175 POSTER Nanoengineered antibodies against embryonal carcinoma's SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 demonstrate presence of pluripotent cells in situ, in healthy human bone marrow

2006 ◽  
Vol 32 ◽  
pp. S52
Author(s):  
M. Malecki ◽  
J. Pietruszkiewicz ◽  
M.Z. Ratajczak ◽  
P. Jackiewicz ◽  
A. Cichocki
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Pluripotent Cells ◽  
Healthy Human

scholarly journals A minimal standardized human bone marrow microphysiological system to assess resident cell behavior during normal and pathological processes

2022 ◽  
Author(s):  
Thibault Voeltzel ◽  
Gaëlle Fossard ◽  
Michaël Degaud ◽  
Kevin Geistlich ◽  
Nicolas Gadot ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Viable Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Behavior ◽  
Cell Harvesting ◽  
Resident Cell ◽  
Level Of Analysis

We provide an easy to access microphysiological standardized system approaching the human bone marrow complexity to a first level of analysis by in situ imaging or by viable cell harvesting of processes taking place within this ecosystem.

scholarly journals A unique population of IgG-expressing plasma cells lacking CD19 is enriched in human bone marrow

Blood ◽  
2015 ◽  
Vol 125 (11) ◽  
pp. 1739-1748 ◽  
Author(s):  
Henrik E. Mei ◽  
Ina Wirries ◽  
Daniela Frölich ◽  
Mikael Brisslert ◽  
Claudia Giesecke ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune Responses ◽  
B Cell ◽  
Plasma Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Depletion ◽  
B Cell Depletion ◽  
Healthy Human ◽  
Key Points

Key Points Healthy human BM is enriched for PC lacking CD19 that express a prosurvival and distinctly mature phenotype. CD19− PC resist mobilization into blood during immune responses after vaccination as well as B-cell depletion with rituximab.

In Situ immunophenotyping of lymphocytes in human bone marrow: an immunohistochemical study

2008 ◽  
Vol 71 (3) ◽  
pp. 313-321 ◽  
Author(s):  
H.-P. Horny ◽  
U. Engst ◽  
R. S. Walz ◽  
E. Kaiserling
Keyword(s):  
Bone Marrow ◽  
Immunohistochemical Study ◽  
Human Bone ◽  
Human Bone Marrow

In Situ Recruitment of Human Bone Marrow-Derived Mesenchymal Stem Cells Using Chemokines for Articular Cartilage Regeneration

2015 ◽  
Vol 24 (6) ◽  
pp. 1067-1083 ◽  
Author(s):  
Min Sung Park ◽  
Yun Hee Kim ◽  
Youngmee Jung ◽  
Soo Hyun Kim ◽  
Jong Chul Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Articular Cartilage Regeneration

scholarly journals Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters.

1988 ◽  
Vol 168 (3) ◽  
pp. 1193-1198 ◽  
Author(s):  
S H Lee ◽  
P R Crocker ◽  
S Westaby ◽  
N Key ◽  
D Y Mason ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Transferrin Receptor ◽  
Antigen Expression ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hla Dr

Stromal macrophages (M phi) have been localized in situ and isolated within erythroid clusters from human marrow. Stromal M phi arborize in an extensive network uniformly distributed throughout marrow interstitium, and express the phenotype CD4+, CD11a+, CD11c+, CD13+, CD14+, CD16+, CD18+, CD31+, CD32+, FcRI+, HLA-DR+, and CD35-, transferrin receptor-negative, and CD11b (weak). They express endocytic receptor antigens, but show significant differences in myeloid antigen expression compared with freshly harvested or cultured monocytes. Human stromal M phi are therefore specialized mature marrow M phi that are accessible for further investigations in infectious, storage, or hemopoietic disorders.

Set7 Is Highly Expressed in B-Lineage Acute Lymphoblastic Leukemia Cells and Overexpression of Set7 Exhibits Antileukemia Effect

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5088-5088
Author(s):  
Xiao-Tong Ma ◽  
Ya-Kun Mou ◽  
Yang-Yang Zhao ◽  
Nan Wang ◽  
Xiao-Yan Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Lines ◽  
Molecular Mechanisms ◽  
Cancer Metabolism ◽  
Lymphoblastic Leukemia ◽  
Human Bone ◽  
Molecular Therapy ◽  
Human Bone Marrow ◽  
Protein Sequence Analysis ◽  
Healthy Human

Abstract Set7 is a member of protein lysine methyltransferase family that is highly conserved in vertebrates. Set7 can regulate the maintenance of chromosome structure, cell cycle and apoptosis, and plays an important role in many kinds of cancer, metabolism and inflammatory process. However, studies concerning its pathogenic role in leukemia are scarce. We analyzed the expression of Set7 in different types of leukemia and healthy human bone marrow cells or peripheral blood from the Oncomine databases (http://www.oncomine.org), and showed that Set7 was highly expressed in acute lymphoid leukemia (ALL) patients, especially in patients with B-ALL. In this study, we examined the expression of Set7 in various hematopoietic tumor cell lines, bone marrow samples from patients and healthy volunteers. We found that Set7 was highly expressed in B-ALL cell lines Nalm6 and REH compared with other hematopoietic malignant cell lines. Furthermore, Set7 was overexpressed in bone marrow mononuclear cells of adult and pediatric patients with B-ALL than in healthy human bone marrow samples, and sorted CD19+ cells, as well (P<0.001). To investigate Set7 gene function, retroviral-mediated overexpression or removal experiments were performed. Gain-of-function and loss-of-function analyses in Nalm6 and REH cells demonstrated that Set7 inhibited cell growth, colony formation and enhanced the chemosensitivity of B-ALL cells to Vincristine (VCR), Arabinocytidine (Ara) and Daunorubicin (DNR). Protein sequence analysis showed that Ebf1 might be a potential substrate for Set7. The expression of Rad51 and Tnsf11, the downstream target genes of Ebf1, were changed as expected when Set7 was overexpressed in Nalm6 and REH. Our study shows for the first time that overexpression of Set7 has an inhibitory effect on B-ALL. These findings suggest that Set7 may be a promising molecular therapy target for B-ALL treatment and provide new clues for understanding the molecular mechanisms of the leukemogenesis of B-ALL. Disclosures No relevant conflicts of interest to declare.

Cellular levels of mRNA from c-myc, c-myb and c-fes onc-genes in normal myeloid anderythroid precursors of human bone marrow: an in situ hybridization study

1986 ◽  
Vol 62 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Giovanni Emilia ◽  
Amedea Donelli ◽  
Sergio Ferrari ◽  
Umberto Torelli ◽  
Licia Selleri ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hybridization Study
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