Effect of atorvastatin on autoantibodies against oxidized low-density lipoprotein in postmenopausal women with coronary heart disease

Abstract Background The role of nuclear magnetic resonance (NMR) metabolomics in the prevention of coronary heart disease (CHD) in postmenopausal women is unclear.Objective To explore the NMR measured risk factors of CHD and the correlation among Gensini score, proprotein convertase subtilisin/kexin type-9 (PCSK9) and NMR metabolomics.Method 300 postmenopausal women who were under moderate intensity statins were enrolled and assigned into CHD Group (242) and non-CAHD Group (58). Multivariate Logistic regression and Spearman correlation analysis were conducted for the risk factors of CHD and the relationship among Gensini score, PCSK9 and NMR results in all patients as well as the patients with CHD, diabetes mellitus (DM) and metabolic syndrome (MS). ResultsAge, the particle of LDL-6, LDL-6- triglyceride (TG) and LDL-6-free cholesterol (FC) were risk factors of CHD, while, glycerol were the protective factors of CHD. Lipoprotein contents of very-low-density lipoprotein (VLDL)-2 ~ VLDL-5, intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL)-1 and LDL-2 were positively related to Gensini score, while the lipoprotein contents, apolipoprotein A1 (ApoA1) and ApoA2 of high-density lipoprotein (HDL)-1 ~ HDL-4 showed negative correlations with Gensini score. PCSK9 was negatively correlated to the particles of VLDL, IDL and LDL, total cholesterol (TC), ApoB/A1, and nearly all the lipid contents in VLDL-3 ~ VLDL-5, IDL and LDL-1.ConclusionIn postmenopausal women, under moderate intensity statins, age, and the NMR measured particle of LDL-6, LDL-6-TG and LDL-6-FC were risk factors of CHD, while glycerol were the protective factors of CHD. Lipoprotein contents of VLDL-2 ~ VLDL-5, IDL, LDL-1 and LDL-2 maybe the residual risk factors of CHD in postmenopausal women, who is under moderate intensive statin.

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Identification of oxidized low-density lipoprotein in human serum by NMR spectroscopy

Clinical Science ◽

10.1042/cs0950489 ◽

1998 ◽

Vol 95 (4) ◽

pp. 489-495 ◽

Cited By ~ 6

Author(s):

J. JANKOWSKI ◽

J.-R. NOFER ◽

M. TEPEL ◽

B. GRIEWEL ◽

H. SCHLÜTER ◽

...

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Human Serum ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Oxidized Low Density Lipoprotein ◽

Simple Method ◽

Control Subjects ◽

Healthy Control

1.In this study we compared the 500 MHz 1H-NMRs from native and oxidized low-density lipoproteins. 2.The measurements revealed a characteristic pattern of three resonances in spectra from oxidized, but not from native low-density liprotein at 1.17 p.p.m., 1.18 p.p.m. and 1.20 p.p.m. (relative to 3-trimethylsilyl-[2,2,3,3-2H4]-propionate). 3.A quantitative comparison between these resonances in sera from patients with coronary heart disease and healthy control subjects revealed that the intensity was significantly higher in patients with coronary heart disease (1.17 p.p.m.: 0.026±0.014 versus 0.015±0.019; 1.18 p.p.m.: 0.032±0.011 versus 0.017±0.021; 1.20 p.p.m.: 0.030±0.066 versus 0.010±0.005; P< 0.05 compared with healthy control subjects for each resonance). 4.Fractionation of sera from patients with coronary heart disease revealed that the resonances equal to those obtained from experimentally oxidized low-density lipoprotein are indeed caused by the low-density lipoprotein fraction of the sera. 5.When the NMRs from sera were calibrated with oxidized low-density lipoprotein prepared by Cu2+ oxidation, a concentration of 66.5±28.6 ;μg/ml and 36.3±23.7 ;μg/ml (P< 0.05) was estimated in patients with coronary heart disease and healthy subjects respectively. Elevated levels of oxidized low-density lipoprotein also occurred in those patients with normal serum concentrations of total low-density lipoprotein. 6.The study shows a simple method to measure oxidized low-density lipoprotein in human serum and may gain interest to assess the cardiovascular risk factor profiles more completely.

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