scholarly journals Genetic and Enzymatic Properties of Metallo-β-Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae

2005 ◽  
Vol 49 (10) ◽  
pp. 4400-4403 ◽  
Author(s):  
Gulcin G. Gacar ◽  
Kenan Midilli ◽  
Fetiye Kolayli ◽  
Kivanc Ergen ◽  
Sibel Gundes ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Single Gene ◽  
Enterobacter Cloacae ◽  
Gene Cassette ◽  
Enzymatic Properties ◽  
Class 1 Integron ◽  
Hindiii Fragment ◽  
Clinical Strains ◽  
Class 1

ABSTRACT A VIM-5-producing Enterobacter cloacae isolate (EDV/1) was identified in a collection of clinical strains stored before 2002. The gene, bla VIM-5, was located on a 2,712-bp BamHI-HindIII fragment of a 23-kbp (approximately) nonconjugative plasmid (pEDV5) in a class 1 integron as a single gene cassette.

scholarly journals GES-13, a β-Lactamase Variant Possessing Lys-104 and Asn-170 in Pseudomonas aeruginosa

2010 ◽  
Vol 54 (3) ◽  
pp. 1331-1333 ◽  
Author(s):  
S. D. Kotsakis ◽  
C. C. Papagiannitsis ◽  
E. Tzelepi ◽  
N. J. Legakis ◽  
V. Miriagou ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Broad Spectrum ◽  
Potent Inhibitor ◽  
Single Gene ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Class 1

ABSTRACT GES-13 β-lactamase, a novel GES variant possessing Lys-104 and Asn-170, was identified in Pseudomonas aeruginosa. bla GES-13 was the single gene cassette of a class 1 integron probably located in the chromosome. GES-13 efficiently hydrolyzed broad-spectrum cephalosporins and aztreonam. Imipenem was a potent inhibitor of GES-13 but was not hydrolyzed at measurable rates.

scholarly journals attI1-Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence

2016 ◽  
Vol 61 (3) ◽  
Author(s):  
Costas C. Papagiannitsis ◽  
Leonidas S. Tzouvelekis ◽  
Eva Tzelepi ◽  
Vivi Miriagou
Keyword(s):  
Single Gene ◽  
Gene Cassette ◽  
Open Reading Frames ◽  
Translation Initiation Region ◽  
Class 1 Integron ◽  
Reading Frame ◽  
Class 1 ◽  
Shine Dalgarno Sequence ◽  
Reading Frames ◽  
Small Open Reading Frames

ABSTRACT By searching the Integrall integron and GenBank databases, a novel open reading frame (ORF) of 51 nucleotides (nts) (ORF-17) overlapping the previously described ORF-11 was identified within the attI1 site in virtually all class 1 integrons. Using a set of isogenic plasmid constructs carrying a single gene cassette (bla GES-1) and possessing a canonical translation initiation region, we found that ORF-17 contributes to GES-1 expression.

scholarly journals Integron-Located oxa-32 Gene Cassette Encoding an Extended-Spectrum Variant of OXA-2 β-Lactamase from Pseudomonas aeruginosa

2002 ◽  
Vol 46 (2) ◽  
pp. 566-569 ◽  
Author(s):  
Laurent Poirel ◽  
Patrick Gerome ◽  
Christophe De Champs ◽  
Jean Stephanazzi ◽  
Thierry Naas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Amino Acid ◽  
Clinical Isolate ◽  
Amino Acid Substitution ◽  
Gene Cassette ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Class 1 Integron ◽  
Class D ◽  
Class 1

ABSTRACT Pseudomonas aeruginosa clinical isolate CY-1, which was resistant to ceftazidime, harbored a conjugative ca. 250-kb plasmid that contained a class 1 integron with two gene cassettes encoding OXA-32, an OXA-2- type β-lactamase, and the aminoglycoside acetyltransferase AAC(6′)Ib9. OXA-32 differed from OXA-2 by an Leu169Ile amino acid substitution (class D numbering). Site-directed mutagenesis established that Ile169 is responsible for resistance to ceftazidime but not to cefotaxime.

scholarly journals The Occurrence and Characterization of Class I, II, and III Integrons Among Carbapenemase-Producing Clinical Strains of Acinetobacter baumannii in Tehran, Iran

2021 ◽  
Vol 14 (6) ◽  
Author(s):  
Omid Azizi ◽  
Sepideh Fereshteh ◽  
Omid Nasiri ◽  
Mohammad Ghorbani ◽  
Seyed Mahmoud Barzi ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Clinical Setting ◽  
Gene Cassette ◽  
Predictive Biomarker ◽  
Multidrug Resistant ◽  
High Morbidity ◽  
Class 1 Integron ◽  
Clinical Strains ◽  
Class 1 ◽  
Dna Elements

Background: Acinetobacter baumannii has emerged as a critical pathogen with high morbidity and mortality in long-term hospitalized patients who stay in intensive care units. Carbapenemases and integrons are two critical DNA elements that contribute to the emergence of multidrug-resistant (MDR) A. baumannii. Objectives: The current study aimed at characterization and molecular detection of class 1, 2, and 3 integrons among carbapenemase-producing A. baumannii strains recovered from a clinical setting in Tehran, Iran. Methods: A total of 65 non-replicated clinical strains were considered in this study. Class 1, 2, and 3 carbapenemase genes and clonal relatedness of the isolates were investigated by PCR assay. Results: The prevalence of carbapenemases was as follows: blaOXA23 (92.31%), blaVIM (69.23%), and blaNDM (1.54%). In addition, the gene cassette arrays consisted of aacA4-catB8-aadA1 (12/46, 26.09%), aadB-aadA1 (26.09%, 12/46), arr2-cm1A5 (30.43%, 14/46), and dfrA1-aadA1 (7.39%, 8/46) in class 1 integron and dfrA1-sat2 (52.94%, 9/17) and sat2-aadA1 (47.06%, 8/17) in class 2 integron. Sequence-based typing of both blaOXA-51-like and ampC revealed the following distribution of three different clone types among isolates: clonal complex (CC) 10 (46.15%, 30/65), CC2 (40%, 26/65), and CC3 (13.85%, 9/65). Statistical analysis showed that the presence of the intI1, blaOXA23, blaVIM, or blaNDM genes can significantly increase the acquiring MDR phenotypes in A. baumannii isolates. Conclusions: High prevalence of carbapenemase-producing A. baumannii harboring integrons is alarming public health. It seems that class 1 integron can be served as a predictive biomarker for the presence of MDR bacteria in the clinical setting. However, integrons do not carry carbapenemases in these strains.

scholarly journals A Novel Gene Cassette,aacA43, in a Plasmid-Borne Class 1 Integron

2011 ◽  
Vol 55 (6) ◽  
pp. 2979-2982 ◽  
Author(s):  
Sally R. Partridge ◽  
Lee C. Thomas ◽  
Andrew N. Ginn ◽  
Agnieszka M. Wiklendt ◽  
Pierre Kyme ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Enterobacter Cloacae ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Content Type ◽  
Novel Gene ◽  
Class 1

ABSTRACTA novel gene cassette,aacA43, was identified in theaadB-aacA43-oxa10-smr2cassette array in a class 1 integron. Like related aminoglycoside-(6′)-acetyltransferases, AacA43 confers clinically relevant resistance to kanamycin, tobramycin, and some less-used aminoglycosides but not to gentamicin. Although transferable on an IncL/M plasmid,aacA43was identified in only two differentKlebsiella pneumoniaestrains (14 isolates), oneEscherichia colistrain (2 isolates), and oneEnterobacter cloacaestrain in a survey of patients in a Sydney intensive care unit in 2004-2005.

scholarly journals Characterization of a Novel Plasmid-Mediated Cephalosporinase (CMY-9) and Its Genetic Environment in an Escherichia coli Clinical Isolate

2002 ◽  
Vol 46 (8) ◽  
pp. 2427-2434 ◽  
Author(s):  
Yohei Doi ◽  
Naohiro Shibata ◽  
Keigo Shibayama ◽  
Kazunari Kamachi ◽  
Hiroshi Kurokawa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Gene Cassette ◽  
Single Amino Acid ◽  
Common Region ◽  
Class 1 Integron ◽  
Class 1 ◽  
The Common ◽  
High Level

ABSTRACT An Escherichia coli strain, HKYM68, which showed resistance to broad-spectrum cephalosporins was isolated from a sputum specimen in Japan. The high-level resistance of the strain to ceftazidime, cefpirome, and moxalactam was carried by a self-transferable plasmid. The β-lactamase gene responsible for the resistance was cloned and sequenced. The deduced amino acid sequence of this gene product, CMY-9, had a single amino acid substitution (E85D), the residue reported to be part of the recognition site for the R1 side chain of β-lactams, compared with the amino acid sequence of CMY-8 and also had 78% identity with the amino acid sequence of CepH, a chromosomal cephalosporinase of Aeromonas hydrophila. A sul1-type class 1 integron containing an aacA1-orfG gene cassette was identified upstream of bla CMY-9 and ended with a truncated 3′ conserved segment. The following 2.1 kb was almost identical to the common region of integrons In6 and In7 and the integron of pSAL-1, except that orf513 encoding a putative transposase was identified instead of orf341 due to addition of a single nucleotide. bla CMY-9 was closely located downstream of the end of the common region. These observations are indicative of the exogenous derivation of bla CMY-9 from some environmental microorganisms such as aeromonads.

Antibiotic resistance profile of Salmonella enterica isolated from exotic and indigenous leafy green vegetables in Accra, Ghana

2021 ◽  
Author(s):  
Jinru Chen ◽  
Joycelyn Quansah
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Gene Cassette ◽  
Nucleotide Sequencing ◽  
Dilution Method ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Class 1

Fresh produce-borne enteric bacterial pathogens with resistance to antibiotics have posed serious challenges to food safety and public health worldwide.  This study examined the antibiotic resistance profile of Salmonella enterica (n=33), previously isolated from exotic and indigenous leafy green vegetable samples (n=328) collected from 50 vegetable farms in 12 farming areas and 37 vegetable sellers in 4 market centers in Accra, Ghana during the period of March 2016 to March 2017, and determined the distribution of integrons among antibiotic-resistant isolates.  The susceptibility of the Salmonella isolates to 12 antibiotics was assayed using the standard disc diffusion assay.  The minimum inhibitory concentrations (MICs) of the five most resisted antibiotics were determined using the twofold macro dilution method.  PCR assay was used to detect the presence of integrons in Salmonella cells, and PCR product with amplified integron gene cassette was purified and sequenced using the Sanger sequencing technology.  The Salmonella isolates used in the study resisted at least one tested antibiotic, and multi-drug resistant (MDR) isolates were 30.3% (10/33).  Most isolates (81.8%) were resistant to sulfisoxazole.  The MICs of tetracycline, cefoxitin, streptomycin, ampicillin, and sulfisoxazole were 16, 32, 64, 64, and > 1,024 µg/ml, respectively.  A total of five different patterns of MDR were observed among the Salmonella isolates, and the common MDR patterns were AAuFox (30.3%) and AAuFoxSSu (18.1%).  One out of the 33 (3.0%) Salmonella isolates tested positive for class 1 integron with a gene cassette of about 800 bp.  Nucleotide sequencing revealed the class 1 integron carried a single gene dfrA7 .  Future studies are needed to confirm whether the consumption of contaminated leafy green vegetables is a route of acquiring antibiotic-resistant Salmonella by consumers in Accra, Ghana.

scholarly journals A Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Gabriele Arcari ◽  
Federica Maria Di Lella ◽  
Giulia Bibbolino ◽  
Fabio Mengoni ◽  
Marzia Beccaccioli ◽  
...  
Keyword(s):  
Host Range ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Gene Cassette ◽  
University Hospital ◽  
Broad Host Range ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACT In this study, we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, blaVIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the blaSHV-12, qnrS1, and mph(A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.

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