Fate of enterotoxigenic Staphylococcus aureus in the presence of nisin-producing Lactococcus lactis strain during manufacture of Jben, a Moroccan traditional fresh cheese

AbstractBackgroundLactococcus lactis strain Plasma (LC-Plasma) was revealed to stimulate plasmacytoid dendritic cells and induce antiviral immunity in vitro and in vivo. In this study, we assessed the effects of LC-Plasma on skin immunity.MethodsTo evaluate the effect of LC-Plasma on skin immunity and Staphylococcus aureus epicutaneous infection, lymphocyte activities in skin-draining lymph nodes (SLNs) and gene expression in skin were analyzed after 2 weeks of oral administration of LC-Plasma. To evaluate the mechanisms of interleukin 17A production, SLN lymphocytes were cultured with or without LC-Plasma, and the interleukin 17A concentrations in supernatants were measured.ResultsOral administration of LC-Plasma activated plasma dendritic cells in SLNs, augmented skin homeostasis, and elicited suppression of Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes proliferation. In addition, significant suppression of the S. aureus burden and reduced skin inflammation were observed following oral administration of LC-Plasma. Furthermore, a subsequent in vitro study revealed that LC-Plasma could elicit interleukin 17A production from CD8+ T cells and that its induction mechanism depended on the Toll-like receptor 9 signaling pathway, with type I interferon partially involved.ConclusionsOur results suggest that LC-Plasma oral administration enhances skin homeostasis via plasma dendritic cell activation in SLNs, resulting in suppression of S. aureus epicutaneous infection and skin inflammation.

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The Effects of Dietary Supplementation of Lactococcus lactis Strain Plasma on Skin Microbiome and Skin Conditions in Healthy Subjects—A Randomized, Double-Blind, Placebo-Controlled Trial

Microorganisms ◽

10.3390/microorganisms9030563 ◽

2021 ◽

Vol 9 (3) ◽

pp. 563

Author(s):

Ryohei Tsuji ◽

Kamiyu Yazawa ◽

Takeshi Kokubo ◽

Yuumi Nakamura ◽

Osamu Kanauchi

Keyword(s):

Gene Expression ◽

Lactococcus Lactis ◽

Healthy Subjects ◽

Controlled Trial ◽

Dietary Supplementation ◽

Skin Microbiome ◽

Double Blind ◽

Double Blind Placebo ◽

Lactis Strain ◽

Skin Conditions

(1) Background: Lactococcus lactis strain Plasma (LC-Plasma) is a unique strain which directly activates plasmacytoid dendritic cells, resulting in the prevention against broad spectrum of viral infection. Additionally, we found that LC-Plasma intake stimulated skin immunity and prevents Staphylococcus aureus epicutaneous infection. The aim of this study was to investigate the effect of LC-Plasma dietary supplementation on skin microbiome, gene expression in the skin, and skin conditions in healthy subjects. (2) Method: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted. Seventy healthy volunteers were enrolled and assigned into two groups receiving either placebo or LC-Plasma capsules (approximately 1 × 1011 cells/day) for 8 weeks. The skin microbiome was analyzed by NGS and qPCR. Gene expression was analyzed by qPCR and skin conditions were diagnosed by dermatologists before and after intervention. (3) Result: LC-Plasma supplementation prevented the decrease of Staphylococcus epidermidis and Staphylococcus pasteuri and overgrowth of Propionibacterium acnes. In addition, LC-Plasma supplementation suggested to increase the expression of antimicrobial peptide genes but not tight junction genes. Furthermore, the clinical scores of skin conditions were ameliorated by LC-Plasma supplementation. (4) Conclusions: Our findings provided the insights that the dietary supplementation of LC-Plasma might have stabilizing effects on seasonal change of skin microbiome and skin conditions in healthy subjects.

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An engineered Lactococcus lactis strain exerts significant immune responses through efficient expression and delivery of Helicobacter pylori Lpp20 antigen

Biotechnology Letters ◽

10.1007/s10529-016-2209-x ◽

2016 ◽

Vol 38 (12) ◽

pp. 2169-2175 ◽

Cited By ~ 10

Author(s):

Rongguang Zhang ◽

Xiaoyan Peng ◽

Guangcai Duan ◽

Qingfeng Shi ◽

Shuaiyin Chen ◽

...

Keyword(s):

Helicobacter Pylori ◽

Immune Responses ◽

Lactococcus Lactis ◽

Efficient Expression ◽

Lactis Strain

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A Survey of Coliforms and Staphylococcus aureus in Cheese Using Impedimetric and Plate Count Methods1

Journal of Food Protection ◽

10.4315/0362-028x-51.1.53 ◽

1988 ◽

Vol 51 (1) ◽

pp. 53-55 ◽

Cited By ~ 8

Author(s):

F. A. KHAYAT ◽

J. C. BRUHN ◽

G. H. RICHARDSON

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Correlation Coefficient ◽

Cheddar Cheese ◽

Plate Count ◽

Impedance Method ◽

Colony Forming Units ◽

Fresh Cheese ◽

Cheese Products ◽

And Staphylococcus Aureus

A total of 256 cheese samples were analyzed for coliform plate count using violet red bile agar and for an impedance count using BactometerR Coliform Medium with a correlation coefficient between methods of R=−.91. Fifty-four percent of the samples contained 102 to 107 colony forming units/gram (CFU/g). The highest counts were in cream and fresh cheese products. When 27 Cheddar cheese samples were inoculated with from 102 to 107 CFU of Escherichia coli/g a correlation of R=−.97 was found between methods. Two hundred of the cheese samples were analyzed for Staphylococcus aureus using Baird-Parker medium and impedance count using BactometerR S.aureus Medium. Five samples (2%) contained over 103 CFU/g. The strains isolated were coagulase-positive. When 34 samples of cheese were inoculated with 102 to 107 CFU of staphylococci/g, the correlation between the plate and impedance method was R=0.98.

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Inhibition of a Methicillin-Resistant Staphylococcus aureus Strain in Afuega'l Pitu Cheese by the Nisin Z–Producing Strain Lactococcus lactis subsp. lactis IPLA 729

Journal of Food Protection ◽

10.4315/0362-028x-67.5.928 ◽

2004 ◽

Vol 67 (5) ◽

pp. 928-933 ◽

Cited By ~ 29

Author(s):

NATALIA RILLA ◽

BEATRIZ MARTÍNEZ ◽

ANA RODRÍGUEZ

Keyword(s):

Staphylococcus Aureus ◽

Lactococcus Lactis ◽

Methicillin Resistant Staphylococcus Aureus ◽

Aureus Strain ◽

Northern Spain ◽

Potential Threat ◽

Methicillin Resistant ◽

Initial Inoculum ◽

Lactococcus Lactis Subsp ◽

Nisin Z

Methicillin-resistant Staphylococcus aureus strains are a potential threat for food safety because foodborne illness caused by methicillin-resistant Staphylococcus aureus has been reported even though these strains were only associated with nosocomial infections until recently. This article focuses on the inhibitory effect of the nisin Z–producing strain Lactococcus lactis subsp. lactis IPLA 729 on the growth of Staphylococcus aureus CECT 4013, a methicillin-resistant strain. S. aureus was inhibited by the presence of the nisin producer IPLA 729 in buffered Trypticase soy broth, milk, and Afuega'l Pitu cheese, an acid-coagulated cheese manufactured in Asturias, Northern Spain. A reduction of 3.66 log units was observed in Trypticase soy broth at the end of the incubation period. In milk, viable counts of S. aureus were undetectable or were reduced by 2.16 log units in 24 h depending on the initial inoculum (1.8 × 104 and 7.2 × 106 CFU/ml). The staphylococcal strain was also undetected in test cheeses in which the nisin Z producer was present whereas 2 log units were detected in control cheeses at the end of ripening.

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Complete Genome Sequence of Lactococcus lactis subsp. lactis Strain 14B4, Which Inhibits the Growth of Salmonella enterica Serotype Poona In Vitro

Microbiology Resource Announcements ◽

10.1128/mra.01364-18 ◽

2018 ◽

Vol 7 (19) ◽

Cited By ~ 1

Author(s):

Thao D. Tran ◽

Steven Huynh ◽

Craig T. Parker ◽

Ruyang Han ◽

Robert Hnasko ◽

...

Keyword(s):

Lactococcus Lactis ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Salmonella Enterica ◽

Complete Genome ◽

Northern California ◽

Content Type ◽

Lactococcus Lactis Subsp ◽

Lactis Strain

We present here the complete genome sequence of Lactococcus lactis strain 14B4, isolated from almond drupes in northern California. This strain was observed to inhibit the growth of Salmonella enterica serotype Poona strain RM3363 in vitro.

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EFFECT OF EXOPOLISACCHARIDES OF LACTIC ACID BACTERIA ON THE SYNTHESIS OF PROINFLAMMATORY CYTOKINES BY MACROPHAGIC MICE IN PHAGOCYTOSIS OF STAPHYLOCOCCUS AUREUS

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-2018-1-67-71 ◽

2018 ◽

pp. 67-71

Author(s):

G. T. Uryadova ◽

E. A. Gorelnikova ◽

N. A. Fokina ◽

A. S. Dolmashkina ◽

L. V. Karpunina

Keyword(s):

Staphylococcus Aureus ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactococcus Lactis ◽

Inflammatory Cytokines ◽

Proinflammatory Cytokines ◽

Streptococcus Thermophilus ◽

Ambiguous Effect ◽

Pro Inflammatory Cytokines

Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 13, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals.

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