P1346 Seventy Enterobacter cloacae bloodstream infections in central Taiwan during 2001 and 2003: evaluation of the roles of extended-spectrum β-lactamases and class 1 integron

2007 ◽  
Vol 29 ◽  
pp. S373
Author(s):  
C. Chen ◽  
Y.-J. Chang ◽  
C.-E. Liu ◽  
C.-C. Huang
Keyword(s):  
Bloodstream Infections ◽  
Enterobacter Cloacae ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1 ◽  
Central Taiwan

Antimicrobial Resistance, Extended-Spectrum β-Lactamase Productivity, and Class 1 Integrons in Escherichia coli from Healthy Swine

2015 ◽  
Vol 78 (8) ◽  
pp. 1442-1450 ◽  
Author(s):  
KANJANA CHANGKAEW ◽  
APIRADEE INTARAPUK ◽  
FUANGFA UTRARACHKIJ ◽  
CHIE NAKAJIMA ◽  
ORASA SUTHIENKUL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Intestinal Flora ◽  
Class 1 Integron ◽  
Potential Health ◽  
Gene Cassettes ◽  
E Coli ◽  
Class 1 Integrons ◽  
Extended Spectrum ◽  
Class 1

Administration of antimicrobials to food-producing animals increases the risk of higher antimicrobial resistance in the normal intestinal flora of these animals. The present cross-sectional study was conducted to investigate antimicrobial susceptibility and extended-spectrum β-lactamase (ESBL)–producing strains and to characterize class 1 integrons in Escherichia coli in healthy swine in Thailand. All 122 of the tested isolates had drug-resistant phenotypes. High resistance was found to ampicillin (98.4% of isolates), chloramphenicol (95.9%), gentamicin (78.7%), streptomycin (77.9%), tetracycline (74.6%), and cefotaxime (72.1%). Fifty-four (44.3%) of the E. coli isolates were confirmed as ESBL-producing strains. Among them, blaCTX-M (45 isolates) and blaTEM (41 isolates) were detected. Of the blaCTX-M-positive E. coli isolates, 37 carried the blaCTX-M-1 cluster, 12 carried the blaCTX-M-9 cluster, and 5 carried both clusters. Sequence analysis revealed blaTEM-1, blaTEM-135, and blaTEM-175 in 38, 2, and 1 isolate, respectively. Eighty-seven (71%) of the 122isolates carried class 1 integrons, and eight distinct drug-resistance gene cassettes with seven different integron profiles were identified in 43 of these isolates. Gene cassettes were associated with resistance to aminoglycosides (aadA1, aadA2, aadA22, or aadA23), trimethoprim (dfrA5, dfrA12, or dfrA17), and lincosamide (linF). Genes encoding β-lactamases were not found in class 1 integrons. This study is the first to report ESBL-producing E. coli with a class 1 integron carrying the linF gene cassette in swine in Thailand. Our findings confirm that swine can be a reservoir of ESBL-producing E. coli harboring class 1 integrons, which may become a potential health risk if these integrons are transmitted to humans. Intensive analyses of animal, human, and environmental isolates are needed to control the spread of ESBL-producing E. coli strains.

scholarly journals Characterization of In111, a class 1 integron that carries the extended-spectrum β-lactamase geneblaIBC-1

2003 ◽  
Vol 225 (1) ◽  
pp. 149-153 ◽  
Author(s):  
S. Vourli ◽  
L.S. Tzouvelekis ◽  
E. Tzelepi ◽  
E. Lebessi ◽  
N.J. Legakis ◽  
...  
Keyword(s):  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1

scholarly journals The aadB Gene Cassette Is Associated with blaSHV Genes in Klebsiella Species Producing Extended-Spectrum β-Lactamases

2005 ◽  
Vol 49 (2) ◽  
pp. 794-797 ◽  
Author(s):  
Louisa A. Jones ◽  
Christopher J. McIver ◽  
Mi-Jurng Kim ◽  
William D. Rawlinson ◽  
Peter A. White
Keyword(s):  
Gene Cassette ◽  
Amplicon Sequencing ◽  
Klebsiella Species ◽  
Class 1 Integron ◽  
Beta Lactamases ◽  
Gene Cassettes ◽  
Extended Spectrum ◽  
Class 1 ◽  
Extended Spectrum Beta Lactamases

ABSTRACT Integrons were detected in 37 (72.5%) of 51 Klebsiella spp. producing extended-spectrum beta-lactamases by PCR with primers that targeted integrase genes and cassette regions. PCR and amplicon sequencing of the cassette regions revealed aadB and aadA2 gene cassettes that confer resistance to a range of aminoglycosides. aadB was associated with a class 1 integron on a 28-kb plasmid, pES1, that also contained bla SHV-12 and IS26.

scholarly journals Genetic and Enzymatic Properties of Metallo-β-Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae

2005 ◽  
Vol 49 (10) ◽  
pp. 4400-4403 ◽  
Author(s):  
Gulcin G. Gacar ◽  
Kenan Midilli ◽  
Fetiye Kolayli ◽  
Kivanc Ergen ◽  
Sibel Gundes ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Single Gene ◽  
Enterobacter Cloacae ◽  
Gene Cassette ◽  
Enzymatic Properties ◽  
Class 1 Integron ◽  
Hindiii Fragment ◽  
Clinical Strains ◽  
Class 1

ABSTRACT A VIM-5-producing Enterobacter cloacae isolate (EDV/1) was identified in a collection of clinical strains stored before 2002. The gene, bla VIM-5, was located on a 2,712-bp BamHI-HindIII fragment of a 23-kbp (approximately) nonconjugative plasmid (pEDV5) in a class 1 integron as a single gene cassette.

scholarly journals Detection and Characterization of VIM-31, a New Variant of VIM-2 with Tyr224His and His252Arg Mutations, in a Clinical Isolate of Enterobacter cloacae

2012 ◽  
Vol 56 (6) ◽  
pp. 3283-3287 ◽  
Author(s):  
Pierre Bogaerts ◽  
Carine Bebrone ◽  
Te-Din Huang ◽  
Warda Bouchahrouf ◽  
Yves DeGheldre ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Enterobacter Cloacae ◽  
Colonic Adenocarcinoma ◽  
Beta Lactam ◽  
Class 1 Integron ◽  
Content Type ◽  
New Variant ◽  
Class 1 ◽  
Blood Specimens

ABSTRACTWe report the first description of the metallo-β-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, inEnterobacter cloacae11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium.blaVIM-31was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lowerkcat(except for ertapenem) and higherKmvalues for VIM-31.

scholarly journals Characterization of multidrug-resistant and extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals

2009 ◽  
Vol 58 (11) ◽  
pp. 1463-1469 ◽  
Author(s):  
King Ting Lim ◽  
Chew Chieng Yeo ◽  
Rohani Md Yasin ◽  
Ganeswrie Balan ◽  
Kwai Lin Thong
Keyword(s):  
Klebsiella Pneumoniae ◽  
Dna Fingerprinting ◽  
Antibiotic Resistance Gene ◽  
Multidrug Resistant ◽  
Pcr Detection ◽  
Management Problem ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1 ◽  
Encoding Genes

The emergence of multidrug-resistant (MDR) and extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae poses a serious antibiotic management problem as resistance genes are easily transferred from one organism to another. Fifty-one strains of K. pneumoniae isolated from sporadic cases in various hospitals throughout Malaysia were analysed by antimicrobial susceptibility testing, PCR detection of ESBL-encoding genes and DNA fingerprinting. Although 27 of the 51 K. pneumoniae strains were MDR (i.e. resistant to three or more classes of antibiotics), the majority of the strains (98 %) were sensitive to imipenem. PCR detection using ESBL gene-specific primers showed that 46 of the K. pneumoniae strains harboured bla SHV, 19 harboured bla CTX-M, 5 harboured bla OXA-1 and 4 harboured bla TEM-1. Class 1 integron-encoded intI1 integrase was detected in 21 of the 51 K. pneumoniae strains and amplification of the integron 5′CS region showed the presence of several known antibiotic resistance gene cassettes of various sizes. Results of conjugation and transformation experiments indicated that some of the ESBL-encoding genes (i.e. bla SHV, bla CTX-M and bla TEM-1) were transmissible and were likely plasmid-encoded. DNA fingerprinting using PFGE and PCR-based methods indicated that the 51 K. pneumoniae strains were genetically diverse and heterogeneous.

scholarly journals Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center

2021 ◽  
Vol 15 (05) ◽  
pp. 657-664
Author(s):  
Iliana Alejandra Cortés-Ortíz ◽  
Julio Cesar Juárez-Gómez ◽  
Concepción Cu-Quijano ◽  
Rocio Flores-Paz ◽  
Emilio Mariano Durán-Manuel ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Variable Region ◽  
Epidemiological Surveillance ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
High Morbidity ◽  
Class 1 Integron ◽  
Genetic Characteristics ◽  
Extended Spectrum ◽  
Class 1

Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak. Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed. Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”. Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel.

scholarly journals Complex Genetic Structures with Repeated Elements, a sul-Type Class 1 Integron, and the blaVEB Extended-Spectrum β-Lactamase Gene

2006 ◽  
Vol 50 (5) ◽  
pp. 1745-1752 ◽  
Author(s):  
Thierry Naas ◽  
Daniel Aubert ◽  
Thierry Lambert ◽  
Patrice Nordmann
Keyword(s):  
Direct Repeat ◽  
Aminoglycoside Resistance ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Conserved Sequence ◽  
Extended Spectrum ◽  
Class 1 ◽  
Genetic Structures ◽  
Lactamase Gene ◽  
First Time

ABSTRACT Two clinical isolates of Pseudomonas aeruginosa, TL-1 and TL-2, were isolated from a patient transferred from Bangladesh and hospitalized for osteomyelitis in Paris, France. P. aeruginosa TL-1 expressed the extended-spectrum β-lactamase VEB-1a and was susceptible only to imipenem and colistin, while P. aeruginosa TL-2 expressed only the naturally occurring bla AmpC gene at a basal level and exhibited a wild-type β-lactam resistance phenotype. In TL-1, the typical 5′-end conserved sequence (5′-CS) region of class 1 integrons usually present upstream of the bla VEB-1a gene was replaced by a truncated 3′-CS and a 135-bp repeated element (Re). Downstream of the bla VEB-1a gene, an insertion sequence, ISPa31 disrupted by ISPa30, and an orf513 sequence, belonging to a common region (conserved region 1 [CR1]) immediately upstream of the aphA-6 gene, were present. Further downstream, a second truncated 3′-CS region in direct repeat belonged to In51, an integron containing two gene cassettes (aadA6 and the OrfD cassette). Thus, the overall structure corresponded to a sul-type class 1 integron termed In121. Genetic analyses revealed that both isolates were clonally related and differed by a ca. 100-kb fragment that contained In121. Both isolates contained another integron, In122, that carried three gene cassettes: aadB, dfrA1, and the OrfX cassette. This work identifies for the first time the spread of Re-associated bla VEB genes located on a sul-type integron. It also reports for the first time a CR1 element in P. aeruginosa that is associated with an aminoglycoside resistance aphA-6 gene that is expressed from a composite promoter.

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