Transient expression of activin βA mRNA on osteoprogenitor cells in rat bone regeneration after drill-hole injury

Bone ◽  
2000 ◽  
Vol 27 (1) ◽  
pp. 81-90 ◽  
Author(s):  
S Uchida ◽  
Y Doi ◽  
H Kudo ◽  
H Furukawa ◽  
T Nakamura ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Transient Expression ◽  
Drill Hole ◽  
Osteoprogenitor Cells ◽  
Rat Bone

Tissue-Engineered Hydroxyapatite Bone Scaffold Impregnated with Osteoprogenitor Cells Promotes Bone Regeneration in Sheep Model

2021 ◽  
Author(s):  
Mohd Yazid Bajuri ◽  
Nanchappan Selvanathan ◽  
Fatin Nadira Dzeidee Schaff ◽  
Muhammad Haziq Abdul Suki ◽  
Angela Min Hwei Ng
Keyword(s):  
Bone Regeneration ◽  
Bone Scaffold ◽  
Sheep Model ◽  
Osteoprogenitor Cells

scholarly journals Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
S. Lee ◽  
L. H. Remark ◽  
A. M. Josephson ◽  
K. Leclerc ◽  
E. Muiños Lopez ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Notch Signaling ◽  
Bone Regeneration ◽  
Bone Healing ◽  
Canonical Wnt Signaling ◽  
Osteoprogenitor Cells ◽  
Intramembranous Bone ◽  
Proliferation And Differentiation ◽  
Canonical Wnt ◽  
Wnt Signal

AbstractAdult bone regeneration is orchestrated by the precise actions of osteoprogenitor cells (OPCs). However, the mechanisms by which OPC proliferation and differentiation are linked and thereby regulated are yet to be defined. Here, we present evidence that during intramembranous bone formation OPC proliferation is controlled by Notch signaling, while differentiation is initiated by activation of canonical Wnt signaling. The temporospatial separation of Notch and Wnt signal activation during the early stages of bone regeneration suggests crosstalk between the two pathways. In vitro and in vivo manipulation of the two essential pathways demonstrate that Wnt activation leads to initiation of osteogenic differentiation and at the same time inhibits Notch signaling, which results in termination of the proliferative phase. Here, we establish canonical Wnt signaling as a key regulator that facilitates the crosstalk between OPC proliferation and differentiation during intramembranous, primary bone healing.

Cell-scaffold transplant of hydrogel seeded with rat bone marrow progenitors for bone regeneration

2011 ◽  
Vol 39 (5) ◽  
pp. 364-371 ◽  
Author(s):  
Dror Ben-David ◽  
Tali A. Kizhner ◽  
Thomas Kohler ◽  
Ralph Müller ◽  
Erella Livne ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Cell Scaffold ◽  
Rat Bone

scholarly journals Quantitative assessment of rat bone regeneration using complex master-slave optical coherence tomography

2019 ◽  
Vol 9 (5) ◽  
pp. 782-798 ◽  
Author(s):  
Ruxandra Elena Luca ◽  
Carmen Darinca Todea ◽  
Virgil-Florin Duma ◽  
Adrian Bradu ◽  
Adrian Gh. Podoleanu
Keyword(s):  
Optical Coherence Tomography ◽  
Bone Regeneration ◽  
Quantitative Assessment ◽  
Optical Coherence ◽  
Rat Bone

scholarly journals Formononetin, a methoxy isoflavone, enhances bone regeneration in a mouse model of cortical bone defect

2017 ◽  
Vol 117 (11) ◽  
pp. 1511-1522 ◽  
Author(s):  
Krishna Bhan Singh ◽  
Manisha Dixit ◽  
Kapil Dev ◽  
Rakesh Maurya ◽  
Divya Singh
Keyword(s):  
Bone Regeneration ◽  
Cortical Bone ◽  
Bone Defect ◽  
Bone Healing ◽  
Drill Hole ◽  
Repair Process ◽  
Fracture Repair ◽  
Injury Site ◽  
Healing Effect ◽  
Ct Analysis

AbstractThe bone regeneration and healing effect of formononetin was evaluated in a cortical bone defect model that predominantly heals by intramembranous ossification. For this study, female Balb/c mice were ovariectomised (OVx) and a drill-hole injury was generated in the midfemoral bones of all animals. Treatment with formononetin commenced the day after and continued for 21 d. Parathyroid hormone (PTH1–34) was used as a reference standard. Animals were killed at days 10 and 21. Femur bones were collected at the injury site for histomorphometry studies using microcomputed tomography (μCT) and confocal microscopy. RNA and protein were harvested from the region surrounding the drill-hole injury. For immunohistochemistry, 5 µm sections of decalcified femur bone adjoining the drill-hole site were cut.μCT analysis showed that formononetin promoted bone healing at days 10 and 21 and the healing effect observed was significantly better than in Ovx mice and equal to PTH treatment in many aspects. Formononetin also significantly enhanced bone regeneration as assessed by calcein-labelling studies. In addition, formononetin enhanced the expression of osteogenic markers at the injury site in a manner similar to PTH. Formononetin treatment also led to predominant runt-related transcription factor 2 and osteocalcin localisation at the injury site. These results support the potential of formononetin to be a bone-healing agent and are suggestive of its promising role in the fracture-repair process.

scholarly journals Guided bone regeneration using a hydrophilic membrane made of unsintered hydroxyapatite and poly(L-lactic acid) in a rat bone-defect model

2018 ◽  
Vol 37 (6) ◽  
pp. 912-918 ◽  
Author(s):  
Reo IKUMI ◽  
Takayuki MIYAHARA ◽  
Norio AKINO ◽  
Noriko TACHIKAWA ◽  
Shohei KASUGAI
Keyword(s):  
Lactic Acid ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Guided Bone Regeneration ◽  
Defect Model ◽  
Hydrophilic Membrane ◽  
Rat Bone

Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration

2014 ◽  
Vol 38 (9) ◽  
pp. 1779-1785 ◽  
Author(s):  
Yoshihiro Dogaki ◽  
Sang Yang Lee ◽  
Takahiro Niikura ◽  
Takashi Iwakura ◽  
Etsuko Okumachi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Osteoprogenitor Cells ◽  
Induced Pluripotent

scholarly journals Development and Bone Regeneration Capacity of Premixed Magnesium Phosphate Cement Pastes

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2119 ◽  
Author(s):  
Andrea Ewald ◽  
Dorothea Kreczy ◽  
Theresa Brückner ◽  
Uwe Gbureck ◽  
Melanie Bengel ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Femoral Condyle ◽  
Drill Hole ◽  
Magnesium Phosphate ◽  
Aqueous Environment ◽  
Regeneration Capacity ◽  
Cement Pastes ◽  
In Vivo Testing

Magnesium phosphate cements (MPC) have been demonstrated to have a superior bone regeneration capacity due to their good solubility under in vivo conditions. While in the past only aqueous MPC pastes have been applied, the current study describes the fabrication and in vitro/in vivo testing of an oil-based calcium doped magnesium phosphate (CaMgP) cement paste. Premixed oil-based pastes with CaMgP chemistry combine the advantages of conventional MPC such as high mechanical strength and good resorbability with a prolonged shelf-life and an easier clinical handling. The pastes set in an aqueous environment and predominantly form struvite and achieve a compressive strength of ~8–10 MPa after setting. The implantation into a drill-hole defect at the distal femoral condyle of New Zealand white rabbits over a course of 6 and 12 weeks demonstrated good biocompatibility of the materials without the formation of soft connective tissue or any signs of inflammation. In contrast to a hydroxyapatite forming reference paste, the premixed CaMgP pastes showed subsequent degradation and bony regeneration. The CaMgP cement pastes presented herein are promising bone replacement materials with excellent material properties for an improved and facilitated clinical application.

Effect of hypophysectomy on the proliferation and differentiation of rat bone marrow stromal cells

1999 ◽  
Vol 276 (1) ◽  
pp. E34-E42 ◽  
Author(s):  
James K. Yeh ◽  
Jodi F. Evans ◽  
Meng-Meng Chen ◽  
John F. Aloia
Keyword(s):  
Bone Marrow ◽  
Hormone Deficiency ◽  
Osteogenic Potential ◽  
Pituitary Hormone ◽  
Bone Marrow Stroma ◽  
Osteoprogenitor Cells ◽  
Proliferation And Differentiation ◽  
Marrow Stroma ◽  
Rat Bone

Conditions such as estrogen deficiency, skeletal unloading, and aging have all been demonstrated to have various effects on the proliferation and differentiation of bone marrow stroma-derived osteoprogenitor cells. Here we have sought to examine the effects of pituitary hormone deficiency on the proliferation and the differentiation of these osteoprogenitor cells using the hypophysectomized (HX) rat as a model. In the present study, we use an in vitro culture system to examine the effects of HX on the osteogenic potential of rat bone marrow stroma. With the intact animal as a control, we used [3H]thymidine incorporation and cell number as indexes of proliferation. We also measured alkaline phosphatase enzyme activity, relative levels of osteocalcin expression with RT-PCR, and osteopontin and bone sialoprotein steady-state levels by Northern blot to delineate the effect on differentiation. Our results indicate that osteoprogenitor cells exposed to a pituitary hormone-deficient environment in vivo demonstrate an enhanced proliferative capacity and also exhibit an augmented expression of differentiation markers when exposed to an optimal environment in vitro.

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