scholarly journals 496. Optimization of the FLPe/frt Recombination System for the Production of High-Capacity, Helper-Dependent Adenoviral (HD) Vector II: Detailed Molecular Analysis of the Stability of the Flpe/frt System over Repeated Passaging

2003 ◽  
Vol 7 (5) ◽  
pp. S194
Keyword(s):  
Molecular Analysis ◽  
High Capacity ◽  
Recombination System ◽  
The Stability ◽  
Detailed Molecular Analysis

scholarly journals Measurement of xylem water pressure using High-Capacity Tensiometer and benchmarking against Pressure Chamber and Thermocouple Psychrometer

2020 ◽  
Vol 195 ◽  
pp. 03014
Author(s):  
Roberta Dainese ◽  
Giuseppe Tedeschi ◽  
Thierry Fourcaud ◽  
Alessandro Tarantino
Keyword(s):  
Water Flow ◽  
Water Pressure ◽  
High Capacity ◽  
Ground Surface ◽  
Pressure Chamber ◽  
Rainwater Infiltration ◽  
Thermocouple Psychrometer ◽  
Earth Structures ◽  
Pressure Water ◽  
The Stability

The response of the shallow portion of the ground (vadose zone) and of earth structures is affected by the interaction with the atmosphere. Rainwater infiltration and evapotranspiration affect the stability of man-made and natural slopes and cause shallow foundations and embankments to settle and heave. Very frequently, the ground surface is covered by vegetation and, as a result, transpiration plays a major role in ground-atmosphere interaction. The soil, the plant, and the atmosphere form a continuous hydraulic system, which is referred to as Soil-Plant-Atmosphere Continuum (SPAC). The SPAC actually represents the ‘boundary condition’ of the geotechnical water flow problem. Water flow in soil and plant takes place because of gradients in hydraulic head triggered by the negative water pressure (water tension) generated in the leaf stomata. To study the response of the SPAC, (negative) water pressure needs to be measured not only in the soil but also in the plant. The paper presents a novel technique to measure the xylem water pressure based on the use of the High-Capacity Tensiometer (HCT), which is benchmarked against conventional techniques for xylem water pressure measurements, i.e. the Pressure Chamber (PC) and the Thermocouple Psychrometer (TP).

Prospects for the development of underground ore mining geotechnologies at the Talnakh and Oktyabrskoye deep mines

2021 ◽  
pp. 70-75
Author(s):  
I.I. Aynbinder ◽  
P.G. Patskevich ◽  
O.V. Ovcharenko
Keyword(s):  
High Capacity ◽  
Underground Mines ◽  
Total Production ◽  
Stress Concentrations ◽  
Mining System ◽  
Sulphide Ore ◽  
Rock Mass Deformation ◽  
The Rich ◽  
Context Mining ◽  
The Stability

Rich sulphide, cuprous and impregnated ores are currently mined in the underground mines of the Talnakh and Oktyabrskoye deposits at the depths from 250 to 1,700 m. The reserves of rich ores are depleted, and therefore the growth of cuprous and impregnated ores is gaining importance. Their share may reach 80% of the total production by 2030. A distinctive feature of such deposits is the occurrence of cuprous and impregnated ores above the rich sulphide ore, which reserves have been mined out using mining systems with curing backfill mixtures. In this context, mining of impregnated ores will be done in the undermined zones, which will lead to significant rock mass deformation, opening of existing natural and formation of new cracks, will affect the stability of mining structures and will require special measures to control rock pressure in the mines. The paper presents the results of assessing the stress-and-strain condition of the undermined mass of impregnated ores mined using the room-and-pillar cut-and-fill method at the depths of 500, 1000 and 2000 m. The assessment shows that no dangerous stress concentrations arise in the mining structures at great depths which creates preconditions for the safe development of such deposits. A significant increase in ore extraction will require upgrading of existing underground facilities. It is proposed to carry out pre-concentration of the mined ore in the underground conditions using modern crushing complexes, high-capacity mine separators to remove waste rock, which can subsequently be used as the backfill material. In this way, a closed-loop mining system is created that meets the efficiency requirements of mining production and integrated subsoil development.

Detailed molecular analysis of 1p36 in neuroblastoma

2001 ◽  
Vol 36 (1) ◽  
pp. 37-41 ◽  
Author(s):  
P.S. White ◽  
P.M. Thompson ◽  
B.A. Seifried ◽  
E.P. Sulman ◽  
S.J. Jensen ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Detailed Molecular Analysis

scholarly journals A detailed molecular analysis of complete Bovine Leukemia Virus genomes isolated from B-cell lymphosarcomas

2013 ◽  
Vol 44 (1) ◽  
pp. 19 ◽  
Author(s):  
Gonzalo Moratorio ◽  
Sabrina Fischer ◽  
Sergio Bianchi ◽  
Lorena Tomé ◽  
Gonzalo Rama ◽  
...  
Keyword(s):  
B Cell ◽  
Molecular Analysis ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Genomes ◽  
Detailed Molecular Analysis

scholarly journals Field and Numerical Modelling Investigations on the Stability of Underground Strata during Longwall Workings

2021 ◽  
Author(s):  
G Budi ◽  
Kolikipogu Nageswara Rao ◽  
Punit Mohanty
Keyword(s):  
Numerical Modelling ◽  
Longwall Mining ◽  
High Capacity ◽  
Field Monitoring ◽  
Longwall Face ◽  
Mining Method ◽  
Company Limited ◽  
Extensive Field ◽  
Powered Support ◽  
The Stability

Abstract Understanding the behaviour of underground workings is essential for the success of any mining method. The longwall mining method is one of the predominant underground methods to extract coal. Since 1978, in India, 22 underground coal mines of different collieries have been implemented the mechanized longwall method. SCCL is one of that colliery has mixed working experiences with longwall method in their mines. The longwall faces in GDK-10A, JK-5, and VK-7 of SCCL had produced good results, but the faces in GDK-7, GDK-9, GDK-11A, and PVK-5 had suffered due to the geological disturbances and unavailability of real-time information about the strata behaviour. By addressing the previous experiences of longwall workings, Singareni Collieries Company Limited (SCCL) has implemented a high capacity (1 × 1152T) powered support system in Adriyala Longwall Project (ALP) at a depth of 375m. In this study, extensive field monitoring with different strata monitoring instruments was conducted in ALP to analyze the gate roads convergence, stress variation on longwall and chain pillars at different stages of extraction (i.e., 8m, 25m, 35m, and 45m) and the pressure variation on the powered support systems. It was observed from the results that the convergence in the gate roads was increasing with the advance of the longwall face and the area of exposure. The pressure of the legs on the dip side was less than the pressure of the legs on the rise side, which implies a stable roof condition over the longwall face. To better understand the behaviour of ALP workings, a numerical modelling study with FLAC 7.0 has been conducted with actual physio-mechanical properties. The computed numerical modelling results have been remarkably well in consistent with the field monitoring results. The stability of chain pillars has been estimated at every stage of extraction by the Factor of Safety (FoS) criterion and it was found that the pillars could be ensured stability in longwall workings.

scholarly journals A New Generation of Dihydropyridine Calcium Channel Blockers: Photostabilization of Liquid Formulations Using Nonionic Surfactants

Pharmaceutics ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 28 ◽  
Author(s):  
Giuseppina Ioele ◽  
Miyase Gözde Gündüz ◽  
Claudia Spatari ◽  
Michele De Luca ◽  
Fedora Grande ◽  
...  
Keyword(s):  
Nonionic Surfactants ◽  
Water Solubility ◽  
High Capacity ◽  
Ethanol Solution ◽  
Multivariate Curve Resolution ◽  
Channel Blockers ◽  
Curve Resolution ◽  
Dihydropyridine Derivative ◽  
The Stability ◽  
New Generation

: The stability profile of a new 1,4-dihydropyridine derivative (DHP), representative of a series with a hexahydroquinoline ring, was studied to design light-stable liquid formulations. This molecule, named M3, has been shown among the analogs to have a high capacity to block both L- and T-type calcium channels. The ethanol solution of the drug was subjected to a photodegradation test, in accordance with standard rules. The concentrations of the drug and its byproducts were estimated using multivariate curve resolution, applied to the spectral data collected during the test. The improvement of both the photostability and water solubility of M3 was investigated by adding the surfactant polysorbate 20 in a 1:5 ratio to aqueous solutions of the drug. These formulations were exposed to stressing light in containers of bleu polyethylene terephthalate (PET), amber PET, and covered amber PET. The best results were obtained when using the covered amber PET container, reaching a degradation percentage of the drug less than 5% after 12 h under an irradiance power of 450 W/m2. The stability of the compound was compared to that of nimodipine (NIM) under the same conditions.

scholarly journals Detailed molecular analysis of the induction of the L-PK gene by glucose

2008 ◽  
Vol 372 (1) ◽  
pp. 131-136 ◽  
Author(s):  
David T. Eckert ◽  
Pili Zhang ◽  
J. Jason Collier ◽  
Robert M. O’Doherty ◽  
Donald K. Scott
Keyword(s):  
Molecular Analysis ◽  
Detailed Molecular Analysis

Incorporation of PEDOT:PSS into SnO2/reduced graphene oxide nanocomposite anodes for lithium-ion batteries to achieve ultra-high capacity and cyclic stability

RSC Advances ◽  
2015 ◽  
Vol 5 (18) ◽  
pp. 13964-13971 ◽  
Author(s):  
Md. Selim Arif Sher Shah ◽  
Shoaib Muhammad ◽  
Jong Hyeok Park ◽  
Won-Sub Yoon ◽  
Pil J. Yoo
Keyword(s):  
Graphene Oxide ◽  
Lithium Ion Batteries ◽  
Lithium Ion Battery ◽  
Reduced Graphene Oxide ◽  
Polymer Matrix ◽  
High Capacity ◽  
Lithium Ion ◽  
Cyclic Stability ◽  
Reduced Graphene ◽  
The Stability

A conducting polymer matrix of PEDOT:PSS is incorporated into SnO2/reduced graphene oxide composite for increasing the stability of lithium-ion battery anodes.

scholarly journals Molecular analysis of Saccharomyces cerevisiae chromosome I: identification of additional transcribed regions and demonstration that some encode essential functions.

Genetics ◽  
1991 ◽  
Vol 127 (2) ◽  
pp. 287-298 ◽  
Author(s):  
B E Diehl ◽  
J R Pringle
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Analysis ◽  
Unknown Function ◽  
Genetic Studies ◽  
Classical Genetic ◽  
Lethal Mutations ◽  
Integrative Transformation ◽  
Transformation Methods ◽  
Chromosome I ◽  
Detailed Molecular Analysis

Abstract Saccharomyces cerevisiae chromosome I has provided a vivid example of the "gene-number paradox." Although molecular studies have suggested that there are greater than 100 transcribed regions on the chromosome, classical genetic studies have identified only about 15 genes, including just 6 identified in intensive studies using Ts- lethal mutations. To help elucidate the reasons for this disparity, we have undertaken a detailed molecular analysis of a 34-kb segment of the left arm of the chromosome. This segment contains the four known genes CDC24, WHI1, CYC3 and PYK1 plus at least seven transcribed regions of unknown function. The 11 identified transcripts have a total length of approximately 25.9 kb, suggesting that greater than or equal to 75% of the DNA in this region is transcribed. Of the transcribed regions of unknown function, three are essential for viability on rich medium and three appear to be nonessential, as judged by the lethality or nonlethality of deletions constructed using integrative transformation methods. No obvious phenotypes were associated with the deletions in the apparently nonessential genes. However, two of these genes may have homologs elsewhere in the genome, as judged from the appearance of additional bands when DNA-DNA blot hybridizations were performed at reduced stringency. Taken together, the results provide further evidence that the limitations of classical genetic studies of chromosome I cannot be explained solely by a lack of genes, or even a lack of essential genes, on the chromosome.

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