scholarly journals The effects of Trichoderma on preventing cucumber fusarium wilt and regulating cucumber physiology

2019 ◽  
Vol 18 (3) ◽  
pp. 607-617 ◽  
Author(s):  
Mei LI ◽  
Guang-shu MA ◽  
Hua LIAN ◽  
Xiao-lin SU ◽  
Ying TIAN ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Cucumber Fusarium Wilt

scholarly journals Rotations with Indian Mustard and Wild Rocket Suppressed Cucumber Fusarium Wilt Disease and Changed Rhizosphere Bacterial Communities

2019 ◽  
Vol 7 (2) ◽  
pp. 57 ◽  
Author(s):  
Xue Jin ◽  
Jian Wang ◽  
Dalong Li ◽  
Fengzhi Wu ◽  
Xingang Zhou
Keyword(s):  
Bacterial Community ◽  
Fusarium Wilt ◽  
Bacterial Community Composition ◽  
Indian Mustard ◽  
Wilt Disease ◽  
Crop Rotations ◽  
Pseudomonas Spp ◽  
Fusarium Wilt Disease ◽  
Wild Rocket ◽  
Cucumber Fusarium Wilt

Crop monocropping usually results in an enrichment of soil-borne pathogens in soil. Crop rotation is an environmentally friendly method for controlling soil-borne diseases. Plant rhizosphere microorganisms, especially plant-beneficial microorganisms, play a major role in protecting plants from pathogens, but responses of these microorganisms to crop rotation remain unclear. Here, we evaluated the effects of rotations with Indian mustard (Brassica juncea) and wild rocket (Diplotaxis tenuifolia (L.) DC.) on cucumber Fusarium wilt disease caused by Fusarium oxysporum f.sp. cucumerinum Owen (FOC). Cucumber rhizosphere bacterial community composition was analyzed by high-throughput amplicon sequencing. Bacteria, Pseudomonas spp., 2,4-diacetylphloroglucinol (an antifungal secondary metabolite) producer and FOC abundances were estimated by real-time PCR. Rotations with Indian mustard and wild rocket suppressed cucumber Fusarium wilt disease and cucumber rhizosphere FOC abundance. Crop rotations increased cucumber rhizosphere bacteria, Pseudomonas spp. and 2,4-diacetylphloroglucinol producer abundances. Moreover, crop rotations changed cucumber rhizosphere bacterial community composition and increased bacterial community diversity. However, crop rotations decreased soil inorganic nitrogen content and inhibited cucumber seedling growth. Overall, rotations with Indian mustard and wild rocket suppressed cucumber Fusarium wilt disease, which might be linked to the increased rhizosphere bacterial diversity and abundances of potential plant-beneficial microorganisms (such as Pseudomonas spp. and 2,4-diacetylphloroglucinol producer).

scholarly journals Allelopathic Control Effect of Complex Formulation of Compound Microbial Community and Bio-Organic Fertilizer on Cucumber fusarium Wilt

2020 ◽  
Vol 71 (4) ◽  
pp. 646-659
Author(s):  
Lijin Qin ◽  
Zengming Zhong ◽  
Dandan Wang ◽  
Haiqi Hu ◽  
Duo Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Cucumis Sativus ◽  
Fusarium Wilt ◽  
Organic Fertilizer ◽  
Disease Index ◽  
Allelopathic Effect ◽  
Soil Leaching ◽  
Control Effect ◽  
Complex Formulation ◽  
Cucumber Fusarium Wilt

To study the influence of complex formulation of �WoFengKang� compound microbial community and bio-organic fertilizer on allelopathic effect of cucumber fusarium wilt and field growth traits, in this experiment, different indoor treatments were taken to cultivate cucumber fusarium wilt using soil leaching liquor and Cucumis sativus growth index, fusarium wilt disease index were determined, so that allelopathic control effect of different dosages of compound microbial community on cucumber fusarium wilt can be investigated. The results showed that: compared with conventional CK, cucumber fusarium wilt cultured in different soil leaching liquor had smaller diameter. Where, colony diameter in treatment 3 was the smallest, which was 36.72 mm. Compound microbial community had certain allelopathic effect on cucumber fusarium wilt, and inhibition effect was shown with allelopathic effect reaching 27.13~43.91%. The above differently-treated soil was loaded to seedling-raising pot for Cucumis sativus planting. Cucumber fusarium wilt (FOC) was inoculated in the rough leaf stage of Cucumis sativus, thus reducing the disease index of cucumber fusarium wilt with obvious control effect reaching 55.94~72.63%. Where, treatment 3 demonstrated the best effect and allelopathic control effect reached 72.63% after 15d inoculation. The complex formulation of the two promoted vegetative growth of Cucumis sativus, lowered node of the first female flower bud, increased the number of female flowers within 30 nodes, so that 3.25d early flowering and 4.75d delayed seedling raising were achieved. Therefore, application of microbial community amid and after Cucumis sativus field planting has certain allelopathic control effect on cucumber fusarium wilt, which can effectively reduce the incidence of cucumber fusarium wilt and provide a scientific basis for the allelopathic prevention and control of soil-borne diseases in Cucumis sativus.

Arbuscular Mycorrhizal Fungal Inoculation Enhances Suppression of Cucumber Fusarium Wilt in Greenhouse Soils

Pedosphere ◽  
2010 ◽  
Vol 20 (5) ◽  
pp. 586-593 ◽  
Author(s):  
Jun-Li HU ◽  
Xian-Gui LIN ◽  
Jun-Hua WANG ◽  
Wei-Shou SHEN ◽  
Shu WU ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Arbuscular Mycorrhizal ◽  
Greenhouse Soils ◽  
Arbuscular Mycorrhizal Fungal ◽  
Fungal Inoculation ◽  
Cucumber Fusarium Wilt

scholarly journals Synergistic Effect of Dazomet Soil Fumigation and Clonostachys rosea Against Cucumber Fusarium Wilt

2014 ◽  
Vol 104 (12) ◽  
pp. 1314-1321 ◽  
Author(s):  
Tian Tian ◽  
Shi-Dong Li ◽  
Man-Hong Sun
Keyword(s):  
Synergistic Effect ◽  
Fusarium Wilt ◽  
Disease Incidence ◽  
Soil Fumigation ◽  
Control Measures ◽  
Clonostachys Rosea ◽  
Synergistic Mechanism ◽  
Colonization Potential ◽  
Sporulation Rate ◽  
Cucumber Fusarium Wilt

Soil fumigation and biological control are two control measures frequently used against soilborne diseases. In this study, the chemical fumigant dazomet was applied in combination with the biocontrol agent (BCA) Clonostachys rosea 67-1 to combat cucumber wilt caused by Fusarium oxysporum f. sp. cucumerinum KW2-1. When the mycoparasite C. rosea 67-1 was applied after dazomet fumigation, disease control reached 100%, compared with 88.1 and 69.8% for dazomet and 67-1 agent, respectively, applied alone, indicating a synergistic effect of dazomet and C. rosea in combating cucumber Fusarium wilt based on analysis of Bliss Independence. To understand the synergistic mechanism, the effects of chemical fumigation on the colonization potential and activity of F. oxysporum f. sp. cucumerinum, and the interaction between the BCA and the pathogen were investigated. The results showed that growth of the pathogen decreased with increasing dazomet concentration subsequent to fumigation. When exposed to dazomet at 100 ppm, the fungal sporulation rate decreased by 94.4%. Severe damage was observed in fumigated isolates using scanning electron microscopy. In the greenhouse, disease incidence of cucumber caused by fumigated F. oxysporum f. sp. cucumerinum significantly decreased. Whereas germination of C. rosea 67-1 spores increased by >sixfold in fumigated soil, and its ability to parasitize fumigated F. oxysporum f. sp. cucumerinum significantly increased (P = 0.014).

scholarly journals A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community

Microbiome ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Xianfeng Ye ◽  
Zhoukun Li ◽  
Xue Luo ◽  
Wenhui Wang ◽  
Yongkai Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Fusarium Wilt ◽  
Soil Microbial Community ◽  
Soil Microbial ◽  
Cucumber Fusarium Wilt

scholarly journals Screening of Cucumber Fusarium Wilt Bio-Inhibitor: High Sporulation Trichoderma harzianum Mutant Cultured on Moso Bamboo Medium

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Zhang ◽  
Hao Xu ◽  
Jingcong Xie ◽  
Jie-yu Cui ◽  
Jing Yang ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Irradiation Time ◽  
Moso Bamboo ◽  
Economic Losses ◽  
Original Strain ◽  
Beneficial Effects ◽  
Bamboo Stem ◽  
Cucumber Fusarium Wilt

Cucumber fusarium wilt is a soil-borne disease which causes serious production decrease in cucumber cultivation world widely. Extensive using of chemical pesticides has caused serious environmental pollution and economic losses, therefore, it is particularly urgent to develop efficient, safe and pollution-free biopesticide. In this study, a mutant strain of Trichoderma harzianum cultivated in moso bamboo medium was proved to be an efficient bio-inhibitor of the disease. The mutant strain T. harzianum T334, was obtained by three microwave mutagenesis cycles with an irradiation power of 600 W and irradiation time of 40 s. In contrast to the original strain, the inhibition rate on cucumber fusarium wilt of the strain T334 increased from 63 to 78%. In this work, disk milling pretreatment of moso bamboo has shown significant beneficial effects on both biotransformation and sporulation of T334. Its sporulation reached 3.7 × 109 cfu/g in mushroom bags with 90% bamboo stem powder (pretreated by disk milli), 9.5% bamboo leaf powder and 0.5% wheat bran when the ratio of solid to liquid was 4:6, the inoculum amount was 10%, and the culture temperature was 28°C. These results provide an alternative bioinhibitor for the control of cucumber fusarium wilt, and a potential usage of moso bamboo in the production of microbial pesticide.

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