In vitro antifungal activity of new series of homoallylamines and related compounds with inhibitory properties of the synthesis of fungal cell wall polymers

2003 ◽  
Vol 11 (7) ◽  
pp. 1531-1550 ◽  
Author(s):  
Leonor Y Vargas M ◽  
Marı́a V Castelli ◽  
Vladimir V Kouznetsov ◽  
Juan M Urbina G ◽  
Silvia N López ◽  
...  
Keyword(s):  
Cell Wall ◽  
Antifungal Activity ◽  
Fungal Cell Wall ◽  
Fungal Cell ◽  
Cell Wall Polymers ◽  
Related Compounds ◽  
In Vitro Antifungal Activity

scholarly journals In vitro and in vivo reactivity to fungal cell wall agents in sarcoidosis

2011 ◽  
Vol 166 (1) ◽  
pp. 87-93 ◽  
Author(s):  
M. Terčelj ◽  
S. Stopinšek ◽  
A. Ihan ◽  
B. Salobir ◽  
S. Simčič ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fungal Cell Wall ◽  
Fungal Cell

scholarly journals Antifungal Activity against Botrytis cinerea of 2,6-Dimethoxy-4-(phenylimino)cyclohexa-2,5-dienone Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 706 ◽  
Author(s):  
Paulo Castro ◽  
Leonora Mendoza ◽  
Claudio Vásquez ◽  
Paz Pereira ◽  
Freddy Navarro ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Wall ◽  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Aromatic Ring ◽  
13C Nmr ◽  
Trametes Versicolor ◽  
Fungal Cell Wall ◽  
Fungal Cell ◽  
1H And 13C Nmr

In this work the enzyme laccase from Trametes versicolor was used to synthetize 2,6-dimethoxy-4-(phenylimino)cyclohexa-2,5-dienone derivatives. Ten products with different substitutions in the aromatic ring were synthetized and characterized using 1H- and 13C-NMR and mass spectrometry. The 3,5-dichlorinated compound showed highest antifungal activity against the phytopathogen Botrytis cinerea, while the p-methoxylated compound had the lowest activity; however, the antifungal activity of the products was higher than the activity of the substrates of the reactions. Finally, the results suggested that these compounds produced damage in the fungal cell wall.

scholarly journals The Glycosylphosphatidylinositol-Anchored DFG Family Is Essential for the Insertion of Galactomannan into the β-(1,3)-Glucan–Chitin Core of the Cell Wall of Aspergillus fumigatus

mSphere ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Laetitia Muszkieta ◽  
Thierry Fontaine ◽  
Rémi Beau ◽  
Isabelle Mouyna ◽  
Marian Samuel Vogt ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Gene Family ◽  
Protein Complexes ◽  
Growth Conditions ◽  
Fungal Cell Wall ◽  
Cross Linking ◽  
Fungal Cell ◽  
Content Type

ABSTRACT The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. At the cell wall level, enzyme activities are involved in postsynthesis polysaccharide modifications such as cleavage, elongation, branching, and cross-linking. Glycosylphosphatidylinositol (GPI)-anchored proteins have been shown to participate in cell wall biosynthesis and specifically in polysaccharide remodeling. Among these proteins, the DFG family plays an essential role in controlling polar growth in yeast. In the filamentous fungus and opportunistic human pathogen Aspergillus fumigatus, the DFG gene family contains seven orthologous DFG genes among which only six are expressed under in vitro growth conditions. Deletions of single DFG genes revealed that DFG3 plays the most important morphogenetic role in this gene family. A sextuple-deletion mutant resulting from the deletion of all in vitro expressed DFG genes did not contain galactomannan in the cell wall and has severe growth defects. This study has shown that DFG members are absolutely necessary for the insertion of galactomannan into the cell wall of A. fumigatus and that the proper cell wall localization of the galactomannan is essential for correct fungal morphogenesis in A. fumigatus. IMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. Enzymes involved in postsynthesis polysaccharide modifications, such as cleavage, elongation, branching, and cross-linking, are essential for fungal life. Here, we investigated in Aspergillus fumigatus the role of the members of the Dfg family, one of the 4 GPI-anchored protein families common to yeast and molds involved in cell wall remodeling. Molecular and biochemical approaches showed that DFG members are required for filamentous growth, conidiation, and cell wall organization and are essential for the life of this fungal pathogen.

Localization of fungal fimbriae by immunocytochemistry in pathogenic and nonpathogenic isolates of Venturia inaequalis

2000 ◽  
Vol 46 (9) ◽  
pp. 800-808 ◽  
Author(s):  
Antonet M Svircev ◽  
Ronald J Smith ◽  
Ting Zhou ◽  
Alan W Day
Keyword(s):  
Cell Wall ◽  
Venturia Inaequalis ◽  
Fungal Cell Wall ◽  
Fungal Mycelium ◽  
Fungal Cell ◽  
Transmission Electron ◽  
Developmental Growth ◽  
Large Central Vacuole ◽  
Cellular Organelles

Pathogenic and nonpathogenic isolates of Venturia inaequalis were grown in liquid culture. Hyphae were treated with two types of fimbrial antiserum (AU- and AV-1) and examined by immunofluorescent microscopy, in order to establish the distribution of fimbrial epitopes in whole cell mounts. The AV-1 antiserum was specific for the glycoprotein subunits while the AU- antiserum was specific for the protein moieties present on the fimbriae of Mycobotryum violaceum. The use of fimbrial antiserum with immunocytochemistry and transmission electron microscopy demonstrated a clear distinction between pathogenic and nonpathogenic isolates of V. inaequalis, based on the appearance of the fungal cell wall and the distribution of fimbrial epitopes labeled with AV-1 antiserum and immunogold complex. In actively growing hyphae of the pathogenic isolate, characterized by distinct cellular organelles, small vacuoles, and lipid bodies, fimbrial epitopes were concentrated in the fungal cell wall and were present minimally on the outer surface. In contrast, actively growing hyphae of the nonpathogenic isolate of V. inaequalis had extensive fine hair-like protrusions in the fungal cell wall which labeled with the AV-1 antiserum and immunogold. The distribution of fimbrial epitopes in V. inaequalis was highly dependent on the developmental growth stage of the fungal mycelium. Aging mycelia in both the pathogenic and nonpathogenic isolates of V. inaequalis were characterized by a large central vacuole and no label. In the pathogenic and nonpathogenic isolates of V. inaequalis grown in vitro, the distribution of fimbrial glycoprotein epitopes provided a more complex profile than that seen in M. violaceum.Key words: fimbriae, immunocytochemistry, Venturia inaequalis, Mycobotryum violaceum.

scholarly journals In-vitro Detection of Phytopathogenic Fungal Cell Wall by Polyclonal Sera Raised Against Trimethyl Chitosan Nanoparticles

2019 ◽  
Vol Volume 14 ◽  
pp. 10023-10033
Author(s):  
Hemant Joshi ◽  
Anshu Malik ◽  
Soumya Aggarwal ◽  
Manoj Munde ◽  
Subhrangsu Sundar Maitra ◽  
...  
Keyword(s):  
Cell Wall ◽  
Chitosan Nanoparticles ◽  
Fungal Cell Wall ◽  
Fungal Cell ◽  
Trimethyl Chitosan

In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

2005 ◽  
Vol 40 (1-2) ◽  
pp. 43-54 ◽  
Author(s):  
K. Yamunarani ◽  
R. Jaganathan ◽  
R. Bhaskaran ◽  
P. Govindaraju ◽  
R. Velazhahan
Keyword(s):  
Antifungal Activity ◽  
Quercus Infectoria ◽  
In Vitro Antifungal Activity
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