCA to CTA change at the anticodon creates a new XbaI site. Five different members of the tRNATrp gene family were identified as suppressors: sup-7 X, sup-5 III, sup-24 IV, sup-28 X, and sup-29 IV. All five suppressor genes were sequenced and found to encode identical tRNA(UAGTrp) molecules with a single base change (CCA to CTA) at the anticodon compared with their wild-type counterparts. The flanking sequences had only limited homology. The relative expression of these five genes was determined by measuring the efficiencies of suppressers against amber mutations in genes affecting the nervous system, hypodermis, muscle, and sex determination. The results of these cross-suppression tests showed that the five members of the tRNA(Trp) gene family were differentially regulated in a tissue- or development stage-specific manner.

Phylogenetic relationships of the Fox (Forkhead) gene family in the Bilateria

Gene ◽  
2003 ◽  
Vol 316 ◽  
pp. 79-89 ◽  
Author(s):  
Françoise Mazet ◽  
Jr-Kai Yu ◽  
David A. Liberles ◽  
Linda Z. Holland ◽  
Sebastian M. Shimeld
Keyword(s):  
Gene Family ◽  
Phylogenetic Relationships ◽  
Forkhead Gene

FMRFamide-related gene family in the nematode, Caenorhabditis elegans

1998 ◽  
Vol 58 (1-2) ◽  
pp. 103-111 ◽  
Author(s):  
Laura S. Nelson ◽  
Kyuhyung Kim ◽  
John E. Memmott ◽  
Chris Li
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Related Gene ◽  
Nematode Caenorhabditis Elegans

Sequence analysis of the complete Caenorhabditis elegans myosin heavy chain gene family

1989 ◽  
Vol 205 (3) ◽  
pp. 603-613 ◽  
Author(s):  
Nick J. Dibb ◽  
Ichiro N. Maruyama ◽  
Michael Krause ◽  
Jonathan Karn
Keyword(s):  
Caenorhabditis Elegans ◽  
Sequence Analysis ◽  
Gene Family ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Myosin Heavy Chain Gene

Isolation and characterization of a sperm-specific gene family in the nematode Caenorhabditis elegans

1984 ◽  
Vol 4 (3) ◽  
pp. 529-537
Author(s):  
M R Klass ◽  
S Kinsley ◽  
L C Lopez
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Specific Gene ◽  
Cdna Clones ◽  
Major Sperm Protein ◽  
Adult Males ◽  
Flanking Sequence ◽  
Nematode Caenorhabditis Elegans ◽  
Developmentally Regulated ◽  
Isolation And Characterization

The major sperm protein (MSP) of the nematode Caenorhabditis elegans is a low-molecular-weight (15,000) basic protein implicated in the pseudopodial movement of mature spermatozoa. Its synthesis occurs in a specific region of the gonad and is regulated at the level of transcription (M. Klass and D. Hirsh, Dev. Biol. 84:299-312, 1981; S. Ward and M. Klass, Dev. Biol. 92:203-208, 1982; Klass et al., Dev. Biol. 93:152-164, 1982). A developmentally regulated gene family has been identified that codes for this MSP. Whole genomic blots, as well as analysis of genomic clone banks, indicate that there are between 15 and 25 copies of the MSP gene in the nematode genome. Southern blot analysis also indicates that there is no rearrangement or amplification within the MSP gene family during development. No evidence was found of methylation at various restriction sites surrounding the MSP gene family, and similarly, no correlation between methylation and expression was observed. Three distinct members of this MSP gene family have been cloned, and their nucleotide sequences have been determined. Differential screening of a cDNA clone bank made from polyadenylated mRNA from adult males yielded 45 male-specific clones, 32 of which were clones of MSP genes. One of these cDNA clones was found to contain the entire nucleotide sequence for the MSP, including part of the 5' leader and all of the 3' trailing sequence. Genomic clones bearing copies of the MSP genes have been isolated. At least one of the members of this gene family is a pseudogene. Another member of the MSP gene family that has been cloned from genomic DNA contains the entire uninterrupted structural sequence for the MSP in addition to a 5' flanking sequence containing a promoter-like region with the classic TATA box, a sequence resembling the CAAT box, and a putative ribosome-binding sequence. The 3' trailing sequences of the genomic and the cDNA clones contain an AATAAA polyadenylation site.

Differential expression of five tRNA(UAGTrp) amber suppressors in Caenorhabditis elegans

1988 ◽  
Vol 8 (9) ◽  
pp. 3627-3635 ◽  
Author(s):  
K Kondo ◽  
J Hodgkin ◽  
R H Waterston
Keyword(s):  
Nervous System ◽  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Development Stage ◽  
Base Change ◽  
Wild Type ◽  
Specific Manner ◽  
Flanking Sequences ◽  
Single Base Change ◽  
The Individual

Caenorhabditis elegans has 12 tRNA(UGGTrp) genes as defined by Southern analysis. In order to evaluate the function of the individual members of this multigene family, we sought to recover amber (UAG)-suppressing mutations from reversion experiments with animals carrying amber mutations in a nervous system-affecting gene (unc-13) or a sex-determining gene (tra-3). Revertants were analyzed by Southern blot, exploiting the fact that the CCA to CTA change at the anticodon creates a new XbaI site. Five different members of the tRNATrp gene family were identified as suppressors: sup-7 X, sup-5 III, sup-24 IV, sup-28 X, and sup-29 IV. All five suppressor genes were sequenced and found to encode identical tRNA(UAGTrp) molecules with a single base change (CCA to CTA) at the anticodon compared with their wild-type counterparts. The flanking sequences had only limited homology. The relative expression of these five genes was determined by measuring the efficiencies of suppressers against amber mutations in genes affecting the nervous system, hypodermis, muscle, and sex determination. The results of these cross-suppression tests showed that the five members of the tRNA(Trp) gene family were differentially regulated in a tissue- or development stage-specific manner.

A single histone H1 isoform (H1.1) is essential for chromatin silencing and germline development in Caenorhabditis elegans

Development ◽  
2001 ◽  
Vol 128 (7) ◽  
pp. 1069-1080 ◽  
Author(s):  
M.A. Jedrusik ◽  
E. Schulze
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Molecular Mechanisms ◽  
Histone H1 ◽  
Specific Aspect ◽  
Polycomb Group ◽  
Germline Development ◽  
C Elegans ◽  
Proliferation And Differentiation ◽  
Germline Proliferation

In remarkable contrast to somatic cells, the germline of the nematode Caenorhabditis elegans efficiently silences transgenic DNA. The molecular mechanisms responsible for this have been shown to implicate chromatin proteins encoded by the mes genes (Kelly, W. G. and Fire, A. (1998) Development 125, 2451–2456), of which two are the C. elegans homologs of Polycomb Group gene transcriptional repressors. We have analyzed the contribution of the histone H1 gene family to this specific aspect of germ cells in C. elegans. We show with isotype-specific double stranded RNA-mediated interference (RNAi) that a single member of this gene family (H1.1) is essential for the repression of a silenced reporter-transgene in the germline of hermaphrodites and males, whereas no change is found in the somatic expression of this reporter. Additionally, RNA-mediated interference with H1.1 gene expression can cause a phenotype with severe affection of germline proliferation and differentiation in the hermaphrodite, and even sterility (5%-11% penetrance). These and further features observed in histone H1.1 RNAi experiments are also characteristic of the mes phenotype (Garvin, C., Holdeman, R. and Strome, S. (1998) Genetics 148, 167–185), which is believed to result from the desilencing of genes required for somatic differentiation in the germline. Our observations therefore support this interpretation of the mes phenotype and they identify a single histone H1 isoform (H1.1) as a new component specifically involved in chromatin silencing in the germline of C. elegans.

scholarly journals The cisd gene family regulates physiological germline apoptosis through ced-13 and the canonical cell death pathway in Caenorhabditis elegans

2018 ◽  
Vol 26 (1) ◽  
pp. 162-178 ◽  
Author(s):  
Skylar D. King ◽  
Chipo F. Gray ◽  
Luhua Song ◽  
Rachel Nechushtai ◽  
Tina L. Gumienny ◽  
...  
Keyword(s):  
Cell Death ◽  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Cell Death Pathway
Sign in / Sign up

Export Citation Format

Share Document