scholarly journals Anti-SARS-CoV-2 antibodies in healthy donor plasma pools and IVIG products—an update

2022 ◽  
Vol 22 (1) ◽  
pp. 19
Author(s):  
Carolina Romero ◽  
José-María Díez ◽  
Rodrigo Gajardo
Keyword(s):  
Healthy Donor ◽  
Donor Plasma

scholarly journals Anti-SARS-CoV-2 antibodies in healthy donor plasma pools and IVIG products

2021 ◽  
Author(s):  
Carolina Romero ◽  
José María Díez ◽  
Rodrigo Gajardo
Keyword(s):  
Healthy Donor ◽  
Donor Plasma

scholarly journals Circulating Proteasomes Are Functional and Have a Subtype Pattern Distinct from 20S Proteasomes in Major Blood Cells

2006 ◽  
Vol 52 (11) ◽  
pp. 2079-2086 ◽  
Author(s):  
Annette Zoeger ◽  
Michael Blau ◽  
Karl Egerer ◽  
Eugen Feist ◽  
Burkhardt Dahlmann
Keyword(s):  
Autoimmune Disease ◽  
Human Plasma ◽  
Lupus Erythematosus ◽  
Blood Cells ◽  
Healthy Donor ◽  
Intracellular Protein ◽  
Disease Markers ◽  
Apparent Homogeneity ◽  
Healthy Donors ◽  
Donor Plasma

Abstract Background: 20S proteasomes, the proteolytic core particles of the major intracellular protein degradative pathway, are potential disease markers because they are detectable in human plasma as circulating proteasomes and their concentrations are increased in patients suffering from various diseases. To investigate the origin of circulating proteasomes, we compared some of their features with those of proteasomes isolated from major blood cells. Methods: We isolated circulating proteasomes from the plasma of 2 patients with rheumatoid arthritis and 2 with systemic lupus erythematosus and from human plasma from healthy donors. We purified the proteasomes to apparent homogeneity and then used electron microscopy for imaging and chromatography for subtype spectrum analysis. We compared subtype results with those from 20S proteasomes purified from 4 major blood cell populations. We also tested proteasomes for enzymatic activity and immunosubunit content. Results: Circulating proteasomes from plasma of healthy donors and from patients with autoimmune disease were found to have the same size and shape as erythrocyte proteasomes, be proteolytically active, and contain standard- and immunosubunits. Chromatography revealed 6 circulating proteasome subtype peaks in healthy donor plasma and 7 in patient donor plasma. Proteasomes from erythrocytes had 3 subtype peaks and those of monocytes, T-lymphocytes, and thrombocytes each had 5 different subtype peaks. Conclusion: Circulating proteasomes were intact and enzymatically active in plasma from healthy donors and from patients with autoimmune disease. Because the subtype patterns of circulating proteasomes clearly differ from those of proteasomes from blood cells, these cells cannot be regarded as a major source of circulating proteasomes.

Prevention of microvesiculation by adhesion of buds to the mother cell membrane — A possible anticoagulant effect of healthy donor plasma

2008 ◽  
Vol 7 (3) ◽  
pp. 240-245 ◽  
Author(s):  
Mojca Frank ◽  
Mateja Manček-Keber ◽  
Mojca Kržan ◽  
Snežna Sodin-Šemrl ◽  
Roman Jerala ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mother Cell ◽  
Healthy Donor ◽  
Anticoagulant Effect ◽  
Donor Plasma

Haptoglobin polymorphisms and fucosylation change: possible influence of variation on the identified lung cancer-relevant biomarkers

2020 ◽  
Vol 17 ◽  
Author(s):  
Kongsak Boonyapranai ◽  
Sakaewan Ounjaijean ◽  
Kanokwan Kulprachakarn ◽  
Laddawan Potpromanee ◽  
Miles Chih-Ming Chen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Healthy Donor ◽  
Blood Donors ◽  
Acute Phase Protein ◽  
Biological Properties ◽  
Diagnostic Biomarker ◽  
Lung Cancer Patients ◽  
Healthy Donors ◽  
Phase Protein ◽  
Blotting Technique

Background: Haptoglobin (Hp), an acute-phase protein, is known as a potential diagnostic biomarker in human diseases. Two alleles of Hp (Hp1 and Hp2) exist in humans allowing three phenotypes (Hp1-1, Hp2-1, and Hp2-2), which influence the biophysical and biological properties of Hp. Objective: This work aimed to investigate the variation of serum level and fucosylation change among Hp phenotypes in pa-tients with lung cancer compared to healthy donors. Method: 44 patients with lung cancer and 26 healthy blood donors who lived in the Northern-Thailand region were investi-gated by glycoproteomic procedure. Results: The phenotypic distribution of the Hp (Hp1-1:Hp2-1:Hp2-2) in healthy donors were 0.04:0.38:0.58, while the pa-tient group were 0.09:0.52:0.39. The Hp1 allele frequency of the patients with lung cancer (0.34) was higher than the healthy donor (0.23). Glycoprotein blotting technique represented that the level of serum Hp and its fucosylation were sig-nificantly higher among lung cancer patients compared to those of the healthy donors. However, a downward trend in the fucosylation level from Hp1-1 to Hp2-1, Hp2-2, was seen in the patient group, but varying in the serum Hp level. An N-linked glycan was enzymatically released from discrete Hp multimers of Hp2-1 and Hp2-2 samples. Analysis of glycan pro-filing by MALDI-TOF-MS showed that reduction of the fucosylated glycan was associated with the size of Hp multimers, resulting in the lower level of fucosylation in Hp2-1 and Hp2-2, respectively. Conclusion: Our finding demonstrates that the Hp phenotype is a dependent risk factor for lung cancer and should be incor-porated into further clinical and biochemical investigations of diseases, including lung cancer.

scholarly journals Neutralizing Autoantibodies to Type I Interferons in COVID-19 Convalescent Donor Plasma

2021 ◽  
Author(s):  
Sara E. Vazquez ◽  
Paul Bastard ◽  
Kathleen Kelly ◽  
Adrian Gervais ◽  
Philip J. Norris ◽  
...  
Keyword(s):  
Type I Interferons ◽  
Type I ◽  
Donor Plasma

Cardiovascular risk in 159 934 frequent blood donors while addressing the healthy donor effect

Heart ◽  
2019 ◽  
Vol 105 (16) ◽  
pp. 1260-1265 ◽  
Author(s):  
Karlijn Peffer ◽  
Martin den Heijer ◽  
Wim L A M de Kort ◽  
André L M Verbeek ◽  
Femke Atsma
Keyword(s):  
Cardiovascular Risk ◽  
High Frequency ◽  
Healthy Donor ◽  
Blood Donors ◽  
Cox Regression ◽  
Blood Donation ◽  
Low Frequency ◽  
Sensitivity Analyses ◽  
Hospital Data ◽  
Dutch Hospital

ObjectiveTo investigate whether regular blood donation decreases cardiovascular risk.MethodsAll 159 934 Dutch whole-blood donors with an active donation career of at least 10 years were categorised into sex-specific donation tertiles based on the number of donations during this 10-year qualification period. Cardiovascular endpoints were based on hospital discharge diagnoses and death certificates from Dutch Hospital Data and Statistics Netherlands and occurring after the 10-year qualification period. Cox regression was used to estimate the age-adjusted and starting year-adjusted hazard rate ratio (HRR).ResultsFemale high-frequency blood donors had a reduced cardiovascular morbidity (HRR=0.91, 95% CI 0.85 to 0.98) compared with low-frequency blood donors. No effect was observed in men (HRR=1.00, 95% CI 0.95 to 1.05). To rule out a residual healthy donor effect (HDE), additional sensitivity analyses using a 5-year qualification period were conducted. The results supported the absence of a residual HDE.ConclusionsThis study showed a protective effect of long-term, high-frequency blood donation against cardiovascular disease. This effect was only observed in women and not in men.

scholarly journals Complete Microbiota Engraftment Is Not Essential for Recovery from Recurrent Clostridium difficile Infection following Fecal Microbiota Transplantation

mBio ◽  
2016 ◽  
Vol 7 (6) ◽  
Author(s):  
Christopher Staley ◽  
Colleen R. Kelly ◽  
Lawrence J. Brandt ◽  
Alexander Khoruts ◽  
Michael J. Sadowsky
Keyword(s):  
Clostridium Difficile ◽  
Bacterial Communities ◽  
Healthy Donor ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Mean Value ◽  
Bile Acid Metabolism ◽  
Secondary Bile Acid ◽  
Stool Samples ◽  
Relative Abundances

ABSTRACT Bacterial communities from subjects treated for recurrent Clostridium difficile infection (rCDI) by fecal microbiota transplantation (FMT), using either heterologous donor stool samples or autologous stool samples, were characterized by Illumina next-generation sequencing. As previously reported, the success of heterologous FMT (90%) was superior to that of autologous FMT (43%) ( P = 0.019), and post-FMT intestinal bacterial communities differed significantly between treatment arms ( P < 0.001). Subjects cured by autologous FMT typically had greater abundances of the Clostridium XIVa clade and Holdemania bacteria prior to treatment, and the relative abundances of these groups increased significantly after FMT compared to heterologous FMT and pre-FMT samples. The typical shift to post-FMT, donor-like assemblages, featuring high relative abundances of genera within the Bacteroidetes and Firmicutes phyla, was not observed in the autologous FMT subjects. Autologous FMT patient bacterial communities were significantly different in composition than those for heterologous FMT patients and donors ( P < 0.001). The SourceTracker program, which employs a Bayesian algorithm to determine source contributions to sink communities, showed that patients initially treated by heterologous FMT had significantly higher percentages of engraftment (i.e., similarity to donor communities, mean value of 74%) compared to those who suffered recurrence following autologous FMT (1%) ( P ≤ 0.013). The findings of this study suggest that complete donor engraftment may be not necessary if functionally critical taxa are present in subjects following antibiotic therapy. IMPORTANCE This study provides a detailed characterization of fecal bacterial communities in subjects who participated in a previously published randomized clinical trial to treat recurrent C. difficile infection (rCDI). Bacterial communities were characterized to determine differences between subjects who received fecal bacteria either from healthy donor stool samples or their own stool samples as “placebo” in order to determine which groups of bacteria were most important in achieving a cure. The results of this study suggested that bacteria associated with secondary bile acid metabolism could potentially provide resistance to infection and that complete transfer of healthy donor microorganisms was not necessary to resolve CDI following unsuccessful antibiotic treatment.

The healthy donor effect: a matter of selection bias and confounding

Transfusion ◽  
2011 ◽  
Vol 51 (9) ◽  
pp. 1883-1885 ◽  
Author(s):  
Femke Atsma ◽  
Femmie de Vegt
Keyword(s):  
Selection Bias ◽  
Healthy Donor
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